Combined analysis of metabolomic, transcriptomic and proteomics to reveal the underlying mechanism of mucilage disappearance in Brasenia schreberi

Industrial Crops and Products, Год журнала: 2025, Номер 232, С. 121178 - 121178

Опубликована: Май 22, 2025

Язык: Английский

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MsAREB1 enhances combined cold and saline–alkali stress tolerance by promoting ascorbic acid biosynthesis in alfalfa

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Май 26, 2025

Summary Cold and saline–alkali stress are typical abiotic stresses on forage in middle high latitudes, they frequently occur simultaneously, decreasing the yield quality of forage. Ascorbic acid plays an essential role reactive oxygen species metabolism response to stress. However, molecular mechanisms ascorbic biosynthesis induced by combined cold remain unclear. This study identified abscisic acid‐responsive element‐binding protein/ABRE‐binding factors transcription factor (TF), MsAREB1, which was significantly treatment. Under stress, MsAREB1 overexpression regulated played a defence positively regulating myo‐inositol oxygenase 2 ( MsMIOX2 ) expression. improved resistance increasing content. In addition, MsILR3, bHLH TF, interacted with form protein complex, thereby reducing MsAREB1‐induced transcriptional activation . demonstrates model for regulatory mechanism MsAREB1‐mediated regulation expression reduce oxidative damage These results provide insights improving plants lay foundation genetic improvement tolerance alfalfa.

Язык: Английский

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Genome-wide identification of the trehalose-6-phosphate synthase gene family in alfalfa (Medicago sativa L.) reveals involvement of MsTPS16 in tolerance to saline–alkali stress

Gene, Год журнала: 2025, Номер unknown, С. 149609 - 149609

Опубликована: Июнь 1, 2025

Язык: Английский

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Integrated transcriptomic and metabolomic analyses provide new insights into alkaline stress tolerance in Gossypium hirsutum

Frontiers in Plant Science, Год журнала: 2025, Номер 16

Опубликована: Июнь 3, 2025

Introduction Cotton, one of the most important economic crops worldwide, has long been bred mainly for improvements in yield and quality, with relatively little focus on salt–alkali resistance. Methods In this study, transcriptomic metabolomic sequencing were performed Gossypium hirsutum exposed to alkaline stress different durations. Results The results sample clustering, principal component analysis (PCA), number differentially expressed genes (DEGs) revealed that 12 hours 24 periods during which upland cotton presented strongest response salt stress, flavonoid biosynthesis alpha-linolenic acid metabolism playing significant roles time. A total 6,610 DEGs identified via comparison 0 h time point, including 579 transcription factors (TFs) significantly enriched pathways such as biosynthesis, cell cycle, cytochrome P450 pathway, phenylalanine metabolism, phototransduction, metabolism. Through ultrahigh-performance liquid chromatography–MS (UPLC-MS), 4,225 metabolites identified, 1,684 accumulated (DAMs) by levels at h. joint RNA-seq data play key G. these identified. weighted gene correlation network (WGCNA) 15 candidate associated alkali tolerance cotton, 4 TFs related anthocyanin biosynthesis. Conclusion conclusion, our study provides a theoretical foundation understanding molecular mechanisms underlying offers new resources future research.

Язык: Английский

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Intragenic cytosine methylation modification regulates the response of SUCLα1 to lower temperature in Solanaceae

Plant Science, Год журнала: 2024, Номер 350, С. 112320 - 112320

Опубликована: Ноя. 13, 2024

Язык: Английский

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