Endogenous Opioid Dynamics in the Dorsal Striatum Sculpt Neural Activity to Control Goal-Directed Action DOI
Raajaram Gowrishankar,

Madelyn M. Gray,

Abigail J. Elerding

и другие.

Опубликована: Янв. 1, 2024

Endogenous neuropeptides are uniquely poised to regulate neuronal activity and behavior across multiple timescales. Traditional studies ascribing neuropeptide contributions lack spatiotemporal precision. The endogenous opioid dynorphin is highly enriched in the dorsal striatum, known be critical for regulating goal-directed behavior. However, locus, precise timescale, or functional role of dyn-KOR signaling on unknown. Here, we report that local, time-locked release from dorsomedial striatum necessary sufficient using a suite high resolution modern approaches including vivo two-photon imaging, biosensor detection, conditional deletions optogenetic manipulations. We discovered glutamatergic axon terminals basolateral amygdala evoke striatal release, resulting retrograde presynaptic GPCR inhibition during Collectively, our findings isolate causal at rapid timescales, subsequent tuning promoting fundamental behaviors.

Язык: Английский

Illuminating the impact of stress: in vivo approaches to track stress-related neural adaptations. DOI Creative Commons
Puja K. Parekh

Neurobiology of Stress, Год журнала: 2025, Номер 35, С. 100712 - 100712

Опубликована: Фев. 7, 2025

Stressful experiences can affect both daily life and long-term health outcomes in a variety of ways. Acute challenges may be adaptive, promoting arousal enhancing memory cognitive function. Importantly, however, chronic stress dysregulates the body's physiological regulatory mechanisms consisting complex hormone interactions throughout peripheral central nervous systems. This disrupted signaling consequently alters balance synapse formation, maturation pruning, processes which regulate neural communication, plasticity, learning, flexibility adaptive behaviors - hallmarks healthy, functional brain. The chronically stressed brain state, therefore, is one uniquely vulnerable. To understand development this how it sustained behavior function are transiently or indelibly impacted by it, we turn to number advanced approaches animal models offer unprecedented insights. has been aim my recent work within field goal new independent research program. achieve this, I have employed methods uncover key circuits integrate information support motivated behaviors, impacts their ability perform process best operationalize behavioral readouts. Here present an overview contributions that find most meaningful for advancing our understanding impact propose avenues will guide own framework address salient outstanding questions field.

Язык: Английский

Процитировано

0
Multiphoton Neurophotonics: Recent Advances in Imaging and Manipulating Neuronal Circuits DOI
Cécile Telliez, Ruth R. Sims, Giulia Faini

и другие.

ACS Photonics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

Язык: Английский

Процитировано

0
Endogenous opioid dynamics in the dorsal striatum sculpt neural activity to control goal-directed action DOI Creative Commons
Raajaram Gowrishankar, Madelyn M. Hjort,

Abigail J. Elerding

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Май 20, 2024

Abstract Endogenous neuropeptides are uniquely poised to regulate neuronal activity and behavior across multiple timescales. Traditional studies ascribing neuropeptide contributions lack spatiotemporal precision. The endogenous opioid dynorphin is highly enriched in the dorsal striatum, known be critical for regulating goal-directed behavior. However, locus, precise timescale, or functional role of dyn-KOR signaling on unknown. Here, we report that local, time-locked release from dorsomedial striatum necessary sufficient using a suite high resolution modern approaches including vivo two-photon imaging, biosensor detection, conditional deletions optogenetic manipulations. We discovered glutamatergic axon terminals basolateral amygdala evoke striatal release, resulting retrograde presynaptic GPCR inhibition during Collectively, our findings isolate causal at rapid timescales, subsequent tuning promoting fundamental behaviors.

Язык: Английский

Процитировано

3
Endogenous Opioid Dynamics in the Dorsal Striatum Sculpt Neural Activity to Control Goal-Directed Action DOI
Raajaram Gowrishankar,

Madelyn M. Gray,

Abigail J. Elerding

и другие.

Опубликована: Янв. 1, 2024

Endogenous neuropeptides are uniquely poised to regulate neuronal activity and behavior across multiple timescales. Traditional studies ascribing neuropeptide contributions lack spatiotemporal precision. The endogenous opioid dynorphin is highly enriched in the dorsal striatum, known be critical for regulating goal-directed behavior. However, locus, precise timescale, or functional role of dyn-KOR signaling on unknown. Here, we report that local, time-locked release from dorsomedial striatum necessary sufficient using a suite high resolution modern approaches including vivo two-photon imaging, biosensor detection, conditional deletions optogenetic manipulations. We discovered glutamatergic axon terminals basolateral amygdala evoke striatal release, resulting retrograde presynaptic GPCR inhibition during Collectively, our findings isolate causal at rapid timescales, subsequent tuning promoting fundamental behaviors.

Язык: Английский

Процитировано

0