Amino acid substrate specificities and tissue expression profiles of the nine CYP79A encoding genes in Sorghum bicolor
Physiologia Plantarum,
Год журнала:
2025,
Номер
177(1)
Опубликована: Янв. 1, 2025
Abstract
Cytochrome
P450s
of
the
CYP79
family
catalyze
two
N‐hydroxylation
reactions,
converting
a
selected
number
amino
acids
into
corresponding
oximes.
The
sorghum
genome
(
Sorghum
bicolor
)
harbours
nine
CYP79A
encoding
genes,
and
here
sequence
comparisons
CYP79As
along
with
their
substrate
recognition
sites
(SRSs)
are
provided.
specificity
previously
uncharacterized
was
investigated
by
transient
expression
in
Nicotiana
benthamiana
subsequent
transformation
oximes
formed
stable
oxime
glucosides
catalyzed
endogenous
UDPG‐glucosyltransferases
(UGTs).
CYP79A61
uses
phenylalanine
as
substrate,
whereas
CYP79A91,
CYP79A93,
CYP79A95
use
valine
isoleucine
substrates,
CYP79A93
showing
ability
also
to
phenylalanine.
CYP79A94
substrate.
Analysis
249
transcriptomes
from
different
cultivars
showed
levels
tissue‐specific
s.
CYP79A1
is
committed
gene
dhurrin
formation
highest
expressed
most
tissues/organs.
primarily
fully
developed
leaf
blades
sheaths.
CYP79A91
CYP79A92
were
mainly
roots
>200
cm
below
ground,
while
highly
collar
sheath,
respectively.
possible
signalling
effects
metabolites
produced
tissues
discussed.
Язык: Английский
The case for sporadic cyanogenic glycoside evolution in plants
Current Opinion in Plant Biology,
Год журнала:
2024,
Номер
81, С. 102608 - 102608
Опубликована: Июль 31, 2024
Cyanogenic
glycosides
are
α-hydroxynitrile
glucosides
present
in
approximately
3000
different
plant
species.
Upon
tissue
disruption,
cyanogenic
hydrolyzed
to
release
toxic
hydrogen
cyanide
as
a
means
of
chemical
defense.
Over
100
have
been
reported,
with
structural
diversity
dependent
on
the
precursor
amino
acid,
and
subsequent
modifications.
represent
prime
example
sporadic
metabolite
evolution,
metabolic
trait
arising
multiple
times
throughout
lineage
evidenced
by
recruitment
enzyme
families
for
biosynthesis.
Here,
we
review
latest
developments
within
glycoside
biosynthesis,
argue
possible
factors
driving
evolution
including
shared
intermediates
crossovers
other
pathways
crossovers,
multifunctionality
beyond
Язык: Английский
Harnessing the Power of an Extensive EMS‐Induced Sorghum Population for Rapid Crop Improvement
Physiologia Plantarum,
Год журнала:
2024,
Номер
176(4)
Опубликована: Июль 1, 2024
Plant
breeders
leverage
mutagenesis
using
chemical,
biological,
and
physical
mutagens
to
create
novel
trait
variations.
Many
widely
used
sorghum
genotypes
have
a
narrow
genetic
base,
which
hinders
improvements
classical
breeding.
Enhancing
the
diversity
of
genome
thus
remains
key
priority
for
breeders.
To
accelerate
enhancement
sorghum,
an
extensive
library
comprised
seeds
from
150,000
individual
mutant
plants
Sorghum
bicolor
inbred
line
BTx623
was
established
ethyl
methanesulphonate
(EMS)
as
mutagen.
The
bulked
into
1498
pools
(~100
seed
heads
per
pool).
In
each
pool,
DNA
extracted
subset
screened
FIND-IT
technology
based
on
droplet
digital
PCR.
All
43
nucleotide
substitutions
that
were
identified,
demonstrating
potential
identify
any
EMS-derived
mutation
in
elite
within
days.
This
diverse
represents
largest
collection
mutants
ever
conceived,
estimated
cover
240%
all
possible
EMS-induced
points
genome.
Using
FIND-IT,
speed
at
specific
desired
can
be
identified
is
major
upgrade
conventional
reverse
techniques.
Additionally,
ease
valuable
variants
integrated
commercial
lines
far
simpler
less
expensive
process
compared
editing.
Genomic
variations
will
direct
utility
breeding
resource
applications,
allowing
enhanced
adaptation
climate
change
yield
marginal
environments.
Язык: Английский
Sequence diversity in the monooxygenases involved in oxime production in plant defense and signaling: a conservative revision in the nomenclature of the highly complex CYP79 family
The Plant Journal,
Год журнала:
2024,
Номер
120(3), С. 1236 - 1256
Опубликована: Окт. 22, 2024
SUMMARY
Cytochrome
P450
monooxygenases
of
the
CYP79
family
catalyze
conversion
specific
amino
acids
into
oximes
feeding
a
variety
metabolic
plant
pathways.
Here
we
present
an
extensive
phylogenetic
tree
built
on
carefully
curated
sequences
collected
across
entire
kingdom.
Based
monophyletic
origin
P450s,
set
evolutionarily
distinct
branches
was
identified.
Founded
functionally
characterized
sequences,
sequence
features
individual
substrate
recognition
sites
(SRSs)
were
analyzed.
Co‐evolving
acid
residues
identified
using
co‐evolutionary
analysis.
SRS4
possesses
pattern
when
tyrosine
is
substrate.
Except
for
CYP79Cs
and
CYP79Fs,
preferences
toward
could
not
be
assigned
to
subfamilies.
The
highly
diversified
tree,
reflecting
recurrent
independent
evolution
CYP79s,
may
relate
different
roles
in
species.
differences
subfamilies
facilitate
vivo
orchestration
channeled
pathways
based
altered
surface
charge
domains
protein.
Alternatively,
they
serve
optimize
dynamic
interactions
with
oxime
metabolizing
enzymes
enable
optimal
ecological
interactions.
outlined
detailed
curation
used
building
made
it
appropriate
make
conservative
tree‐based
revision
naming
within
this
complex
cytochrome
family.
same
approach
other
phylogeny
will
further
exploration
function
these
enzymes.
Язык: Английский