POSA for fast, amplified and multiplexed protein imaging

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 7, 2025

Fluorescent π-conjugated polymers (FCPs) are known for their superior brightness but still unavailable highly multiplexed molecular imaging in single cells as they hydrophobic and lack targeting capability toward biomolecules. Herein, we develop a polymer-based amplification (POSA) method to achieve signal amplification. Optical by virtue of the high FCPs makes POSA simple quick technique that can spatially resolve distribution proteins cells, with 28- 126-fold effect. By this method, demonstrate be used strengthen images subcellular biomolecules showcase phenomenon optical at cellular level. Additionally, its sensitivity, ease use, features, proves valuable tool advanced biological research. brightness, but, thus far, use has been negligible. Here, authors propose POSA,

Язык: Английский

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DNA Scaffold-Enhanced Hybrid Chain Reaction for Molecular Diagnosis of miRNAs

Journal of Analysis and Testing, Год журнала: 2025, Номер unknown

Опубликована: Март 10, 2025

Язык: Английский

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Unravelling tumour spatiotemporal heterogeneity using spatial multimodal data

Clinical and Translational Medicine, Год журнала: 2025, Номер 15(5)

Опубликована: Май 1, 2025

Язык: Английский

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Extended Linear Confined Zipper Cascaded Reaction for Highly Efficient Intracellular Imaging and Assisting Diagnosis of Thyroid Cancer

Small, Год журнала: 2025, Номер unknown

Опубликована: Май 23, 2025

Abstract Highly sensitive detection and in situ tracing analysis of small‐molecule biomarkers are particularly indispensable to deciphering the pathogenesis pathological process. Despite DNA assembly‐based barcoding amplification strategies across breadth molecular analysis, an easy‐to‐design, nonenzymatic, highly efficient, background leakage‐avoided, specific, system is required yet still its infancy. Spatial confinement nano‐assembly can increase reaction efficiency a localized isothermal autonomous manner. Here this work, assembly that originally relies on random collisions between freely diffusing probes constructed two extended linear confined probes, by which novel model named as zipper hybridization chain (ZHCR ) proposed. ZHCR significantly improve probe enable stable within live cells, providing precise target information. employed analyze thyroid cancer‐specific miRNAs, achieving serum content detection. By integrating machine learning algorithms, demonstrates significant potential cancer auxiliary diagnosis, establishing versatile platform enables both homogeneous low‐abundance nucleic acid fragments.

Язык: Английский

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Programming gel automata shapes using DNA instructions

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Сен. 5, 2024

Язык: Английский

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