Fluorescence Lifetime Imaging Techniques—A Review on Principles, Applications and Clinical Relevance DOI Creative Commons
Vladislav I. Shcheslavskiy, Marina V. Shirmanova, Konstantin Yashin

и другие.

Journal of Biophotonics, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

ABSTRACT This article gives an overview of the most frequently used fluorescence‐lifetime imaging (FLIM) techniques, their capabilities, and typical applications. Starting from a general introduction to fluorescence phosphorescence lifetime, we will show that lifetime or, more accurately, decay function fluorophore is direct indicator interaction with its molecular environment. FLIM therefore than simple contrast technique in microscopy—it imaging. techniques can be classified into time‐domain frequency‐domain analogue photon counting scanning wide‐field techniques. these technical principles describe features peculiarities different use. An extended section dedicated TCSPC FLIM, addressing unique capabilities make especially interesting biological systems.

Язык: Английский

Fluorescence Lifetime Imaging Techniques—A Review on Principles, Applications and Clinical Relevance DOI Creative Commons
Vladislav I. Shcheslavskiy, Marina V. Shirmanova, Konstantin Yashin

и другие.

Journal of Biophotonics, Год журнала: 2025, Номер unknown

Опубликована: Фев. 19, 2025

ABSTRACT This article gives an overview of the most frequently used fluorescence‐lifetime imaging (FLIM) techniques, their capabilities, and typical applications. Starting from a general introduction to fluorescence phosphorescence lifetime, we will show that lifetime or, more accurately, decay function fluorophore is direct indicator interaction with its molecular environment. FLIM therefore than simple contrast technique in microscopy—it imaging. techniques can be classified into time‐domain frequency‐domain analogue photon counting scanning wide‐field techniques. these technical principles describe features peculiarities different use. An extended section dedicated TCSPC FLIM, addressing unique capabilities make especially interesting biological systems.

Язык: Английский

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