ACS ES&T Engineering,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 15, 2024
Phototrophs
with
heterotrophic
bacterial
consortium
as
an
electrode
biocatalyst
are
emerging
concept
for
developing
naturally
sustained
biophotovoltaic
systems.
Herein,
Spirulina
subsalsa-based
mixed
community
anodic
catalyst
in
a
microbial
fuel
cell
(MFC)
setup
ferricyanide
catholyte
78
days
light–dark
(16–8
h)
cycle-based
operation
was
investigated.
The
biofilm
developed
inducted
recalcitrant
comprising
Halomonas,
Alcanivorax,
Pelagibacterium,
and
Rhizobiales
the
major
genera.
In
extended
dark
phase
(9
days)
within
cyclic
operation,
sequential
shift
of
metabolism
from
photosynthesis
to
fermentative
states
increased
population
were
observed.
Under
direct
contact
graphite
anode,
initiated
oscillating
open-circuit
potentials
MFC
response
circadian
trend.
delivered
maxima
587
μW
m–2
418
(at
10
kΩ)
under
corresponding
phases,
respectively.
potential
shifted
more
negative
value,
reaching
−415.5
mV
starvation
period.
Analyses
reaction
rates
(extracted
Tafel
plots),
corrosion
potential,
current,
polarization
resistance,
residual
redox
charges
voltammograms)
performed
understand
processes.
Two
peaks
0.6
V
(irreversible,
extracellular)
0.26
(reversible,
cell-surface
attached)
attributed
mediation
this
process.
Additionally,
catholyte-diffused
interacts
biofilm,
getting
trapped
matrix
polymeric
structures,
thus
preventing
sudden
cytotoxic
elimination
cells
promoting
oxidative
charge
accumulation
over
its
surface,
improving
potential.
Rapid
respiratory
oxygen
consumption,
biofilm's
structural
reorganization,
ferricyanide's
chemical
speciation
inside
primary
factors
that
govern
performance
biofuel
during
prolonged
operations.
critical
findings
unveiled
through
study
advance
our
understanding
resilience
phototroph-based
multispecies
catalysts
devices
long-term
Aquatic Microbial Ecology,
Год журнала:
2024,
Номер
90, С. 121 - 139
Опубликована: Июль 29, 2024
A
growing
body
of
literature
has
highlighted
the
importance
phytoplankton-bacterial
associations
to
marine
and
estuarine
ecological
biogeochemical
function,
but
their
population
linkages
remain
sparsely
characterized
within
urban
estuaries.
Since
many
developed
coastlines
are
heavily
impacted
by
anthropogenic
nutrient
inputs,
elucidating
dynamics
provides
insight
into
cycling,
productivity,
can
help
inform
water
quality
management.
This
study
compared
surface
(0.5
m
depth)
physical
quality,
cell
abundances
major
phytoplankton
taxa
bacteria,
as
well
concentrations
chlorophyll
a
(chl
)
dissolved
organic
matter
(DOM)
in
nitrogen
(N)-enriched
Western
Long
Island
Sound
(WLIS),
USA,
between
mid-channel
shore
sites
(in
2020
2021).
Shore
bacterial
DOM
(primarily
N
carbon
[DOC]),
were
significantly
higher
than
mid-channel,
especially
during
summer,
indicative
terrestrial
loading
influencing
microbial
assemblages
C
cycling.
Abundances
key
better
indicators
chl
,
positively
correlated
with
those
dinoflagellates,
most
common
genera
Prorocentrum
(mid-channel,
shore)
Heterocapsa
(shore
only),
not
diatoms.
However,
pennate
diatom
negatively
DOC
mid-channel.
Results
highlight
impact
inputs
on
WLIS
assemblage
dynamics,
presumably
favoring
bacteria
dinoflagellate
coupling,
shed
new
how
communities
respond
loadings
Bacterial
membrane
vesicles
(BMVs)
are
crucial
biological
vehicles
for
facilitating
interspecies
and
interkingdom
interactions.
However,
the
extent
mechanisms
of
BMV
involvement
in
bacterial-algal
communication
remain
elusive.
This
study
provides
evidence
BMVs
delivering
cargos
to
targeted
microalgae.
Membrane
(MVs)
from
Chitinimonas
prasina
LY03
demonstrated
an
algicidal
profile
similar
strain
LY03.
Further
investigation
revealed
Tambjamine
LY2,
effective
compound,
selectively
packaged
into
LY03-MVs.
Microscopic
imaging
efficient
delivery
LY2
microalgae
Heterosigma
akashiwo
Thalassiosira
pseudonana
through
fusion.
In
addition,
versatile
cargo
capabilities
algae,
including
transfer
MV-carried
nucleic
acids
algal
cells
revival
growth
iron-depleted
by
MVs.
Collectively,
our
findings
reveal
a
previously
unknown
mechanism
which
bacteria
store
hydrophobic
compounds
MVs
trigger
target
death
highlight
potency
understanding
engineering
bacterial-algae
cross-talk.
Scientific Reports,
Год журнала:
2024,
Номер
14(1)
Опубликована: Окт. 25, 2024
Metabolite
exchange
mediates
crucial
interactions
in
microbial
communities,
significantly
impacting
global
carbon
and
nitrogen
cycling.
Understanding
these
chemically-mediated
is
essential
for
elucidating
natural
community
functions
developing
engineered
synthetic
communities.
This
study
investigated
membrane-separated
bioreactors
(mBRs)
as
a
novel
tool
to
identify
transient
metabolites
their
producers/consumers
mixed
We
compared
three
co-culture
methods
(direct
mixed,
2-chamber
mBR,
3-chamber
mBR)
grow
binary
of
the
cyanobacterium
Synechococcus
elongatus
PCC
7942
fungus
Rhodotorula
toruloides
NBRC
0880,
well
axenic
S.
elongatus.
Despite
not
being
lichen
constituents,
organisms
exhibited
resembling
those
cyanolichens.
fixed
CO
ACS ES&T Engineering,
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 15, 2024
Phototrophs
with
heterotrophic
bacterial
consortium
as
an
electrode
biocatalyst
are
emerging
concept
for
developing
naturally
sustained
biophotovoltaic
systems.
Herein,
Spirulina
subsalsa-based
mixed
community
anodic
catalyst
in
a
microbial
fuel
cell
(MFC)
setup
ferricyanide
catholyte
78
days
light–dark
(16–8
h)
cycle-based
operation
was
investigated.
The
biofilm
developed
inducted
recalcitrant
comprising
Halomonas,
Alcanivorax,
Pelagibacterium,
and
Rhizobiales
the
major
genera.
In
extended
dark
phase
(9
days)
within
cyclic
operation,
sequential
shift
of
metabolism
from
photosynthesis
to
fermentative
states
increased
population
were
observed.
Under
direct
contact
graphite
anode,
initiated
oscillating
open-circuit
potentials
MFC
response
circadian
trend.
delivered
maxima
587
μW
m–2
418
(at
10
kΩ)
under
corresponding
phases,
respectively.
potential
shifted
more
negative
value,
reaching
−415.5
mV
starvation
period.
Analyses
reaction
rates
(extracted
Tafel
plots),
corrosion
potential,
current,
polarization
resistance,
residual
redox
charges
voltammograms)
performed
understand
processes.
Two
peaks
0.6
V
(irreversible,
extracellular)
0.26
(reversible,
cell-surface
attached)
attributed
mediation
this
process.
Additionally,
catholyte-diffused
interacts
biofilm,
getting
trapped
matrix
polymeric
structures,
thus
preventing
sudden
cytotoxic
elimination
cells
promoting
oxidative
charge
accumulation
over
its
surface,
improving
potential.
Rapid
respiratory
oxygen
consumption,
biofilm's
structural
reorganization,
ferricyanide's
chemical
speciation
inside
primary
factors
that
govern
performance
biofuel
during
prolonged
operations.
critical
findings
unveiled
through
study
advance
our
understanding
resilience
phototroph-based
multispecies
catalysts
devices
long-term
