Laser & Photonics Review, Год журнала: 2024, Номер 18(10)

Опубликована: Июль 3, 2024

Abstract Irreproducibility in molecular optical sectioning microscopy has hindered the transformation of acquired digital images from qualitative descriptions to quantitative data. Although numerous tools, metrics, and phantoms have been developed, accurate comparisons data different systems with diverse acquisition conditions remains a challenge. Here, they develop simple tool based on an absolute measurement bulk fluorophore solutions related Poisson photon statistics, overcome this obstacle is developed. Demonstrated prototypical multiphoton microscope, unifies unit pixelated enable objective comparison imaging performance across modalities, microscopes, components/settings, targets. The application live specimens identifies attractive methodology for imaging, which rapidly acquires low signal‐to‐noise frames either gentle illumination or low‐concentration fluorescence labeling.

Язык: Английский