A genome-wide screen identifies silencers with distinct chromatin properties and mechanisms of repression DOI Creative Commons
Lorena Hofbauer, Lisa‐Marie Pleyer,

Franziska Reiter

и другие.

Molecular Cell, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Differential gene transcription enables development and homeostasis in all animals is regulated by two major classes of distal cis-regulatory DNA elements (CREs): enhancers silencers. Although have been thoroughly characterized, the properties mechanisms silencers remain largely unknown. By an unbiased genome-wide functional screen Drosophila melanogaster S2 cells, we discover a class that bind one three factors (TFs) are generally not included chromatin-defined CRE catalogs as they mostly lack detectable accessibility. The silencer-binding TF CG11247, which term Saft, safeguards cell fate decisions vivo functions via highly conserved domain zinc-finger-associated C-terminal (ZAC) corepressor G9a, independently G9a's H3K9-methyltransferase activity. Overall, our identification with unexpected has important implications for understanding future study repressive CREs, well annotation animal genomes.

Язык: Английский

Setting the stage for development: the maternal-to-zygotic transition in Drosophila DOI
Melissa M. Harrison, Audrey J. Marsh, Christine Rushlow

и другие.

Genetics, Год журнала: 2023, Номер unknown

Опубликована: Авг. 24, 2023

The zygote has a daunting task ahead of itself; it must develop from single cell (fertilized egg) into fully functioning adult with multitude different types. In the beginning, help its mother, in form gene products deposited egg, but eventually, rely on own resources to proceed through development. transfer developmental control mother embryo is called maternal-to-zygotic transition (MZT). All animals undergo this transition, which defined by two main processes-the degradation maternal RNAs and synthesis new zygote's genome. Here, we review regulation MZT Drosophila, given broad conservation essential process, much shared among metazoans.

Язык: Английский

Процитировано

5
The maternal-to-zygotic transition: reprogramming of the cytoplasm and nucleus DOI
Mina Kojima, Caroline Hoppe, Antonio J. Giráldez

и другие.

Nature Reviews Genetics, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 25, 2024

Язык: Английский

Процитировано

1
Mechanistic basis of gene regulation by SRCAP and H2A.Z DOI Open Access
Armelle Tollenaere, Enes Ugur, Cédric Deluz

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 7, 2024

Summary A central problem in gene regulation is to discriminate regulatory functions of chromatin composition from the one chromatin-modifying complexes. This question has been virtually unexplored context H2A.Z histone variant and its incorporation into nucleosomes by Snf2 Related CREBBP Activator Protein (SRCAP) complex. Here we combine rapid SRCAP depletion, a mutant lacking deposition activity, quantitative genome-wide approaches dissect how regulate transcription pluripotent stem cells. We find that exhibits essential H2A.Z-independent preventing DNA binding lineage-specific factors at enhancers stimulating factor promoters. In contrast, acts mainly as transcriptional repressor requires dynamic reloading throughout cell cycle. Our study demonstrates SRCAP-H2A.Z axis broadly orchestrates promote self-renewal, inhibit differentiation, maintain plasticity

Язык: Английский

Процитировано

1
Catalytic-dependent and independent functions of the histone acetyltransferase CBP promote pioneer factor-mediated zygotic genome activation DOI Creative Commons
Audrey J. Marsh, S. A. Pirogov,

Abby J. Ruffridge

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 5, 2024

Summary Immediately after fertilization the genome is transcriptionally quiescent. Maternally encoded pioneer transcription factors reprogram chromatin state and facilitate of zygotic genome. In Drosophila , initiated by factor Zelda. While Zelda-occupied sites are enriched with histone acetylation, a post-translational mark associated active cis -regulatory regions, functional relationship between Zelda acetylation in activation remained unclear. We show that Zelda-mediated recruitment acetyltransferase CBP essential for transcription. catalytic activity necessary release RNA Polymerase II (Pol II) into elongation embryonic development. However, also activates independent through Pol recruitment. Neither nor required pioneering function Our data suggest factor-mediated conserved mechanism to activate but this role separable from restructuring accessibility.

Язык: Английский

Процитировано

1
A genome-wide screen identifies silencers with distinct chromatin properties and mechanisms of repression DOI Creative Commons
Lorena Hofbauer, Lisa‐Marie Pleyer,

Franziska Reiter

и другие.

Molecular Cell, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Differential gene transcription enables development and homeostasis in all animals is regulated by two major classes of distal cis-regulatory DNA elements (CREs): enhancers silencers. Although have been thoroughly characterized, the properties mechanisms silencers remain largely unknown. By an unbiased genome-wide functional screen Drosophila melanogaster S2 cells, we discover a class that bind one three factors (TFs) are generally not included chromatin-defined CRE catalogs as they mostly lack detectable accessibility. The silencer-binding TF CG11247, which term Saft, safeguards cell fate decisions vivo functions via highly conserved domain zinc-finger-associated C-terminal (ZAC) corepressor G9a, independently G9a's H3K9-methyltransferase activity. Overall, our identification with unexpected has important implications for understanding future study repressive CREs, well annotation animal genomes.

Язык: Английский

Процитировано

1