Characterization of the male-specific region containing the candidate sex-determining gene in Amur catfish (Silurus asotus) using third-generation- and pool-sequencing data

International Journal of Biological Macromolecules, Год журнала: 2023, Номер 248, С. 125908 - 125908

Опубликована: Июль 22, 2023

Язык: Английский

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Dose-dependent role of AMH and AMHR2 signaling in male differentiation and regulation of sex determination in Spotted knifejaw (Oplegnathus punctatus) with X1X1X2X2/X1X2Y chromosome system

Cell Communication and Signaling, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 1, 2025

Sex determination mechanisms vary significantly across different chromosomal systems and evolutionary contexts. Nonetheless, the regulatory framework governing multi-sex chromosome system (X1X1X2X2/X1X2Y) remains enigmatic. Through an examination of sex-related genes (dmrt1, hsd11b2, amh, sox9a, sox9b, foxl2, cyp19a), hormonal influences (E2, 11-KT), histological analyses gonadal development, we demonstrate that critical period for sexual differentiation occurs between 35 to 60 days post-hatching (dph). Our multi-omics analysis identified amhr2 as a candidate sex-determining gene, revealing males possess three distinct transcripts (amhr2ay, amhr2by, amhr2cy), whereas females express only one (amhr2a). In situ hybridization assays demonstrated is predominantly localized primary spermatocyte Sertoli cells male testes. Notably, specific mRNA expression enriched in amhr2cy, whose extracellular domain exhibits highest binding affinity Amh protein, with differences manifesting early 5 dph. The outcomes interference (RNAi) experiments indicate knockdown leads reduction male-related gene sox9b), androgen synthesis (hsd11b2, cyp11a), female-related (wnt4, cyp19a, cyp19b). Conversely, overexpression yielded contrasting results. research supports role pivotal gene. Furthermore, dosage effect amhr2, reflected transcript abundance, levels, efficacy, serves fundamental mechanism driving processes Spotted knifejaw.

Язык: Английский

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The origin, evolution, and translocation of sex chromosomes in Silurus catfish mediated by transposons

BMC Biology, Год журнала: 2025, Номер 23(1)

Опубликована: Фев. 21, 2025

Sex chromosome (SC) evolution is a longstanding topic of focus in evolutionary biology. Teleosts often exhibit rapid turnover SCs and sex-determining (SD) genes, alongside diverse range SC differentiation mechanisms. On the basis new chromosome-scale assemblies three Silurus species (S. microdorsalis, S. glanis, lanzhouensis) two outgroup (Pterocryptis cochinchinensis Kryptopterus bicirrhis), along with our previous meridionalis asotus, we traced genus (Siluriformes), following fate known SD gene amhr2y. Phylogenetic analysis showed that amhr2y occurred at least before divergence Pterocryptis, Kryptopterus, lost P. K. bicirrhis. Chr24 has become ancestor five due to duplication-and-translocation amhr2 mediated by LTR transposon. Then, proto Y was formed maintained shared 60 kb male-specific region (MSY) transposable elements (TEs) expansion gathering. Due continuous TEs accumulation, genes other than MSYs have degenerated or been lost, while non-recombinant regions continue expend, forming different sizes (from 320 550 kb). Two events, one homologous left arm right Chr24) nonhomologous Chr5), occurring among were possibly hAT Helitron transposons. Our results on dynamic trajectory amhr2y, MSYs, catfish indicated variability diversity fish confirmed frequent an important way maintain homology low SCs.

Язык: Английский

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Molecular parallelism in the evolution of a master sex‐determining role for the anti‐Mullerian hormone receptor 2 gene (amhr2) in Midas cichlids

Molecular Ecology, Год журнала: 2022, Номер 32(6), С. 1398 - 1410

Опубликована: Апрель 11, 2022

The evolution of sex chromosomes and their differentiation from autosomes is a major event during genome that happened many times in several lineages. repeated lability sex-determination mechanisms fishes makes this well-suited system to test for general patterns evolution. According current theory, triggered by the suppression recombination following new master sex-determining gene. However, molecular establish are known few examples, owing intrinsic difficulties assembling regions (SDRs). development forward-genetics long-read sequencing have generated wealth data questioning central aspects theory. Here, we demonstrate Midas cichlids determined an XY system, identify assemble SDR combining forward-genetics, optical mapping. We show how long-reads aid detection artefacts genotype-phenotype mapping arise incomplete assemblies. male-specific region restricted 100-kb segment on chromosome 4 harbours transposable elements Y-specific duplicate anti-Mullerian receptor 2 gene, which has evolved functions repeatedly. Our suggest amhr2Y originated interchromosomal translocation 20 pre-dating split Flier cichlids. In latter, it pseudogenized translocated another chromosome. Duplication genes common route establishing determiners, highlighting role parallelism determination.

Язык: Английский

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Sex chromosome cycle as a mechanism of stable sex determination

The Journal of Biochemistry, Год журнала: 2024, Номер 176(2), С. 81 - 95

Опубликована: Июль 10, 2024

Abstract Recent advances in DNA sequencing technology have enabled the precise decoding of genomes non-model organisms, providing a basis for unraveling patterns and mechanisms sex chromosome evolution. Studies different species yielded conflicting results regarding traditional theory that chromosomes evolve from autosomes via accumulation deleterious mutations degeneration Y (or W) chromosome. The concept ‘sex cycle,’ emerging this context, posits at any stage cycle (i.e., differentiation, degeneration, or loss), turnover can occur while maintaining stable determination. Thus, understanding drive both persistence each is crucial. In review, we integrate recent findings on underlying maintenance turnover, with special focus several organisms having unique chromosomes. Our review suggests diversity determination underappreciated emphasizes need more research cycle.

Язык: Английский

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Chromosome-level genome assembly and annotation of largemouth bronze gudgeon (Coreius guichenoti)

Scientific Data, Год журнала: 2025, Номер 12(1)

Опубликована: Янв. 15, 2025

Coreius guichenoti, mainly distributed in upstream regions of the Yangtze River China, is currently on brink extinction and listed as national secondary protected animal. In this study, we aimed to obtain chromosome-level genome C. guichenoti using PacBio Hi-C techniques. According sequencing, was successfully assembled 1100.1 Mb size, with a Contig N50 size 25.0 Mb, containing 731.0 repeats. sequencing data utilized for chromosome assembly 25 sequences were ultimately yielded, total length 1076.8 Mb. Moreover, 22,506 protein-coding genes predicted average intron 2293 bp. Evolutionary analysis divergence time prediction revealed that closely related heterodon they phylogenetically diverged from common ancestor ~20.7 million years ago (Mya), following separation Cyprinidae at 28.3 Mya. future, utilization comparative genomics research important elucidating molecular mechanisms Ichthyophthirius disease ensuring conservation biological resources.

Язык: Английский

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Single-cell sequencing provides clues about the developmental genetic basis of evolutionary adaptations in syngnathid fishes

eLife, Год журнала: 2025, Номер 13

Опубликована: Фев. 3, 2025

Seahorses, pipefishes, and seadragons are fishes from the family Syngnathidae that have evolved extraordinary traits including male pregnancy, elongated snouts, loss of teeth, dermal bony armor. The developmental genetic cellular changes led to evolution these largely unknown. Recent syngnathid genome assemblies revealed suggestive gene content differences provided opportunity for detailed analyses. We created a single-cell RNA sequencing atlas Gulf pipefish embryos understand basis four traits: derived head shape, toothlessness, armor, pregnancy. completed marker analyses, built networks, examined spatial expression select genes. identified osteochondrogenic mesenchymal cells in elongating face express regulatory genes bmp4, sfrp1a , prdm16 . found no evidence tooth primordia cells, we observed re-deployment osteoblast networks developing Finally, epidermal expressed nutrient processing environmental sensing genes, potentially relevant brooding environment. evolutionary innovations composed recognizable cell types, suggesting features originate within existing networks. Future work addressing across multiple stages species is essential understanding how novelties fish evolved.

Язык: Английский

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The Cause–Effect Model of Master Sex Determination Gene Acquisition and the Evolution of Sex Chromosomes

International Journal of Molecular Sciences, Год журнала: 2025, Номер 26(7), С. 3282 - 3282

Опубликована: Апрель 1, 2025

The canonical model of vertebrate sex chromosome evolution predicts a one-way trend toward degradation. However, most chromosomes in lower vertebrates are homomorphic. Recent progress studies determination has resulted the discovery more than 30 master (MSD) genes, which from teleost fish. An analysis MSD gene acquisition, recombination suppression, and chromosome-specific sequences revealed correlations modes acquisition chromosomes. Sex remain homomorphic with genes acquired by simple mutations, duplications, allelic variations, or neofunctionalization; contrast, they become heteromorphic chromosomal inversion, fusion, fission. There is no suppression carrying gained through mutations. In there extensive inversion. limited transposition translocation. We propose cause-effect that as consequence explains various vertebrates. A key factor determining whether non-homologous regions created during genes. Chromosome inversion creates inversely homologous but directly sequences, lead to retain potential. Over time, recurrent inverted leads formation strata may cause degradation Depending on nature deletions regions, evolve dosage compensation, selective retention haplo-insufficient be used an alternative strategy.

Язык: Английский

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Phylogenetic diversifications linked to the morphological traits and global phylogeographic pattern of closed pouch fishes in Syngnathidae

The Innovation Geoscience, Год журнала: 2025, Номер unknown, С. 100137 - 100137

Опубликована: Янв. 1, 2025

<p>Syngnathids, distinguished by their unique morphology and male pregnancy, serve as flagship species in global dispersal colonization studies. There is high morphological variation among Syngnathidae, but the relationship between phylogeographic patterns still requires further investigation. Here, we analyzed mitochondrial genomes of 48 Syngnathidae to clarify phylogenetic relationships divergence times. The traits has been revealed based on reconstructing ancestral status seahorses, pipehorses, pipefishes, seadragons. We show that <i>Hippocampus</i> <i>Syngnathus</i> with enclosed brood pouches are likely associated higher biodiversity broader distribution. Moreover, comparing worldwide biogeographic pipefishes our results indicate they have distinct origin centres colonized globally, which might be influenced ocean currents historical climatic shifts during Miocene.</p>

Язык: Английский

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Improving conservation outcomes for seadragons: data, knowledge gaps, and future directions

Reviews in Fish Biology and Fisheries, Год журнала: 2025, Номер unknown

Опубликована: Май 21, 2025

Язык: Английский

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