A Novel Functional Method of Protector Screening for Zebrafish Lateral Line Hair Cells via the Acoustic Escape Response
Neuroscience Bulletin,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 6, 2025
Язык: Английский
Hair cell toxicology: With the help of a little fish
Frontiers in Cell and Developmental Biology,
Год журнала:
2022,
Номер
10
Опубликована: Дек. 13, 2022
Hearing
or
balance
loss
are
disabling
conditions
that
have
a
serious
impact
in
those
suffering
them,
especially
when
they
appear
children.
Their
ultimate
cause
is
frequently
the
of
function
mechanosensory
hair
cells
inner
ear.
Hair
can
be
damaged
by
environmental
insults,
like
noise
chemical
agents,
known
as
ototoxins.
Two
most
common
ototoxins
life-saving
medications:
cisplatin
against
solid
tumors,
and
aminoglycoside
antibiotics
to
treat
infections.
However,
due
their
localization
inside
temporal
bone,
difficult
study
mammals.
As
an
alternative
animal
model,
zebrafish
larvae
similar
mammals,
some
which
located
fish
specific
organ
on
surface
skin,
lateral
line.
This
makes
them
easy
observe
Язык: Английский
Physiological responses of mechanosensory systems in the head of larval zebrafish (Danio rerio)
Frontiers in Robotics and AI,
Год журнала:
2023,
Номер
10
Опубликована: Июль 31, 2023
The
lateral
line
system
of
zebrafish
consists
the
anterior
line,
with
neuromasts
distributed
on
head,
and
posterior
trunk.
sensory
afferent
neurons
are
contained
in
ganglia,
respectively.
So
far,
vast
majority
physiological
developmental
studies
have
focused
line.
However,
that
focus
especially
its
physiology,
very
rare.
involves
different
neuromast
patterning
processes,
specific
distribution
synapses,
a
unique
role
behavior.
Here,
we
report
our
observations
regarding
development
analyze
responses
to
mechanical
water
jet
stimuli.
Sensing
fish
head
may
be
crucial
avoid
obstacles,
catch
prey,
orient
current,
absence
visual
cues.
Alongside
trigeminal
system,
fine
nerve
endings
innervating
skin,
could
contribute
perceiving
mechanosensory
stimulation.
Therefore,
compare
responsiveness
auditory
neurons.
We
show
tuned
velocity
ramp
stimulation,
while
either
only
respond
step
stimuli
or
fast
Auditory
did
not
These
results
prove
essential
designing
underwater
robots
artificial
lines,
respect
spectra
systems
larval
to,
underline
importance
functionality
head.
Язык: Английский
Visualization of Glutamatergic Neurotransmission in Diverse Model Organisms with Genetically Encoded Indicators
Neuromethods,
Год журнала:
2024,
Номер
unknown, С. 3 - 34
Опубликована: Янв. 1, 2024
Glutamate
is
the
principal
excitatory
neurotransmitter,
and
occasionally
subserves
inhibitory
roles,
in
vertebrate
nervous
system.
Glutamatergic
synapses
are
dense
brain,
at
~1/μm3.
released
from
onto
diverse
components
of
system,
including
neurons,
glia,
other
cells.
Methods
for
glutamate
detection
critically
important
understanding
function
neural
circuits
normal
physiology,
development,
disease.
Here
we
describe
optimization,
deployment
genetically
encoded
fluorescent
indicators.
We
review
theoretical
considerations
governing
sensor
properties
first
principles
synapse
biology,
microscopy,
protein
structure-function
relationships.
provide
case
studies
state-of-the-art
iGluSnFR
sensor,
encompassing
design
mechanism
action,
vivo
imaging,
data
analysis,
future
directions.
include
detailed
protocols
imaging
common
preparations
(bacteria,
cell
culture,
brain
slices)
model
organisms
(worm,
fly,
fish,
rodent).
Язык: Английский
Different behaviourally relevant stimuli evoke different forms of adaptation in the olfactory system
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 15, 2024
Abstract
Sensory
neurons
are
continuously
exposed
to
a
large
diversity
of
stimuli
and
adjust
their
response
according
the
recent
stimulation
history,
in
process
called
adaptation.
Recent
studies
have
demonstrated
that
many
sensory
not
only
depress
(decrease
repetitive
stimulation)
but
some
exhibit
facilitation:
small
initial
followed
by
increase
amplitude.
Adaptation
has
been
mainly
studied
with
neutral
it
is
known
whether
different
behaviourally
relevant
evoke
adaptation
similar
or
properties.
Here
we
used
ethologically
study
zebrafish
olfactory
system.
We
found
presentation
food
odour
caused
variety
adaptations
ranging
from
very
strong
depression
facilitation,
was
predominant
dynamic.
On
other
hand,
behaviourally-relevant
stimulus,
alarm
substance,
evoked
much
stronger
facilitation
also
decreased
amplitude
responses
presentation.
Other
modalities,
aversive
mechanosensory
attractive
visual
stimuli,
properties
same
brain
area.
Different
forms
may
therefore
be
for
processing
evoking
behavioural
reactions.
Graphical
abstract
Highlights
Food
evokes
extreme
Alarm
substance
than
inhibits
Mechanosensory
both
Visual
bulbs
strongly
quickly
Язык: Английский