Frontiers in Bioengineering and Biotechnology,
Год журнала:
2020,
Номер
8
Опубликована: Июль 30, 2020
The
number
of
methodologies
for
the
immobilization
enzymes
using
polymeric
supports
is
continuously
growing
due
to
developments
in
fields
biotechnology,
polymer
chemistry,
and
nanotechnology
last
years.
Despite
being
excellent
catalysts,
are
very
sensitive
molecules
can
undergo
denaturation
beyond
their
natural
environment.
For
overcoming
this
issue,
chemistry
offers
a
wealth
opportunities
successful
combination
with
versatile
or
synthetic
polymers.
fabrication
functional,
stable,
robust
biocatalytic
hybrid
materials
(nanoparticles,
capsules,
hydrogels,
films)
has
been
proven
advantageous
several
applications
such
as
biomedicine,
organic
synthesis,
biosensing,
bioremediation.
In
review,
supported
recent
examples
enzyme-protein
hybrids,
we
provide
an
overview
methods
used
combine
both
macromolecules,
well
future
directions
main
challenges
that
currently
tackled
field.
ACS Catalysis,
Год журнала:
2020,
Номер
10(10), С. 5949 - 5958
Опубликована: Май 6, 2020
Maintaining
the
high
activity
of
an
enzyme
is
a
fundamental
requirement
to
widen
application
metal–organic
frameworks
(MOFs)
in
biotechnology,
biosensor,
and
biomedicine
fields.
However,
it
still
challenging
monitor
understand
MOF
environment-related
for
enzyme.
Here,
we
developed
MOFs-in-nanochannels
configuration
broadening
biocatalytic
MOFs
on
demand.
ZIF-8
[Zn(mim)2,
Hmim
=
2-methylimidazolated]
grown
TiO2
nanochannels
used
as
platform,
cytochrome
C
(CytC)
model
encapsulated
ZIF-8.
The
enzymatic
catalytic
process
converts
2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate)
(ABTS)
positively
charged
product
(ABTS+).
On
basis
current–voltage
properties,
change
ion
transport
characteristics
can
be
monitored
with
time.
encapsulating
CytC
molecules
not
only
exhibit
significantly
enhanced
wide
temperature
region
(37–80
°C)
but
also
have
remarkably
long
storage
stability
at
room
temperature.
results
quantum
mechanical
calculation
indicate
that
Fe–S
bond
inclined
break
environment
owing
confinement
effect
structure,
favorable
catalysis.
MOFs-in-nanochannel
provides
innovative
label-free
design
onsite
monitoring
MOFs,
which
holds
great
potential
constructing
biosensing
platforms
remarkable
performance
stability.
Molecules,
Год журнала:
2021,
Номер
26(9), С. 2822 - 2822
Опубликована: Май 10, 2021
Enzymes
have
been
exploited
by
humans
for
thousands
of
years
in
brewing
and
baking,
but
it
is
only
recently
that
biocatalysis
has
become
a
mainstream
technology
synthesis.
Today,
enzymes
are
used
extensively
the
manufacturing
pharmaceuticals,
food,
fine
chemicals,
flavors,
fragrances
other
products.
Enzyme
immobilization
also
developed
parallel
as
means
increasing
enzyme
performance
reducing
process
costs.
The
aim
this
review
to
present
discuss
some
more
recent
promising
technical
developments
immobilization,
including
supports
used,
methods
fabrication,
their
application
highlights
new
support
technologies
such
use
well-established
polysaccharides
novel
ways,
magnetic
particles,
DNA,
renewable
materials
hybrid
organic–inorganic
supports.
addresses
how
being
integrated
into
developing
biocatalytic
technology,
example
flow
biocatalysis,
3D
printing
multi-enzymatic
cascade
reactions.
Journal of the American Chemical Society,
Год журнала:
2020,
Номер
142(43), С. 18576 - 18582
Опубликована: Окт. 13, 2020
The
encapsulation
of
enzymes
within
porous
materials
has
shown
great
promise,
not
only
in
protecting
the
from
denaturation
under
nonbiological
environments,
but
also,
some
cases,
facilitating
their
enzymatic
reaction
rates
at
favorable
conditions.
While
a
number
hypotheses
have
been
developed
to
explain
this
phenomenon,
detailed
structural
changes
upon
material,
which
are
closely
related
activity,
remain
largely
elusive.
Herein,
change
cytochrome
c
(Cyt
c)
hierarchical
metal-organic
framework,
NU-1000,
is
investigated
through
combination
experimental
and
computational
methods,
such
as
electron
paramagnetic
resonance,
solid-state
ultraviolet-visible
spectroscopy,
all-atom
explicit
solvent
molecular
dynamics
simulations.
enhanced
catalytic
performance
Cyt
after
being
encapsulated
NU-1000
supported
by
physical
silico
observations
around
heme
ferric
active
center.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2020,
Номер
8
Опубликована: Июль 30, 2020
The
number
of
methodologies
for
the
immobilization
enzymes
using
polymeric
supports
is
continuously
growing
due
to
developments
in
fields
biotechnology,
polymer
chemistry,
and
nanotechnology
last
years.
Despite
being
excellent
catalysts,
are
very
sensitive
molecules
can
undergo
denaturation
beyond
their
natural
environment.
For
overcoming
this
issue,
chemistry
offers
a
wealth
opportunities
successful
combination
with
versatile
or
synthetic
polymers.
fabrication
functional,
stable,
robust
biocatalytic
hybrid
materials
(nanoparticles,
capsules,
hydrogels,
films)
has
been
proven
advantageous
several
applications
such
as
biomedicine,
organic
synthesis,
biosensing,
bioremediation.
In
review,
supported
recent
examples
enzyme-protein
hybrids,
we
provide
an
overview
methods
used
combine
both
macromolecules,
well
future
directions
main
challenges
that
currently
tackled
field.
