Tunable Polymeric Scaffolds for Enzyme Immobilization DOI Creative Commons
Andoni Rodriguez‐Abetxuko, Daniel Sánchez‐deAlcázar,

Pablo Muñumer

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2020, Номер 8

Опубликована: Июль 30, 2020

The number of methodologies for the immobilization enzymes using polymeric supports is continuously growing due to developments in fields biotechnology, polymer chemistry, and nanotechnology last years. Despite being excellent catalysts, are very sensitive molecules can undergo denaturation beyond their natural environment. For overcoming this issue, chemistry offers a wealth opportunities successful combination with versatile or synthetic polymers. fabrication functional, stable, robust biocatalytic hybrid materials (nanoparticles, capsules, hydrogels, films) has been proven advantageous several applications such as biomedicine, organic synthesis, biosensing, bioremediation. In review, supported recent examples enzyme-protein hybrids, we provide an overview methods used combine both macromolecules, well future directions main challenges that currently tackled field.

Язык: Английский

Insight of MOF Environment-Dependent Enzyme Activity via MOFs-in-Nanochannels Configuration DOI

Junli Guo,

Lingling Yang, Zhida Gao

и другие.

ACS Catalysis, Год журнала: 2020, Номер 10(10), С. 5949 - 5958

Опубликована: Май 6, 2020

Maintaining the high activity of an enzyme is a fundamental requirement to widen application metal–organic frameworks (MOFs) in biotechnology, biosensor, and biomedicine fields. However, it still challenging monitor understand MOF environment-related for enzyme. Here, we developed MOFs-in-nanochannels configuration broadening biocatalytic MOFs on demand. ZIF-8 [Zn(mim)2, Hmim = 2-methylimidazolated] grown TiO2 nanochannels used as platform, cytochrome C (CytC) model encapsulated ZIF-8. The enzymatic catalytic process converts 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) positively charged product (ABTS+). On basis current–voltage properties, change ion transport characteristics can be monitored with time. encapsulating CytC molecules not only exhibit significantly enhanced wide temperature region (37–80 °C) but also have remarkably long storage stability at room temperature. results quantum mechanical calculation indicate that Fe–S bond inclined break environment owing confinement effect structure, favorable catalysis. MOFs-in-nanochannel provides innovative label-free design onsite monitoring MOFs, which holds great potential constructing biosensing platforms remarkable performance stability.

Язык: Английский

Процитировано

124

Recent Trends in Enzyme Immobilization—Concepts for Expanding the Biocatalysis Toolbox DOI Creative Commons
Hans‐Jürgen Federsel, Thomas S. Moody, Stephen Taylor

и другие.

Molecules, Год журнала: 2021, Номер 26(9), С. 2822 - 2822

Опубликована: Май 10, 2021

Enzymes have been exploited by humans for thousands of years in brewing and baking, but it is only recently that biocatalysis has become a mainstream technology synthesis. Today, enzymes are used extensively the manufacturing pharmaceuticals, food, fine chemicals, flavors, fragrances other products. Enzyme immobilization also developed parallel as means increasing enzyme performance reducing process costs. The aim this review to present discuss some more recent promising technical developments immobilization, including supports used, methods fabrication, their application highlights new support technologies such use well-established polysaccharides novel ways, magnetic particles, DNA, renewable materials hybrid organic–inorganic supports. addresses how being integrated into developing biocatalytic technology, example flow biocatalysis, 3D printing multi-enzymatic cascade reactions.

Язык: Английский

Процитировано

103

Insights into the Enhanced Catalytic Activity of Cytochrome c When Encapsulated in a Metal–Organic Framework DOI
Yijing Chen, Felipe Jiménez‐Ángeles, Baofu Qiao

и другие.

Journal of the American Chemical Society, Год журнала: 2020, Номер 142(43), С. 18576 - 18582

Опубликована: Окт. 13, 2020

The encapsulation of enzymes within porous materials has shown great promise, not only in protecting the from denaturation under nonbiological environments, but also, some cases, facilitating their enzymatic reaction rates at favorable conditions. While a number hypotheses have been developed to explain this phenomenon, detailed structural changes upon material, which are closely related activity, remain largely elusive. Herein, change cytochrome c (Cyt c) hierarchical metal-organic framework, NU-1000, is investigated through combination experimental and computational methods, such as electron paramagnetic resonance, solid-state ultraviolet-visible spectroscopy, all-atom explicit solvent molecular dynamics simulations. enhanced catalytic performance Cyt after being encapsulated NU-1000 supported by physical silico observations around heme ferric active center.

Язык: Английский

Процитировано

100

Hierarchical micro- and mesoporous ZIF-8 with core–shell superstructures using colloidal metal sulfates as soft templates for enzyme immobilization DOI

Yuxiao Feng,

Yingjie Du,

Geling Kuang

и другие.

Journal of Colloid and Interface Science, Год журнала: 2021, Номер 610, С. 709 - 718

Опубликована: Ноя. 25, 2021

Язык: Английский

Процитировано

98

Tunable Polymeric Scaffolds for Enzyme Immobilization DOI Creative Commons
Andoni Rodriguez‐Abetxuko, Daniel Sánchez‐deAlcázar,

Pablo Muñumer

и другие.

Frontiers in Bioengineering and Biotechnology, Год журнала: 2020, Номер 8

Опубликована: Июль 30, 2020

The number of methodologies for the immobilization enzymes using polymeric supports is continuously growing due to developments in fields biotechnology, polymer chemistry, and nanotechnology last years. Despite being excellent catalysts, are very sensitive molecules can undergo denaturation beyond their natural environment. For overcoming this issue, chemistry offers a wealth opportunities successful combination with versatile or synthetic polymers. fabrication functional, stable, robust biocatalytic hybrid materials (nanoparticles, capsules, hydrogels, films) has been proven advantageous several applications such as biomedicine, organic synthesis, biosensing, bioremediation. In review, supported recent examples enzyme-protein hybrids, we provide an overview methods used combine both macromolecules, well future directions main challenges that currently tackled field.

Язык: Английский

Процитировано

95