aBIOTECH,
Год журнала:
2023,
Номер
4(3), С. 202 - 212
Опубликована: Июнь 28, 2023
Abstract
Induced
mutations
are
important
for
genetic
research
and
breeding.
Mutations
induced
by
physical
or
chemical
mutagenesis
usually
heterozygous
during
the
early
generations.
However,
must
be
fixed
prior
to
phenotyping
field
trials,
which
requires
additional
rounds
of
self-pollination.
Microspore
culture
is
an
effective
method
produce
double-haploid
(DH)
plants
that
homozygotes.
In
this
study,
we
conducted
ethyl
methanesulfonate
(EMS)-induced
microspore
cultures
barley
(
Hordeum
vulgare
)
cultivar
‘Hua30’
landrace
‘HTX’.
The
EMS
concentrations
were
negatively
correlated
with
efficiency
callus
induction
frequency
mutant
plant
regeneration.
two
genotypes
showed
different
regeneration
efficiencies.
phenotypic
variation
regenerated
M
1
presence
genome-wide
nucleotide
mutations,
revealed
whole-genome
sequencing,
highlight
utility
EMS-induced
isolated
developing
DH
mutants.
Genome-wide
analysis
mutation
in
a
considerable
proportion
resulted
from
(somaclonal
variation)
rather
than
mutagenesis.
addition
producing
population
1972
homozygous
lines
available
future
study
lays
foundation
optimizing
richness
(mainly
fine-tuning
mutagen
dosage).
Scotch
Whisky,
a
product
of
high
importance
to
Scotland,
has
gained
global
approval
for
its
distinctive
qualities
derived
from
the
traditional
production
process,
which
is
defined
in
law.
However,
ongoing
research
continuously
enhances
Whisky
and
fostering
diversification
flavour
profiles.
To
be
classified
as
final
spirit
needs
retain
aroma
taste
'Scotch'.
While
each
step
contributes
significantly
whisky
flavour-from
malt
preparation
mashing
fermentation,
distillation,
maturation-the
impact
yeast
during
fermentation
crucially
important.
Not
only
does
convert
sugar
alcohol,
it
also
produces
important
volatile
compounds,
e.g.
esters
higher
alcohols,
that
contribute
profile
whisky.
The
chosen
fermentations
can
influence
flavour,
so
strain
employed
importance.
This
review
explores
role
on
diversification.
Furthermore,
an
extensive
examination
nonconventional
yeasts
brewing
winemaking
undertaken
assess
their
potential
suitability
adoption
strains,
followed
by
methods
evaluating
new
strains.
New Biotechnology,
Год журнала:
2024,
Номер
82, С. 92 - 106
Опубликована: Май 22, 2024
Species
of
Saccharomyces
genus
have
played
an
irreplaceable
role
in
alcoholic
beverage
and
baking
industry
for
centuries.
S.
cerevisiae
has
also
become
organism
choice
industrial
production
alcohol
other
valuable
chemicals
a
model
shaping
the
rise
modern
genetics
genomics
past
few
decades.
Today´s
brewing
faces
challenges
decreasing
consumption
traditional
beer
styles
increasing
consumer
demand
new
styles,
flavors
aromas.
The
number
currently
used
brewer's
strains
their
genetic
diversity
is
yet
limited
implementation
more
phenotypic
variation
seen
as
solution
to
cope
with
market
challenges.
This
requires
modification
current
or
introduction
novel
from
settings,
e.g.
wild
habitats
into
industry.
Due
legal
regulation
many
countries
negative
customer
perception
GMO
organisms,
food
beverages
non-GMO
whose
development
can
be
difficult
time-consuming.
Here,
we
apply
FIND-IT
(Fast
Identification
Nucleotide
variants
by
DigITal
PCR),
ultrafast
genome-mining
method,
isolation
yeast
varying
flavor
profiles.
method
uses
combination
random
mutagenesis,
droplet
digital
PCR
probes
that
target
specific
desired
mutation
sub-isolation
mutant
clone.
Such
approach
allows
targeted
identification
eliminated
certain
off-flavors
and/or
changes
strain
metabolism.
We
demonstrate
technology
useful
loss-of
function
gain
mutations
unrelated
differing
ploidy.
Where
no
selection
exists,
this
serves
together
standard
breeding
techniques
tool
facilitating
(brewer's)
leading
diversification
product
portfolio.
Current Opinion in Biotechnology,
Год журнала:
2024,
Номер
88, С. 103147 - 103147
Опубликована: Июнь 3, 2024
Plant
natural
products
have
been
an
important
source
of
medicinal
molecules
since
ancient
times.
To
gain
access
to
the
whole
diversity
these
for
pharmaceutical
applications,
it
is
understand
their
biosynthetic
origins.
Whilst
co-expression
a
reliable
tool
identifying
gene
candidates,
variety
complementary
methods
can
aid
in
screening
or
refining
candidate
selection.
Here,
we
review
recently
employed
plant
pathway
discovery
approaches,
and
highlight
future
directions
field.
aBIOTECH,
Год журнала:
2023,
Номер
4(3), С. 202 - 212
Опубликована: Июнь 28, 2023
Abstract
Induced
mutations
are
important
for
genetic
research
and
breeding.
Mutations
induced
by
physical
or
chemical
mutagenesis
usually
heterozygous
during
the
early
generations.
However,
must
be
fixed
prior
to
phenotyping
field
trials,
which
requires
additional
rounds
of
self-pollination.
Microspore
culture
is
an
effective
method
produce
double-haploid
(DH)
plants
that
homozygotes.
In
this
study,
we
conducted
ethyl
methanesulfonate
(EMS)-induced
microspore
cultures
barley
(
Hordeum
vulgare
)
cultivar
‘Hua30’
landrace
‘HTX’.
The
EMS
concentrations
were
negatively
correlated
with
efficiency
callus
induction
frequency
mutant
plant
regeneration.
two
genotypes
showed
different
regeneration
efficiencies.
phenotypic
variation
regenerated
M
1
presence
genome-wide
nucleotide
mutations,
revealed
whole-genome
sequencing,
highlight
utility
EMS-induced
isolated
developing
DH
mutants.
Genome-wide
analysis
mutation
in
a
considerable
proportion
resulted
from
(somaclonal
variation)
rather
than
mutagenesis.
addition
producing
population
1972
homozygous
lines
available
future
study
lays
foundation
optimizing
richness
(mainly
fine-tuning
mutagen
dosage).
