Over-calcified forms of the coccolithophore <i>Emiliania huxleyi</i> in high-CO<sub>2</sub> waters are not preadapted to ocean acidification

Biogeosciences, Год журнала: 2018, Номер 15(5), С. 1515 - 1534

Опубликована: Март 14, 2018

Abstract. Marine multicellular organisms inhabiting waters with natural high fluctuations in pH appear more tolerant to acidification than conspecifics occurring nearby stable waters, suggesting that environments of fluctuating hold genetic reservoirs for adaptation key groups ocean (OA). The abundant and cosmopolitan calcifying phytoplankton Emiliania huxleyi exhibits a range morphotypes varying degrees coccolith mineralization. We show E. populations the naturally acidified upwelling eastern South Pacific, where drops below 7.8 as is predicted global surface by year 2100, are dominated exceptionally over-calcified whose distal shield can be almost solid calcite. Shifts morphotype composition correlate changes carbonate system parameters. tested if these correlations indicate hyper-calcified adapted OA. In experimental exposures present-day vs. future pCO2 (400 1200 µatm), showed same growth inhibition (−29.1±6.3 %) moderately calcified isolated from non-acidified water (−30.7±8.8 %). Under high-CO2–low-pH condition, production rates particulate organic carbon (POC) increased, while inorganic (PIC) were maintained or decreased slightly (but not significantly), leading lowered PIC ∕ POC ratios all strains. There no consistent response intensity strain origin. condition affected morphology equally strongly strains compared High-CO2–low-pH conditions directly select over other morphotypes, but perhaps indirectly ecologically correlated factors. More generally, results suggest oceanic planktonic microorganisms, despite their rapid turnover large population sizes, do necessarily exhibit adaptations high-CO2 upwellings, this ubiquitous coccolithophore may near limit its capacity adapt ongoing acidification.

Язык: Английский

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Trait drift in microalgae and applications for strain improvement

Biotechnology Advances, Год журнала: 2022, Номер 60, С. 108034 - 108034

Опубликована: Сен. 9, 2022

Язык: Английский

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The evolution of trait correlations constrains phenotypic adaptation to high CO₂in a eukaryotic alga

Proceedings of the Royal Society B Biological Sciences, Год журнала: 2021, Номер 288(1953)

Опубликована: Июнь 16, 2021

Microbes form the base of food webs and drive biogeochemical cycling. Predicting effects microbial evolution on global elemental cycles remains a significant challenge due to sheer number interacting environmental trait combinations. Here, we present an approach for integrating multivariate data into predictive model evolution. We investigated outcome thousands possible adaptive walks parameterized using empirical from alga Chlamydomonas exposed high CO 2 . found that direction historical bias (existing correlations) influenced both rate adaptation evolved phenotypes (trait combinations). Critically, use fitness landscapes derived directly values capture known evolutionary phenomena. This work demonstrates ecological models need represent changes in traits correlation between order accurately phytoplankton predict future shifts

Язык: Английский

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Local adaptation of a marine diatom is governed by genome-wide changes in diverse metabolic processes

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Сен. 23, 2023

ABSTRACT Marine phytoplankton play essential roles in global primary production and biogeochemical cycles. Yet, the evolutionary genetic underpinnings of adaptation to complex marine coastal environments, where many environmental variables fluctuate interact, remain unclear. We combined population genomics data with experimental transcriptomics investigate genomic basis underlying a natural experiment that has played out over past 8,000 years one world’s largest brackish water bodies: colonization Baltic Sea by diatom Skeletonema marinoi . To this end, we used novel approach for protist genomics, combining target capture entire nuclear genome pooled sequencing, showed method performs well on both cultures single cells. Genotype-environment association analyses identified >3,000 genes signals selection response major gradients Sea, which apart from salinity, include marked differences temperature nutrient availability. Locally adapted were related diverse metabolic processes, including signal transduction, cell cycle, DNA methylation, maintenance homeostasis. The locally significant overlap salinity-responsive laboratory common garden experiment, suggesting salinity gradient is factor driving local S. Altogether, our show are characterized multitude gradients, driven intricate changes pathways functions.

Язык: Английский

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Long-Term m5C Methylome Dynamics Parallel Phenotypic Adaptation in the CyanobacteriumTrichodesmium

Molecular Biology and Evolution, Год журнала: 2020, Номер 38(3), С. 927 - 939

Опубликована: Окт. 3, 2020

Abstract A major challenge in modern biology is understanding how the effects of short-term biological responses influence long-term evolutionary adaptation, defined as a genetically determined increase fitness to novel environments. This particularly important globally microbes experiencing rapid global change, due their on food webs, biogeochemical cycles, and climate. Epigenetic modifications like methylation have been demonstrated plastic responses, which ultimately impact adaptive environmental change. However, there remains paucity empirical research examining dynamics during adaptation nonmodel, ecologically microbes. Here, we show first evidence marine prokaryote for m5C methylome correlated with phenotypic CO2, using 7-year evolution experiment (1,000+ generations) biogeochemically cyanobacterium Trichodesmium. We identify methylated sites that rapidly changed response high (750 µatm) CO2 exposure were maintained at least 4.5 years selection. After 7 selection, however, levels initially responded high-CO2 returned ancestral, ambient levels. Concurrently, adapted growth N2 fixation rates remained significantly higher than those cell lines irrespective concentration, trend consistent genetic assimilation theory. These data demonstrate maintenance CO2-responsive alongside before returning ancestral observations distributed provide critical insights into traits under

Язык: Английский

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