Chromosome‐level assembly of basil genome unveils the genetic variation driving Genovese and Thai aroma types
Jing Zhang,
Mohamad Abu‐Abied,
Renana Milavski
и другие.
The Plant Journal,
Год журнала:
2025,
Номер
121(2)
Опубликована: Янв. 1, 2025
Basil,
Ocimum
basilicum
L.,
is
a
widely
cultivated
aromatic
herb,
prized
for
its
culinary
and
medicinal
uses,
predominantly
owing
to
unique
aroma,
primarily
determined
by
eugenol
Genovese
cultivars
or
methyl
chavicol
Thai
cultivars.
To
date,
comprehensive
basil
reference
genome
has
been
lacking,
with
only
fragmented
draft
available.
fill
this
gap,
we
employed
PacBio
HiFi
Hi-C
sequencing
construct
homeolog-phased
chromosome-level
basil.
The
tetraploid
was
assembled
into
26
pseudomolecules
further
categorized
subgenomes.
High
levels
of
synteny
were
observed
between
the
two
subgenomes
but
comparisons
Salvia
rosmarinus
show
collinearity
quickly
breaks
down
in
near
relatives.
We
utilized
bi-parental
population
derived
from
×
cross
map
quantitative
trait
loci
(QTL)
aroma
chemotype.
discovered
single
QTL
governing
eugenol/methyl
ratio,
which
encompassed
genomic
region
95
genes,
including
15
genes
encoding
shikimate
O-hydroxycinnamoyltransferase
(HCT/CST)
enzyme.
Of
them,
ObHCT1
exhibited
significantly
higher
expression
cultivar
showed
trichome-specific
expression.
functionally
confirmed
as
genuine
HCT
enzyme
using
an
vitro
assay.
high-quality,
contiguous
now
publicly
accessible
at
BasilBase,
valuable
resource
scientific
community.
Combined
insights
cell-type-specific
gene
expression,
it
promises
elucidate
specialized
metabolite
biosynthesis
pathways
cellular
level.
Язык: Английский
Current insights into plant volatile organic compound biosynthesis
Current Opinion in Plant Biology,
Год журнала:
2025,
Номер
85, С. 102708 - 102708
Опубликована: Март 28, 2025
Язык: Английский
Transient expression of PRISEs and Trichoderma-mediated elicitation promote iridoid production in Nepeta sibirica L.
Plant Physiology and Biochemistry,
Год журнала:
2025,
Номер
225, С. 109986 - 109986
Опубликована: Май 5, 2025
Язык: Английский
Pseudomolecule-scale genome assemblies of Drepanocaryum sewerzowii and Marmoritis complanata
G3 Genes Genomes Genetics,
Год журнала:
2024,
Номер
14(10)
Опубликована: Июль 24, 2024
Abstract
The
Nepetoideae,
a
subfamily
of
Lamiaceae
(mint
family),
is
rich
in
aromatic
plants,
many
which
are
sought
after
for
their
use
as
flavors
and
fragrances
or
medicinal
properties.
Here,
we
present
genome
assemblies
two
species
Nepetiodeae:
Drepanocaruym
sewerzowii
Marmoritis
complanata.
Both
were
generated
using
Oxford
Nanopore
Q20
+
reads
with
contigs
anchored
to
nine
pseudomolecules
that
resulted
335
Mb
305
assemblies,
respectively,
BUSCO
scores
above
95%
both
the
assembly
annotation.
We
furthermore
provide
tree
only
genome-derived
gene
models,
complementing
existing
transcriptome
marker-based
phylogenies.
Язык: Английский
Genome Report: Pseudomolecule-scale genome assemblies ofDrepanocaryum sewerzowiiandMarmoritis complanata
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 28, 2024
Abstract
The
Nepetoideae,
a
subfamily
of
Lamiaceae
(mint
family),
is
rich
in
aromatic
plants,
many
which
are
sought
after
for
their
use
as
flavours
and
fragrances
or
medicinal
properties.
Here
we
present
genome
assemblies
two
species
Nepetiodeae:
Drepanocaruym
sewerzowii
Marmoritis
complanata
.
Both
were
generated
using
Oxford
Nanopore
Q20+
reads
with
contigs
anchored
to
nine
pseudomolecules
that
resulted
335
Mb
305
assemblies,
respectively,
BUSCO
scores
above
95%
both
the
assembly
annotation.
We
furthermore
provide
tree
only
derived
gene
models,
complementing
existing
transcriptome
marker-based
phylogenies.
Язык: Английский