Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment DOI Creative Commons

Linli Huang,

Guangqi Gao, Congcong Jiang

и другие.

aBIOTECH, Год журнала: 2023, Номер 4(3), С. 202 - 212

Опубликована: Июнь 28, 2023

Abstract Induced mutations are important for genetic research and breeding. Mutations induced by physical or chemical mutagenesis usually heterozygous during the early generations. However, must be fixed prior to phenotyping field trials, which requires additional rounds of self-pollination. Microspore culture is an effective method produce double-haploid (DH) plants that homozygotes. In this study, we conducted ethyl methanesulfonate (EMS)-induced microspore cultures barley ( Hordeum vulgare ) cultivar ‘Hua30’ landrace ‘HTX’. The EMS concentrations were negatively correlated with efficiency callus induction frequency mutant plant regeneration. two genotypes showed different regeneration efficiencies. phenotypic variation regenerated M 1 presence genome-wide nucleotide mutations, revealed whole-genome sequencing, highlight utility EMS-induced isolated developing DH mutants. Genome-wide analysis mutation in a considerable proportion resulted from (somaclonal variation) rather than mutagenesis. addition producing population 1972 homozygous lines available future study lays foundation optimizing richness (mainly fine-tuning mutagen dosage).

Язык: Английский

The potential for Scotch Malt Whisky flavour diversification by yeast DOI Creative Commons
Martina Daute, Frances Jack, Graeme M. Walker

и другие.

FEMS Yeast Research, Год журнала: 2024, Номер 24

Опубликована: Янв. 1, 2024

Scotch Whisky, a product of high importance to Scotland, has gained global approval for its distinctive qualities derived from the traditional production process, which is defined in law. However, ongoing research continuously enhances Whisky and fostering diversification flavour profiles. To be classified as final spirit needs retain aroma taste 'Scotch'. While each step contributes significantly whisky flavour-from malt preparation mashing fermentation, distillation, maturation-the impact yeast during fermentation crucially important. Not only does convert sugar alcohol, it also produces important volatile compounds, e.g. esters higher alcohols, that contribute profile whisky. The chosen fermentations can influence flavour, so strain employed importance. This review explores role on diversification. Furthermore, an extensive examination nonconventional yeasts brewing winemaking undertaken assess their potential suitability adoption strains, followed by methods evaluating new strains.

Язык: Английский

Процитировано

2

Modifying flavor profiles of Saccharomyces spp. for industrial brewing using FIND-IT, a non-GMO approach for metabolic engineering of yeast DOI Creative Commons
Vratislav Šťovíček,

Klaus B. Lengeler,

Toni Wendt

и другие.

New Biotechnology, Год журнала: 2024, Номер 82, С. 92 - 106

Опубликована: Май 22, 2024

Species of Saccharomyces genus have played an irreplaceable role in alcoholic beverage and baking industry for centuries. S. cerevisiae has also become organism choice industrial production alcohol other valuable chemicals a model shaping the rise modern genetics genomics past few decades. Today´s brewing faces challenges decreasing consumption traditional beer styles increasing consumer demand new styles, flavors aromas. The number currently used brewer's strains their genetic diversity is yet limited implementation more phenotypic variation seen as solution to cope with market challenges. This requires modification current or introduction novel from settings, e.g. wild habitats into industry. Due legal regulation many countries negative customer perception GMO organisms, food beverages non-GMO whose development can be difficult time-consuming. Here, we apply FIND-IT (Fast Identification Nucleotide variants by DigITal PCR), ultrafast genome-mining method, isolation yeast varying flavor profiles. method uses combination random mutagenesis, droplet digital PCR probes that target specific desired mutation sub-isolation mutant clone. Such approach allows targeted identification eliminated certain off-flavors and/or changes strain metabolism. We demonstrate technology useful loss-of function gain mutations unrelated differing ploidy. Where no selection exists, this serves together standard breeding techniques tool facilitating (brewer's) leading diversification product portfolio.

Язык: Английский

Процитировано

2

Beyond co-expression: pathway discovery for plant pharmaceuticals DOI Creative Commons
Sandesh H Swamidatta, Benjamin R. Lichman

Current Opinion in Biotechnology, Год журнала: 2024, Номер 88, С. 103147 - 103147

Опубликована: Июнь 3, 2024

Plant natural products have been an important source of medicinal molecules since ancient times. To gain access to the whole diversity these for pharmaceutical applications, it is understand their biosynthetic origins. Whilst co-expression a reliable tool identifying gene candidates, variety complementary methods can aid in screening or refining candidate selection. Here, we review recently employed plant pathway discovery approaches, and highlight future directions field.

Язык: Английский

Процитировано

2

Sharp solutions to cleave plant fibers DOI

Talia Jacobson,

M.F. Edwards, Cătălin Voiniciuc

и другие.

Current Opinion in Biotechnology, Год журнала: 2024, Номер 90, С. 103219 - 103219

Опубликована: Окт. 24, 2024

Язык: Английский

Процитировано

2

Generating homozygous mutant populations of barley microspores by ethyl methanesulfonate treatment DOI Creative Commons

Linli Huang,

Guangqi Gao, Congcong Jiang

и другие.

aBIOTECH, Год журнала: 2023, Номер 4(3), С. 202 - 212

Опубликована: Июнь 28, 2023

Abstract Induced mutations are important for genetic research and breeding. Mutations induced by physical or chemical mutagenesis usually heterozygous during the early generations. However, must be fixed prior to phenotyping field trials, which requires additional rounds of self-pollination. Microspore culture is an effective method produce double-haploid (DH) plants that homozygotes. In this study, we conducted ethyl methanesulfonate (EMS)-induced microspore cultures barley ( Hordeum vulgare ) cultivar ‘Hua30’ landrace ‘HTX’. The EMS concentrations were negatively correlated with efficiency callus induction frequency mutant plant regeneration. two genotypes showed different regeneration efficiencies. phenotypic variation regenerated M 1 presence genome-wide nucleotide mutations, revealed whole-genome sequencing, highlight utility EMS-induced isolated developing DH mutants. Genome-wide analysis mutation in a considerable proportion resulted from (somaclonal variation) rather than mutagenesis. addition producing population 1972 homozygous lines available future study lays foundation optimizing richness (mainly fine-tuning mutagen dosage).

Язык: Английский

Процитировано

4