Gibberellin regulates the synthesis of stone cells in ‘Nanguo’ pear via the PuMYB91‐PuERF023 module
Physiologia Plantarum,
Год журнала:
2025,
Номер
177(1)
Опубликована: Янв. 1, 2025
Abstract
Stone
cells
are
one
of
the
limiting
factors
affecting
pear
fruit
quality
and
commodity
value.
The
formation
stone
cell
is
highly
correlated
with
lignin
deposition.
However,
molecular
mechanism
regulation
still
unclear.
Here,
we
observed
that
exogenous
application
GA
significantly
inhibited
also
decreased
content
in
‘Nanguo’
(
Pyrus
ussuriensis
)
fruits.
key
gene
PuPRX73
involved
synthesis
pathway
was
further
identified
using
RT‐PCR,
GA‐treatment
expression
.
Overexpression
or
silencing
fruits
increases
decreases
lignin.
We
transcription
PuMYB91
PuERF023
mRNA‐seq
their
after
GA‐treatment.
Transient
overexpression
promotes
fruits,
while
led
to
opposite
results
Yeast
one‐hybrid
(Y1H)
GUS
activity
analysis
revealed
directly
bind
activate
promoter,
co‐transfection
Nicotiana
benthamiana
leaves
promoted
promoter
Furthermore,
found
interacted
vitro
by
two‐hybrid
assays
(Y2H).
In
conclusion,
our
inhibits
production
suppressing
Язык: Английский
The transcription factor CsPAT1 from tea plant (Camellia sinensis) is involved in drought tolerance by modulating phenylpropanoid biosynthesis
Journal of Plant Physiology,
Год журнала:
2025,
Номер
unknown, С. 154474 - 154474
Опубликована: Март 1, 2025
Язык: Английский
Not just flavor: Insights into the metabolism of tea plants
Current Opinion in Plant Biology,
Год журнала:
2025,
Номер
85, С. 102716 - 102716
Опубликована: Апрель 7, 2025
Язык: Английский
Circadian rhythm response and its effect on photosynthetic characteristics of the Lhcb family genes in tea plant
BMC Plant Biology,
Год журнала:
2024,
Номер
24(1)
Опубликована: Апрель 25, 2024
The
circadian
clock,
also
known
as
the
rhythm,
is
responsible
for
predicting
daily
and
seasonal
changes
in
environment,
adjusting
various
physiological
developmental
processes
to
appropriate
times
during
plant
growth
development.
clock
controls
expression
of
Lhcb
gene,
which
encodes
chlorophyll
a/b
binding
protein.
However,
roles
gene
tea
remain
unclear.
Язык: Английский
The GRAS transcription factor PtrPAT1 of Poncirus trifoliata functions in cold tolerance and modulates glycine betaine content by regulating the BADH-like gene
Horticulture Research,
Год журнала:
2024,
Номер
12(1)
Опубликована: Окт. 23, 2024
Abstract
GRAS,
termed
after
gibberellic
acid
insensitive
(GAI),
RGA
(repressor
of
GA1),
and
SCR
(scarecrow),
is
a
plant-specific
transcription
factor
crucial
for
plant
development
stress
response.
However,
understanding
the
functions
played
by
GRAS
members
their
target
genes
in
citrus
limited.
In
this
study,
we
identified
cold
stress-responsive
gene
from
Poncirus
trifoliata,
designated
as
PtrPAT1,
yeast
one-hybrid
library
screening
using
promoter
PtrBADH-l,
betaine
aldehyde
dehydrogenase
(BADH)-like
gene.
belonging
to
PAT1
subfamily,
was
localized
nucleus
plasma
membrane,
exhibited
transactivation
activity
showed
remarkable
upregulation
under
stress.
Overexpression
PtrPAT1
elevated
BADH
activity,
increased
glycine
(GB)
accumulation,
conferred
enhanced
tolerance
transgenic
tobacco
plants
compared
with
wild
type,
while
downregulation
trifoliate
orange
virus-induced
silencing
(VIGS)
resulted
opposite
trends.
Furthermore,
activities
two
antioxidant
enzymes,
including
peroxidase
(POD)
superoxide
dismutase
(SOD),
were
significantly
overexpression
plants,
but
remarkably
decreased
VIGS
line,
consistent
accumulation
patterns
reactive
oxygen
species
(ROSs).
demonstrated
interact
activate
PtrBADH-l
through
putative
PAT1-binding
motif
core
sequence
TTTCATGT,
indicating
that
PtrPAT1.
Taken
together,
these
results
demonstrate
positively
affects
regulation
GB
biosynthesis
modulating
expression.
Язык: Английский