A Comprehensive Review of Plant Volatile Terpenoids, Elucidating Interactions with Surroundings, Systematic Synthesis, Regulation, and Targeted Engineering Production
Biology,
Год журнала:
2025,
Номер
14(5), С. 466 - 466
Опубликована: Апрель 25, 2025
Plants
require
a
flexible
avoidance
mechanism
as
they
need
to
cope
with
external
stimuli
and
challenges
through
complex
specialized
metabolites,
among
which
volatile
terpenoids
make
outstanding
contributions,
acting
key
media
signal
substances
in
the
cooperation
between
plants
surrounding
organisms.
In
recent
decades,
research
on
identification
functional
characterization
of
terpenoid
synthase
factors
regulating
metabolic
shunts
has
gained
significant
attention,
leading
substantial
progress
notable
achievements.
However,
popularization
insect
disease
prevention,
medical
care,
cosmetics,
other
fields,
coupled
increasing
resistance
artificially
produced
chemical
products,
demand
for
natural
outpaced
supply,
prompting
emergence
popularity
targeted
engineering
mass
production
using
microorganisms
platforms.
this
paper,
we
provide
detailed
overview
knowledge
regard
multiple
functions,
synthetic
pathways,
genes,
related
regulatory
factors,
target
engineering.
Язык: Английский
Functional Characterization of 5-O-Glycosyltranferase Transforming 3-O Anthocyanins into 3,5-O Anthocyanins in Freesia hybrida
International Journal of Molecular Sciences,
Год журнала:
2025,
Номер
26(10), С. 4542 - 4542
Опубликована: Май 9, 2025
Floral
coloration
in
Freesia
hybrida
is
predominantly
attributed
to
anthocyanins,
with
glycosylation
playing
a
critical
role
their
stability
and
diversity.
This
study
investigates
the
molecular
mechanisms
underlying
color
variation
between
F.
cultivars,
focusing
on
anthocyanin
5-O-glucosyltransferases
(An5GTs).
HPLC
analysis
revealed
that
‘Pink
Passion’
petals
accumulate
3,5-O-diglucosidic
absent
‘Red
River®’
‘Ambiance’.
RNA-seq
identified
seven
candidate
Fh5GT
genes,
phylogenetic
subcellular
localization
analyses
confirming
classification
as
cytosolic
glycosyltransferases.
Expression
profiling
highlighted
elevated
transcript
levels
of
Fh5GT1,
Fh5GT3,
Fh5GT7
Passion’,
correlating
its
di-glucosidic
accumulation.
In
vitro
enzymatic
assays
demonstrated
Fh5GT3
preferentially
glucosylate
3-O-monoglucosidic
anthocyanins
form
stable
3,5-O-diglucosides,
minimal
activity
anthocyanidins
generate
5-O-glucosidic
anthocyanins.
Heterologous
expression
Arabidopsis
complemented
deficiency
5gt
mutants,
restoring
pigmentation.
These
findings
elucidate
potential
5GTs
modulating
floral
diversity
through
modification,
providing
insights
for
targeted
breeding
strategies
enhance
ornamental
traits
horticultural
species.
Язык: Английский