Abstract
The
interplay
between
immune
cells/macrophages
and
fibroblast‐like
synoviocytes
(FLSs)
plays
a
pivotal
role
in
initiating
synovitis;
however,
their
involvement
metabolic
disorders,
including
diabetic
osteoarthritis
(DOA),
is
largely
unknown.
In
this
study,
single‐cell
RNA
sequencing
(scRNA‐seq)
employed
to
investigate
the
synovial
cell
composition
of
DOA.
A
significant
enrichment
activated
macrophages
within
eight
distinct
clusters
found
DOA
synovium.
Moreover,
it
demonstrated
that
increased
glycolysis
FLSs
key
driver
for
patients’
macrophage
infiltration
polarization.
addition,
yes‐associated
protein
1
(YAP1)/thioredoxin‐interacting
(TXNIP)
signaling
axis
play
crucial
regulating
glucose
transporter
(GLUT1)‐dependent
FLSs,
thereby
controlling
expression
series
adhesion
molecules
such
as
intercellular
molecule‐1
(ICAM‐1)
which
may
subsequently
fine‐tune
M1‐polarized
patients
db/db
OA
mice.
For
treatment,
M1
membrane‐camouflaged
Verteporfin
(Vt)‐loaded
PLGA
nanoparticles
(MVPs)
are
developed
ameliorate
progression
by
YAP1/TXNIP
axis,
thus
suppressing
macrophages.
results
provide
several
novel
insights
into
pathogenesis
offer
promising
treatment
approach
Military Medical Research,
Год журнала:
2024,
Номер
11(1)
Опубликована: Фев. 18, 2024
Abstract
Scar
formation
resulting
from
burns
or
severe
trauma
can
significantly
compromise
the
structural
integrity
of
skin
and
lead
to
permanent
loss
appendages,
ultimately
impairing
its
normal
physiological
function.
Accumulating
evidence
underscores
potential
targeted
modulation
mechanical
cues
enhance
regeneration,
promoting
scarless
repair
by
influencing
extracellular
microenvironment
driving
phenotypic
transitions.
The
field
appendage
regeneration
has
witnessed
remarkable
advancements
in
utilization
biomaterials
with
distinct
physical
properties.
However,
a
comprehensive
understanding
underlying
mechanisms
remains
somewhat
elusive,
limiting
broader
application
these
innovations.
In
this
review,
we
present
two
promising
biomaterial-based
approaches
aimed
at
bolstering
regenerative
capacity
compromised
skin.
first
approach
involves
leveraging
specific
biophysical
properties
create
an
optimal
environment
that
supports
cellular
activities
essential
for
regeneration.
second
centers
on
harnessing
forces
exerted
plasticity,
facilitating
efficient
reprogramming
and,
consequently,
appendages.
summary,
manipulation
using
strategies
holds
significant
promise
as
supplementary
achieving
wound
healing,
coupled
restoration
multiple
functions.
Experimental Hematology and Oncology,
Год журнала:
2025,
Номер
14(1)
Опубликована: Апрель 10, 2025
Abstract
Tumor
microenvironment
(TME)
is
a
complex
ecosystem
composed
of
both
cellular
and
non-cellular
components
that
surround
tumor
tissue.
The
extracellular
matrix
(ECM)
key
component
the
TME,
performing
multiple
essential
functions
by
providing
mechanical
support,
shaping
regulating
metabolism
signaling,
modulating
immune
responses,
all
which
profoundly
influence
cell
behavior.
quantity
cross-linking
status
stromal
are
primary
determinants
tissue
stiffness.
During
development,
ECM
stiffness
not
only
serves
as
barrier
to
hinder
drug
delivery
but
also
promotes
cancer
progression
inducing
stimulation
activates
membrane
receptors
sensors.
Thus,
comprehensive
understanding
how
regulates
crucial
for
identifying
potential
therapeutic
targets
cancer.
This
review
examines
effects
on
progression,
encompassing
proliferation,
migration,
metastasis,
resistance,
angiogenesis,
epithelial-mesenchymal
transition
(EMT),
evasion,
stemness,
metabolic
reprogramming,
genomic
stability.
Finally,
we
explore
strategies
target
their
implications
progression.
Biomaterials,
Год журнала:
2021,
Номер
279, С. 121190 - 121190
Опубликована: Окт. 21, 2021
Spinal
cord
injury
(SCI)
is
one
of
the
most
challenging
clinical
issues.
It
characterized
by
disruption
neural
circuitry
and
connectivity,
resulting
in
neurological
disability.
Adipose-derived
stem
cells
(ADSCs)
serve
as
a
promising
source
therapeutic
for
SCI
treatment.
However,
outcomes
direct
ADSCs
transplantation
are
limited
presence
an
inflammatory
microenvironment.
Herein,
cell-adaptable
neurogenic
(CaNeu)
hydrogel
was
developed
delivery
vehicle
to
promote
neuronal
regeneration
after
SCI.
The
dynamic
network
CaNeu
loaded
with
provides
cell-infiltratable
matrix
that
enhances
axonal
growth
eventually
leads
improved
motor
evoked
potential,
hindlimb
strength,
coordination
complete
spinal
transection
rats.
Furthermore,
also
establishes
anti-inflammatory
microenvironment
inducing
shift
polarization
recruited
macrophages
toward
pro-regeneration
(M2)
phenotype.
Our
study
showed
CaNeu-hydrogel‒mediated
resulted
significantly
suppressed
neuroinflammation
apoptosis,
this
phenomenon
involved
PI3K/Akt
signaling
pathway.
findings
indicate
valuable
assist
cell
therapy
SCI,
providing
strategy
central
nervous
system
diseases.
Biophysical Reviews,
Год журнала:
2021,
Номер
13(5), С. 587 - 610
Опубликована: Окт. 1, 2021
Passive
mechanical
tissue
properties
are
major
determinants
of
myocardial
contraction
and
relaxation
and,
thus,
shape
cardiac
function.
Tightly
regulated,
dynamically
adapting
throughout
life,
affecting
a
host
cellular
functions,
passive
mechanics
also
contribute
to
dysfunction.
Development
treatments
early
identification
diseases
requires
better
spatio-temporal
characterisation
their
underlying
mechanisms.
With
this
understanding,
key
regulators
may
be
identified,
providing
pathways
with
potential
control
limit
pathological
development.
Methodologies
models
used
assess
mimic
diverse,
available
data
in
part
mutually
contradictory.
In
review,
we
define
important
concepts
useful
for
characterising
properties,
compare
variety
vitro
vivo
techniques
that
allow
one
mechanics.
We
give
definitions
terms,
summarise
insight
into
stiffness
Frontiers in Cell and Developmental Biology,
Год журнала:
2022,
Номер
10
Опубликована: Ноя. 17, 2022
Macrophages
and
dendritic
cells
are
myeloid
that
play
critical
roles
in
immune
responses.
help
to
maintain
homeostasis
through
tissue
regeneration
the
clearance
of
dead
cells,
but
also
mediate
inflammatory
processes
against
invading
pathogens.
As
most
potent
antigen-presenting
important
connecting
innate
adaptive
responses
Biomedical Materials,
Год журнала:
2022,
Номер
17(2), С. 022007 - 022007
Опубликована: Фев. 15, 2022
Abstract
Implanted
biomaterials
elicit
a
series
of
distinct
immune
and
repair-like
responses
that
are
collectively
known
as
the
foreign
body
reaction
(FBR).
These
include
processes
involving
innate
inflammatory
cells
wound
repair
contribute
to
encapsulation
with
dense
collagenous
largely
avascular
capsule.
Numerous
studies
have
shown
early
phase
is
dominated
by
macrophages
fuse
form
giant
considered
hallmark
FBR.
With
advent
more
precise
cell
characterization
techniques,
specific
macrophage
subsets
been
identified
linked
or
less
favorable
outcomes.
Moreover,
comparing
synthetic-
natural-based
polymer
allowed
identification
subtypes
distinguish
between
fibrotic
regenerative
responses.
More
recently,
associated
adaptive
immunity
participate
in
FBR
synthetic
polymers.
This
suggests
existence
cross-talk
depends
on
nature
implants.
However,
exact
participation
cells,
such
T
B
remains
unclear.
In
fact,
contradictory
suggest
either
independence
dependence
these
cells.
Here,
we
review
evidence
for
involvement
polymers
focus
cellular
molecular
components.
addition,
examine
possibility
induce
antibody
resulting
engagement
Applied Materials Today,
Год журнала:
2022,
Номер
27, С. 101437 - 101437
Опубликована: Март 12, 2022
Biological
cells
and
tissues
are
continuously
subjected
to
mechanical
stress
strain
cues
from
their
surrounding
substrate.
How
these
forces
modulate
cell
tissue
behavior
is
a
major
question
in
mechanobiology.
To
conduct
studies
under
controlled
varying
physiological
scenarios,
new
virtually-assisted
experimental
system
proposed
allowing
for
non-invasive
real-time
control
of
complex
deformation
modes
within
the
substrates.
This
approach
based
on
use
extremely
soft
magneto-active
polymers,
which
mimic
stiffness
biological
materials.
Thus,
enables
untethered
substrates
providing
reversible
changes
controlling
heterogeneous
patterns.
Motivated
deep
magneto-mechanical
characterization
across
scales,
multi-physics
multi-scale
silico
framework
was
developed
guide
stimulation
setup.
The
versatility
viability
have
been
demonstrated
through
its
ability
reproduce
scenarios
simulating
local
patterns
brain
during
head
impact,
capability
transmit
physiologically
relevant
dermal
fibroblasts.
opens
way
understanding
mechanobiological
processes
that
occur
dynamic
states,
e.g.,
traumatic
injury,
pathological
skin
scarring
or
fibrotic
heart
remodeling
myocardial
infarction.
