Adropin/Tirzepatide Combination Mitigates Cardiac Metabolic Aberrations in a Rat Model of Polycystic Ovarian Syndrome, Implicating the Role of the AKT/GSK3β/NF-κB/NLRP3 Pathway DOI Open Access
Islam Ibrahim Hegab, Hemat El‐Sayed El‐Horany, Rania Nagi Abd‐Ellatif

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 26(1), С. 1 - 1

Опубликована: Дек. 24, 2024

Polycystic ovarian syndrome (PCOS) is a multifaceted metabolic and hormonal disorder in females of reproductive age, frequently associated with cardiac disturbances. This research aimed to explore the protective potential adropin and/or tirzepatide (Tirze) on cardiometabolic aberrations letrozole-induced PCOS model. Female Wistar non-pregnant rats were allotted into five groups: CON; PCOS; + adropin; Tirze; adropin+ Tirze. The serum sex hormones, glucose, lipid profiles securitized. Cardiac phosphorylated levels AKT(pAKT), glycogen synthase kinase-3 beta (pGSK-3β), NOD-like receptor family pyrin domain containing 3 (NLPR3), IL-1β IL-18 assayed. redox status endoplasmic reticulum stress (ER) parameters including relative glucose-regulated protein 78 (GRP78) C/EBP homologous (CHOP) gene expressions detected. Finally, immunoreactivity NF-κB, Bcl2, BAX assessed. Our results displayed that Tirze intervention successfully alleviated PCOS-provoked derangements better recorded for combination treatment. synergistic effect mostly mediated via activating Akt, which dampens GSK3β/NF-κB/NLRP3 signaling pathway, sequel alleviating oxidative damage, inflammatory response, ER stress, related apoptosis, making them alluring desirable therapeutic targets PCOS-associated complications.

Язык: Английский

Gut Microbiota Modulation by Inulin Improves Metabolism and Ovarian Function in Polycystic Ovary Syndrome DOI Creative Commons
Lulu Geng, Xin Yang, Jiani Sun

и другие.

Advanced Science, Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

Abstract The management of metabolic disorder associated with polycystic ovary syndrome (PCOS) has been suggested as an effective approach to improve PCOS which is highly involved gut microbiota, while the underlying mechanism unclear. Here, we investigated role inulin, a microbiota regulator, in alleviation PCOS. Our findings showed that inulin treatment significantly improved hyperandrogenism and glucolipid metabolism both cohort mice. Consistent cohort, increased abundance microbial co‐abundance group (CAG) 12 mice, including Bifidobacterium species other short‐chain fatty acids (SCFAs)‐producers. We further verified enhancement SCFAs biosynthesis capacity fecal content by inulin. Moreover, decreased lipopolysaccharide‐binding protein (LBP) ameliorated ovarian inflammation whereas intraperitoneal lipopolysaccharide (LPS) administration reversed protective effects Furthermore, transplantation (FMT) from inulin‐treated patients enhanced insulin sensitivity, lipid accumulation thermogenesis, reduced inflammatory response antibiotic‐treated Collectively, these revealed mediates beneficial on dysfunction Therefore, modulating represents promising therapeutic strategy for

Язык: Английский

Процитировано

0
Quinoa ameliorates polycystic ovary syndrome via regulating gut microbiota through PI3K/AKT/mTOR pathway and autophagy DOI Creative Commons

Jinfang Dou,

Yanxiang Wu, Rentong Hu

и другие.

Nutrition & Metabolism, Год журнала: 2024, Номер 21(1)

Опубликована: Окт. 11, 2024

Polycystic ovary syndrome (PCOS) is a unity of endocrine and metabolic disorders, associated with PI3K/AKT/mTOR, autophagy, gut microbiota. Quinoa valuable food source, which contains rich minerals, unsaturated fatty acids, has positive modulating effect on diseases. However, its effects potential mechanisms PCOS have not been reported yet. Therefore, the purpose this study to investigate quinoa rats by regulating Ten–week-old female Sprague-Dawley (SD) received letrozole for 24 days induction subsequently were treated diet 8 weeks. Vaginal smears used analyze estrous cycle rats. Hormone biochemical indexes analyzed kit assays glucometer. The pathological changes ovary, pancreas, duodenum colon observed HE staining. PI3K, AKT, mTOR autophagy-related proteins in measured western blot immunohistochemistry Tight junction 16 s rDNA sequencing was detect intestinal microbiota Network pharmacology molecular docking possible targets PCOS. Spearman correlation analysis relationship between microbial abundance hormone levels at phylum genus level. significantly improved parameters PCOS-like rats, state tissues. Especially, regulated expression ovary. may repair barrier upregulating tight colon, regulate factors colon. Additionally, increased Lactobacillu, Bacteroides Oscillospira, decreased Firmicutes/Bacteroidetes ratio Blautia, Prevotella, reversing dysregulation Correlation showed that there strong significant related Our result indicated maybe activation PI3K/AKT/mTOR signaling pathway, inhibition regulation flora.

Язык: Английский

Процитировано

0
Adropin/Tirzepatide Combination Mitigates Cardiac Metabolic Aberrations in a Rat Model of Polycystic Ovarian Syndrome, Implicating the Role of the AKT/GSK3β/NF-κB/NLRP3 Pathway DOI Open Access
Islam Ibrahim Hegab, Hemat El‐Sayed El‐Horany, Rania Nagi Abd‐Ellatif

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 26(1), С. 1 - 1

Опубликована: Дек. 24, 2024

Polycystic ovarian syndrome (PCOS) is a multifaceted metabolic and hormonal disorder in females of reproductive age, frequently associated with cardiac disturbances. This research aimed to explore the protective potential adropin and/or tirzepatide (Tirze) on cardiometabolic aberrations letrozole-induced PCOS model. Female Wistar non-pregnant rats were allotted into five groups: CON; PCOS; + adropin; Tirze; adropin+ Tirze. The serum sex hormones, glucose, lipid profiles securitized. Cardiac phosphorylated levels AKT(pAKT), glycogen synthase kinase-3 beta (pGSK-3β), NOD-like receptor family pyrin domain containing 3 (NLPR3), IL-1β IL-18 assayed. redox status endoplasmic reticulum stress (ER) parameters including relative glucose-regulated protein 78 (GRP78) C/EBP homologous (CHOP) gene expressions detected. Finally, immunoreactivity NF-κB, Bcl2, BAX assessed. Our results displayed that Tirze intervention successfully alleviated PCOS-provoked derangements better recorded for combination treatment. synergistic effect mostly mediated via activating Akt, which dampens GSK3β/NF-κB/NLRP3 signaling pathway, sequel alleviating oxidative damage, inflammatory response, ER stress, related apoptosis, making them alluring desirable therapeutic targets PCOS-associated complications.

Язык: Английский

Процитировано

0