A Mesenchymal stem cell Aging Framework, from Mechanisms to Strategies
Stem Cell Reviews and Reports,
Год журнала:
2024,
Номер
20(6), С. 1420 - 1440
Опубликована: Май 10, 2024
Язык: Английский
Cyclic Stretch Promotes Cellular Reprogramming Process through Cytoskeletal‐Nuclear Mechano‐Coupling and Epigenetic Modification
Advanced Science,
Год журнала:
2023,
Номер
10(32)
Опубликована: Сен. 19, 2023
Abstract
Advancing
the
technologies
for
cellular
reprogramming
with
high
efficiency
has
significant
impact
on
regenerative
therapy,
disease
modeling,
and
drug
discovery.
Biophysical
cues
can
tune
cell
fate,
yet
precise
role
of
external
physical
forces
during
remains
elusive.
Here
authors
show
that
temporal
cyclic‐stretching
fibroblasts
significantly
enhances
induced
pluripotent
stem
(iPSC)
production.
Generated
iPSCs
are
proven
to
express
pluripotency
markers
exhibit
in
vivo
functionality.
Bulk
RNA‐sequencing
reveales
biological
characteristics
required
acquisition,
including
increased
division
mesenchymal‐epithelial
transition.
Of
note,
activates
key
mechanosensitive
molecules
(integrins,
perinuclear
actins,
nesprin‐2,
YAP),
across
cytoskeletal‐to‐nuclear
space.
Furthermore,
stretch‐mediated
cytoskeletal‐nuclear
mechano‐coupling
leads
altered
epigenetic
modifications,
mainly
downregulation
H3K9
methylation,
its
global
gene
occupancy
change,
as
revealed
by
genome‐wide
ChIP‐sequencing
pharmacological
inhibition
tests.
Single
further
identifies
subcluster
mechano‐responsive
modifier
stretched
cells.
Collectively,
iPSC
through
mechanotransduction
process
changes
accompanied
genes.
This
study
highlights
strong
link
between
subsequent
expression
related
genes
reprogramming,
holding
substantial
implications
field
biology,
tissue
engineering,
medicine.
Язык: Английский
An engineered Sox17 induces somatic to neural stem cell fate transitions independently from pluripotency reprogramming
Science Advances,
Год журнала:
2023,
Номер
9(34)
Опубликована: Авг. 23, 2023
Advanced
strategies
to
interconvert
cell
types
provide
promising
avenues
model
cellular
pathologies
and
develop
therapies
for
neurological
disorders.
Yet,
methods
directly
transdifferentiate
somatic
cells
into
multipotent
induced
neural
stem
(iNSCs)
are
slow
inefficient,
it
is
unclear
whether
pass
through
a
pluripotent
state
with
full
epigenetic
reset.
We
report
iNSC
reprogramming
from
embryonic
aged
mouse
fibroblasts
as
well
human
blood
using
an
engineered
Sox17
(eSox17FNV).
eSox17FNV
efficiently
drives
while
Sox2
or
fail.
acquires
the
capacity
bind
different
protein
partners
on
regulatory
DNA
scan
genome
more
has
potent
transactivation
domain
than
Sox2.
Lineage
tracing
time-resolved
transcriptomics
show
that
emerging
iNSCs
do
not
transit
state.
Our
work
distinguishes
lineage
pluripotency
potential
generate
authentic
models
aging-associated
neurodegenerative
diseases.
Язык: Английский
Metabolic control of induced pluripotency
Frontiers in Cell and Developmental Biology,
Год журнала:
2024,
Номер
11
Опубликована: Янв. 11, 2024
Pluripotent
stem
cells
of
the
mammalian
epiblast
and
their
cultured
counterparts—embryonic
(ESCs)
(EpiSCs)—have
capacity
to
differentiate
in
all
cell
types
adult
organisms.
An
artificial
process
reactivation
pluripotency
program
terminally
differentiated
was
established
2006,
which
allowed
for
generation
induced
pluripotent
(iPSCs).
This
iPSC
technology
has
become
an
invaluable
tool
investigating
molecular
mechanisms
human
diseases
therapeutic
drug
development,
it
also
holds
tremendous
promise
applications
regenerative
medicine.
Since
reprogramming
a
state
discovered,
many
questions
about
involved
this
have
been
clarified.
Studies
conducted
over
past
2
decades
that
metabolic
pathways
retrograde
mitochondrial
signals
are
regulation
various
aspects
biology,
including
differentiation,
acquisition,
maintenance.
During
process,
undergo
major
transformations,
progressing
through
three
distinct
stages
regulated
by
different
signaling
pathways,
transcription
factor
networks,
inputs
from
pathways.
Among
main
features
representing
switch
dominance
oxidative
phosphorylation
aerobic
glycolysis
anabolic
processes,
critical
stage-specific
control
path
toward
state.
In
review,
we
discuss
achievements
current
understanding
processes
controlled
vice
versa,
during
process.
Язык: Английский
Zinc oxide nanoparticles damage the prefrontal lobe in mouse: Behavioral impacts and key mechanisms
Toxicology Letters,
Год журнала:
2024,
Номер
397, С. 129 - 140
Опубликована: Май 16, 2024
Язык: Английский
Desmosomes in Cell Fate Determination: From Cardiogenesis to Cardiomyopathy
Cells,
Год журнала:
2023,
Номер
12(17), С. 2122 - 2122
Опубликована: Авг. 22, 2023
Desmosomes
play
a
vital
role
in
providing
structural
integrity
to
tissues
that
experience
significant
mechanical
tension,
including
the
heart.
Deficiencies
desmosomal
proteins
lead
development
of
arrhythmogenic
cardiomyopathy
(AC).
The
limited
availability
preventative
measures
clinical
settings
underscores
pressing
need
gain
comprehensive
understanding
not
only
cardiomyocytes
but
also
non-myocyte
residents
heart,
as
they
actively
contribute
progression
cardiomyopathy.
This
review
focuses
specifically
on
impact
desmosome
deficiency
epi-
and
endocardial
cells.
We
highlight
intricate
cross-talk
between
mutations
signaling
pathways
involved
regulation
epicardial
cell
fate
transition.
further
emphasize
consequences
differ
embryonic
adult
heart
leading
enhanced
erythropoiesis
during
fibrogenesis
mature
suggest
triggering
epi-/endocardial
cells
fibroblasts
are
different
“states”
involve
same
pathological
outcomes.
Understanding
details
responses
must
be
considered
when
developing
interventions
therapeutic
strategies.
Язык: Английский
Graft of cardiac progenitors in a pig model of right ventricular failure triggers myocardial epimorphosis, regeneration and protection of function
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2022,
Номер
unknown
Опубликована: Июль 4, 2022
The
failure
of
diseased
adult
heart
to
regenerate
is
a
major
burden
our
societies.
Besides
patients
with
ischemia
and
left
ventricular
dysfunction,
progress
in
pediatric
surgery
repair
cardiac
malformations
has
led
growing
population
now
congenital
diseases
right
failure.
In
the
absence
any
efficient
pharmacological
therapy
for
these
patients,
cell
turned
out
be
only
option
RV
myocardium.
this
study,
we
demonstrate
that
pig
failure,
model
repaired
tetralogy
Fallot,
ability
regenerative
epimorphosis.
Human
embryonic
stem
cell-derived
Nkx2.5+
progenitor
cells
were
seeded
collagen
based
patch
cover
whole
failing
RV.
We
report
migrate
within
myocardium
while
reversing
interstitial
fibrosis.
They
then
engraft
fully
differentiate
into
fetal-like
human
myocytes
graft
triggers
reprogramming
surrounding
Oct4
+
/Nanog
-
blastemal-like
cells.
reprogrammed
re-differentiate
proliferate
around
myocytes.
Altogether,
findings
reveal
mammalian
hearts
have
undergo
epimorphosis,
process
endogenous
regeneration
leads
recovery
their
contractile
function.
Язык: Английский