AG1®, a Novel Synbiotic, Maintains Gut Barrier Function following Inflammatory Challenge in a Caco-2/THP1-Blue™ Co-Culture Model

Microorganisms, Год журнала: 2024, Номер 12(7), С. 1263 - 1263

Опубликована: Июнь 21, 2024

Nutritional interventions to reduce gastrointestinal (GI) permeability are of significant interest physically active adults and those experiencing chronic health conditions. This in vitro study was designed assess the impact AG1, a novel synbiotic, on GI following an inflammatory challenge. Interventions [AG1 (vitamins/minerals, pre-/probiotics, phytonutrients) control (control medium)] were fed separately into human tract model (stomach, small intestine, colon). In colonic phase, contents combined with fecal inocula from three healthy donors. evaluated transepithelial electrical resistance (TEER) Caco-2 (apical)/THP1-Blue™ (basolateral) co-culture model. The apical side received sodium butyrate (positive control) or complete medium (negative during baseline testing. 24 h experiment, simulation isolates model, basolateral treated medium, then 6 treatment lipopolysaccharide. TEER assessed at 0 h, markers measured 30 triplicate. Paired samples

Язык: Английский

---

The Influence of Dietary Supplements on Exercise-Induced Gut Damage and Gastrointestinal Symptoms: A Systematic Review and Meta-Analysis

Nutrients, Год журнала: 2025, Номер 17(3), С. 443 - 443

Опубликована: Янв. 25, 2025

Endurance exercise, especially under heat stress, temporarily compromises the integrity of intestinal barrier in healthy individuals. Consequently, there is growing interest developing effective dietary strategies to alleviate exercise-induced gastrointestinal symptoms and gut damage. This meta-analysis investigated effects supplements on mitigating these challenges. The search was performed November 2024 following PRISMA guidelines, 26 peer-reviewed studies were included across three meta-analyses: (1) symptoms, (2) circulating fatty acid-binding protein (i-FABP), (3) exercise performance. moderating effect variables assessed via sub-group analysis meta-regression. Overall, no pooled supplement interventions (Hedges’ g = 0.42, 95% CI −0.17: 1.02, p 0.15), probiotics had a moderate significant for −0.62, −1.01; 1.01, 0.05). There increase i-FABP concentrations pre- post (∆ 106%; Hedges’ 0.63; 1.38, 0.01). or differences performance any (p 0.53). Moderate-to-large heterogeneity observed (I2 ≥ 58.6%), candidate moderators (exercise duration, modality, environmental temperature) outcomes > A during observed. However, when examining different categories, although significance select few supplements, changes i-FABP, outside clinical relevance. Although showed conflicting findings may have been due inadequate control confounding studies. Further research required assess alternative supplements’ health performance, particularly varied conditions, where more rigorous cofounding factors implemented.

Язык: Английский

---

Anthocyanin-rich New Zealand blackcurrant extract reduces treadmill running-induced gastrointestinal symptoms in the heat: pilot observations

Research in Sports Medicine, Год журнала: 2025, Номер unknown, С. 1 - 8

Опубликована: Фев. 25, 2025

We examined the effect of anthocyanin-rich New Zealand blackcurrant (NZBC) extract on running-induced gastrointestinal symptoms in heat. Recreationally active men (n = 12, age: 28 ± 6 yr, BMI: 24.5 1.8 kg·m-2, V˙O2max: 56 mL·kg-1·min-1) volunteered. Participants dosed with 7 days NZBC (CurraNZ®) (210 mg anthocyanins) or placebo. Exercise consisted treadmill running for one hr at 65%V˙O2max (34.1 0.1°C, 40.8 0.2% relative humidity) recording during and 60 min following recovery normal conditions. With placebo, 11 participants (92%) reported (e.g. belching, stitch), this was reduced to four (25%) extract. Using a modified visual analog scale, accumulated score lower 7-day intake (placebo: 112, extract: 54, p 0.04). The prevalence upper, other (upper, placebo: 75%, 25%; lower: 25%, 17%; other, 50%, 25%). only two severe (one participant dizziness nausea that still reporting those condition). Seven-day incidence 1-h hot environmental Future research should examine efficacy exercise-associated conditions which can be expected severe.

Язык: Английский

---

Case Series: Management of Exercise-Associated Gastrointestinal Symptoms in Endurance Athletes Using a High-Carbohydrate Low-FODMAP Therapeutic Intervention

International Journal of Sport Nutrition and Exercise Metabolism, Год журнала: 2025, Номер unknown, С. 1 - 18

Опубликована: Янв. 1, 2025

This case-series describes a four-phase management approach implementing gastrointestinal assessment during exercise (GastroAxEx) to develop therapeutic interventions for athletes affected by exercise-associated symptoms (Ex-GIS). Recreational elite-level who experienced recurrent severe Ex-GIS (n = 9) and controls 2) (without Ex-GIS) volunteered participate in the case series. The four-phased involved clinical (Phase 1), standardized laboratory GastroAxEx where physiological strain GIS data were collected 2), individualized intervention strategies based on Phase 2 outcomes that specifically included implementation of 48-hr high carbohydrate low FODMAP diet 3), monitoring adjustment 4). majority 6/9) consumed <400 ml/hr fluid ad libitum, resulting 2%-4% body mass loss, <40 g/hr exogenous 8/9), GastroAxEx. Severe (predominantly loose stools) was reported n 4/9 athletes, whereas 8/9 recovery period. All food intake tolerance recovery. Orocecal transit time slow or delayed 2/9 athletes. Most 8/9) successfully implemented prior improved targeted event. In addition, incidence severity reduced, perceived work output events. A before endurance appears be valuable addition suggested used mitigate Ex-GIS.

Язык: Английский

---

Effect of 2000-meter rowing test on parameters of intestinal integrity in elite rowers during competitive phase - observational study

BMC Sports Science Medicine and Rehabilitation, Год журнала: 2025, Номер 17(1)

Опубликована: Март 18, 2025

The epithelial wall leakage has been extensively studied in sports disciplines like running and cycling. However, little is known about gut permeability other disciplines, rowing, especially after the regular competition performance distance of 2000 m. Therefore, our study aimed to check 2000-meter rowing test annual training cycle. phenomenon subject investigations within athletic domains such as Nevertheless, there exists an insufficiency understanding regarding alternative particularly following completion a standard competitive Hence, principal objective was assess test. performed at beginning phase. Eighteen elite rowers Polish Rowing Team participated applying inclusion/exclusion criteria. participants ergometer Blood samples were taken before test, exercise, 1-hour restitution. Parameters, I-FABP, LPS, LBP, zonulin, determined using appropriate biochemical tests. There no changes between pre- post-exercise values zonulin. I-FABP changed from 6,49 ± 2,15 8,3 2,71 (ng/ml) during recovery period, LBP decreased 2,73 0,77 2,035 0,53 (µg/ml) simultaneously. Other parameters have not changed. results this showed that intense physical effort period sufficient negatively affect integrity rowers.

Язык: Английский

---

Sports Dietitians Australia and Ultra Sports Science Foundation Joint Position Statement: A Practitioner Guide to the Prevention and Management of Exercise-Associated Gastrointestinal Perturbations and Symptoms

Sports Medicine, Год журнала: 2025, Номер unknown

Опубликована: Апрель 7, 2025

Язык: Английский

---

Feeding Tolerance to a Running Gut-Challenge Protocol: a Cross-Sectional Exploration

International Journal of Sports Medicine, Год журнала: 2025, Номер unknown

Опубликована: Апрель 27, 2025

Abstract This study examined gastrointestinal function, substrate utilization, and exercise performance differences in response to a gut-challenge between (1) female male athletes, (2) young adult versus masters (3) athletes with or without exercise-associated symptom history (Ex-GIS). Fifty-three endurance (43 males 10 females) completed involving 2-hour run (60% V̇O2max) 90 g/h carbohydrate intake, followed by 1-hour self-paced distance test (DT) ad libitum water. Blood breath samples were collected pre- post-exercise. glucose utilization during measured, while Ex-GIS feeding tolerance assessed pre-, during, Interest food appetite was lower symptomatic than asymptomatic (p<0.05). Females had higher blood concentration (p=0.037) immediately after (p=0.003) whole-body fat oxidation rate (p=0.009). Exercise did not differ based on the biological sex, age, history. Overall, functional markers comparable groups, availability appear be influenced respectively. Tailored management of issues should an individual basis as opposed general demographic characteristics.

Язык: Английский

---

Effect of prebiotics, probiotics, and synbiotics on gastrointestinal outcomes in healthy adults and active adults at rest and in response to exercise—A systematic literature review

Frontiers in Nutrition, Год журнала: 2022, Номер 9

Опубликована: Дек. 7, 2022

A systematic literature search was undertaken to assess the impact of pre-, pro-, and syn-biotic supplementation on measures gastrointestinal status at rest in response acute exercise.Six databases (Ovid MEDLINE, EMBASE, Cinahl, SportsDISCUS, Web Science, Scopus) were used. Included human research studies healthy sedentary adults, active involving control or placebo groups. Sedentary individuals with non-communicable disease risk established inflammatory functional diseases/disorders excluded.A total n = 1,204 participants included from 37 papers reported resting outcomes, 13 exercise-induced syndrome (EIGS) outcomes. No supplement improved permeability symptoms (GIS), systemic endotoxemia rest. Only modest positive changes cytokine profiles observed 3/15 Prebiotic (n 4/5) significantly increased fecal Bifidobacteria, but no consistent differences other microbes. Probiotic 4/9) supplemented bacterial species-strain. arabinoxylan oligosaccharide short chain fatty acid (SCFA) butyrate concentrations. In exercise, probiotics did not substantially influence epithelial injury permeability, endotoxin profile, GIS. Two reduced responses exercise. GIS during exercise.Synbiotic outcomes resembled probiotics, likely due minimal dose prebiotic included. Methodological issues high bias identified several studies, using Cochrane Risk Bias Assessment Tool. major limitation majority lack a comprehensive approach well-validated biomarkers specific many featured small sample sizes. can gut microbial composition SCFA concentration; whereas increase species-strain, effect SCFA, any marker synbiotic does reduce profiles,

Язык: Английский

---

Changes in surrogate markers of intestinal epithelial injury and microbial translocation in young and older men during prolonged occupational heat stress in temperate and hot conditions

European Journal of Applied Physiology, Год журнала: 2023, Номер 124(4), С. 1049 - 1062

Опубликована: Окт. 10, 2023

Язык: Английский

---

Exertional heat stress promotes the presence of bacterial DNA in plasma: A counterbalanced randomised controlled trial

Journal of science and medicine in sport, Год журнала: 2024, Номер 27(9), С. 610 - 617

Опубликована: Сен. 1, 2024

ObjectivesThe primary aim was to explore the impact of exertional-heat stress (EHS) promoted exercise-associated bacteraemia. A secondary examine if an amino acid beverage (AAB) intervention may mitigate bacteraemia.DesignCounterbalanced randomised control trial.MethodsTwenty endurance trained male participants completed two EHS trials. On one occasion, consumed a 237 mL AAB twice daily for 7-days prior, immediately before and every 20 min during (2 h running at 60 % V̇O2max in 35 °C). other water volume (CON) equivalent consumed. Whole blood samples were collected pre- post-EHS, analysed plasma DNA concentration by fluorometer quantification after microbial extraction, bacterial relative abundance next generation 16 s rRNA gene sequencing.ResultsIncreased post-EHS observed on CON (pre-EHS 0.014 ng/μL, 0.039 ng/μL) (p < 0.001) 0.015 0.031 0.001). The magnitude change from post-exercise 40 lower, but no significant difference versus = 0.455). Predominant groups identified included: phyla-Proteobacteria (88.0 %), family-Burkholderiaceae (59.1 genus-Curvibacter (58.6 %). No variation absolute α-diversity phyla, family, genus CON.ConclusionThe increased presence microbial-bacterial systemic circulation response appears positive all participants. An supplementation period prior consumption did not provide attenuation EHS-associated

Язык: Английский

---