Identification and analysis of miRNA - mRNA regulatory modules associated with resistance to bacterial leaf streak in rice
BMC Genomics,
Год журнала:
2025,
Номер
26(1)
Опубликована: Март 2, 2025
acterial
leaf
streak
(BLS)
is
a
bacterial
disease
that
severely
affects
rice
leaves,
leading
to
significant
yield
reductions.
microRNAs
(miRNAs)
are
short
non-coding
RNAs
extensively
involved
in
the
growth,
development,
and
stress
responses
of
plants
animals.
However,
miRNAs
regulate
response
still
relatively
scarce.
The
indica
variety
Dular
exhibits
resistance
BLS,
whereas
9311
highly
susceptible
disease.
By
conducting
miRNA
sequencing
transcriptome
on
both
before
after
BLS
inoculation,
we
identified
19
were
significantly
downregulated
at
12
24
h
post-inoculation
Dular,
9
upregulated
same
time
points
9311.
Additionally,
through
degradome
sequencing,
23
miRNA-
mRNA
regulatory
modules
likely
play
crucial
roles
4
may
be
important
susceptibility
Current
studies
primarily
focus
those
blast
blight,
with
miRNA-target
mechanisms
for
remaining
unclear.
This
study
has
miRNA-mRNA
contributing
understanding
network
defense
against
infection.
Язык: Английский
DSF‐inspired discovery of novel zingerone‐based quorum‐sensing inhibitors: an attractive tactic of fighting Xanthomonas bacterial infections
Pest Management Science,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 26, 2025
Plant
bacterial
diseases
have
precipitated
a
global
crisis
in
human
health
and
food
security.
In
agriculture,
such
as
the
rice
white-leaf
blight
can
cause
considerable
reduction
crop
yields
and,
extreme
cases,
complete
failure.
Therefore,
there
is
an
urgent
need
for
novel
green
pesticides
with
innovative
modes-of-action
(MoAs).
A
series
of
zingerone
derivatives
isopropanolamine
skeleton
were
designed
their
quorum-sensing
effects
estimated
using
biomimetic
strategies.
The
results
showed
that
compound
A12
effectively
inhibits
growth
Xanthomonas
oryzae
pv.
[Xoo,
median
effective
concentration
(EC50)
=
3.55
μg
mL-1],
axonopodis
citri
(Xac,
EC50
3.23
mL-1)
oryzicola
(Xoc,
7.48
mL-1).
Molecular
docking
revealed
forms
multiple
interactions
RpfF
protein,
while
fluorescence
titration
experiments
further
verified
interaction
between
them.
Additionally,
was
found
to
inhibit
Xoo
biofilm
formation,
extracellular
polysaccharide
production
other
virulence
factors.
vivo
pot
exhibited
excellent
curative
(53.59%)
protective
(57.65%)
activity
against
Xoo.
conclusion,
A12's
primary
MoA
achieved
by
affecting
formation
biofilms
expression
various
factors,
including
related
genes.
summary,
this
study
highlights
displays
notable
sensing
activity.
advancement
inhibitors
through
strategies
offers
promising
avenues
future
research.
©
2025
Society
Chemical
Industry.
Язык: Английский
Recent advances in innovative strategies for plant disease resistance breeding
Frontiers in Plant Science,
Год журнала:
2025,
Номер
16
Опубликована: Май 21, 2025
Plant
disease
poses
a
great
threat
to
crop
production.
The
mechanisms
underlying
plant-pathogen
interactions
are
critical
research
topics
worldwide.
In
recent
years,
significant
breakthrough
studies
have
been
reported,
broadening
our
understanding
of
plant
immunity.
Based
on
these
findings,
many
strategies
developed
improve
defense
against
various
diseases.
Here,
we
summarize
and
their
applications
in
aimed
at
promoting
resistance.
Besides
domain
swapping,
gene
shuffling,
random
mutation,
three
additional
the
last
decade.
first
strategy
is
editing
host
susceptibility
(S)
genes
prevent
pathogen
infection.
Editing
Mlo
DMR6
species
good
examples
this
approach.
second
promoters
S
or
resistance
(R)
genes.
This
widely
used
counteract
Xanthomonas,
such
as
modifying
LOB1
SWEET
several
crops
enhance
third
designing
R
products,
especially
nucleotide-binding
leucine-rich
repeat
(NLR)
receptors.
approach
based
growing
knowledge
structural
features
NLRs,
which
seen
advances
recently.
To
date,
all
NLR-engineering
attempts
focused
rice
paired
Pikp-1/Pikp-2
(allelic
Pikm-1/Pikm-2)
RGA4/RGA5.
bioengineering
NLRs
provides
promising
method
combat
diverse
pathogens.
Detailed
also
discussed
review,
organized
around
strategies.
summary,
with
progresses
immune
mechanism,
innovative
molecular
available
mitigate
pathogens
future.
Язык: Английский
Type III Secretion Effectors of Xanthomonas oryzae pv. oryzicola: The Arsenal to Attack Equivalent Rice Defense for Invasion
Agronomy,
Год журнала:
2024,
Номер
14(9), С. 1881 - 1881
Опубликована: Авг. 23, 2024
Rice–Xanthomonas
oryzae
pv.
oryzicola
(Xoc)
is
one
of
the
commonly
used
rice
models
host–pathogen
interactions.
Xoc
causes
bacterial
leaf
streak
(BLS)
and
has
quarantine
status.
As
a
Gram-negative
pathogen,
usually
employs
type
III
secretion
effectors
(T3SEs),
including
transcription
activator-like
(TALEs)
non-TALEs,
to
interfere
with
innate
immunity
rice.
However,
few
major
resistance
genes
corresponding
are
found
in
cultivations;
only
Rxo1-AvrRxo1
Xo1-TALEs
interactions
have
been
discovered
rice–Xoc.
In
this
review,
we
focus
on
role
T3S
system
(T3SS)
virulence
consider
reported
AvrRxo1,
AvrBs2,
XopN,
XopC2,
XopAP,
XopAK,
as
well
TALEs
Tal2g/Tal5d,
Tal2h,
Tal2a,
Tal7,
Tal10a,
TalI,
Tal2b,
Tal2c.
Interestingly,
XopAP
disturb
stomatal
opening
promote
infection
through
targeting
diverse
signaling
pathways
Otherwise,
Tal2b
Tal2c,
respectively,
activate
two
salicylic
acid
(SA)
hydroxylation
redundantly
suppress
SA-mediated
basal
defense,
which
unknown
targets,
suppresses
SA
pathway
addition,
other
factors
discussed.
conclusion,
several
T3SEs
from
similar
defense
achieve
invasion,
providing
an
outlook
for
control
disease
manipulating
conserved
pathways.
Язык: Английский
Ancient duplications, multidimensional specializations, and defense role of hexokinases in wheat
Xiaolin Tian,
Fan Li,
Jie Lin
и другие.
The Plant Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 4, 2024
SUMMARY
Hexokinases
(HXKs),
which
sense
and
catalyze
cellular
sugar,
play
a
critical
role
in
the
growth
development
of
various
plants,
including
wheat,
primary
source
human
calories
frequently
attacked
by
fungal
pathogens.
However,
evolutionary
dynamics
functional
diversification
HXKs
particularly
their
roles
plant
defense,
remain
unclear.
Here,
we
discovered
that
wheat
hexokinase
gene
family
originated
through
multiple
ancient
duplications
across
different
lineages
has
undergone
comprehensive,
multidimensional
specialization
expression,
subcellular
localization,
enzyme
activity,
regulation
defense
responses.
Gene
expression
analysis
suggests
two‐thirds
TaHXK
genes
are
responsive
to
infection.
Subcellular
reveals
while
six
TaHXKs
localized
mitochondria,
three
from
phylogenetic
branches
sorted
into
other
compartments.
Notably,
biochemical
shows
mitochondria
differ
glucose‐catalyzing
with
TaHXK5
TaHXK3
exhibiting
highest
lowest
respectively.
Consistently,
transient
induces
responses,
is
defective
activating
some
Furthermore,
inactivation
glucokinase
activity
compromised
its
function
activation,
suggesting
mitochondrial
display
divergence
both
defense‐inducing
intrinsically
connected.
Overall,
our
findings
reveal
events
following
duplication
may
have
shaped
diversity
shedding
light
on
potentially
contributing
improvement
defense.
Язык: Английский