London calling: The 5th UK Cellular Microbiology Network Meeting DOI Creative Commons
Serge Mostowy, Charlotte Odendall, Daniel Humphreys

и другие.

Molecular Microbiology, Год журнала: 2023, Номер 120(6), С. 906 - 909

Опубликована: Ноя. 16, 2023

The UK Cellular Microbiology Network Meeting started in 2019 with the vision of supporting and enhancing cellular microbiology community Europe. Since its inception, network meeting has emerged as an important platform for infection cell biologists to inspire transformative collaborations novel research agendas. is highly interactive focused on promoting early career researchers discovery science. fifth this series was held 19–20 June 2023 at iconic London School Hygiene & Tropical Medicine (LSHTM). Similar 2022 event Warwick (https://onlinelibrary.wiley.com/do/10.1111/mmi.0040011), spread over 2 days so that participants could maximise dynamic science downtown location. To represent ethos our community, invited speakers were Pascale Cossart (from Institut Pasteur Paris, France) Felix Randow MRC LMB Cambridge, UK). We also had a strong cohort from across Europe who presented their work (Figure 1). Following coffee welcoming remarks Serge Mostowy (LSHTM), opened keynote talk by entitled ‘How give birth star’, showcasing rise Listeria paradigm (Cossart, 2023) her decorated which recently celebrated two joined Molecular Special Issues (Buchrieser, 2020; Pizarro-Cerda, 2020). talked about career-spanning body work, including ActA actin-based motility, generation first humanised mouse model study invasion, uncovering numerous mechanisms underlying bacterial virulence. All inspired hear enthusiasm science, moving forward. Although field blooming more than ever, she advised future generations need think context evolution environment—in other words, it happening. session chaired (LSHTM) Dan Humphreys (Sheffield). Giulia Manigrasso Andrew Carter's lab, LMB) Orientia tsutsugamushi (in collaboration Jeanne Salje's CIMR) how obligate intracellular pathogen hijacks dynein motility transport autophagy evasion. next Samkeliso Lisa Blundell David Holden's Imperial College London), describing Salmonella effector SteD co-activator E3 ubiquitin ligase WWP2. last Miguel Hernandez-Gonzalez Michael Way's Francis Crick), imaging poxvirus assembly exit infected cells using state-of-the-art microscopy techniques, cryo-electron tomography (Hernandez-Gonzalez et al., 2023). Phil Elks (Sheffield) began Margarida Gomes Mostowy's LSHTM), zebrafish models decipher trained innate immunity guide vaccine studies against shigellosis (Gomes Next Thomas Burgess Elks' Sheffield) models, aiming boost immune response fungal Candida albicans via modulation hypoxia-inducible factor (HIF). Guy Pearson Jeremy Carlton's Crick Kings highlighting wide variety advanced technologies he described envelope SARS-CoV-2 (the causative agent COVID) trafficking pathway deacidify lysosomes. This followed flash talks selected submitted abstracts researchers. Gizem Ozbaykal-Guler LSHTM) interdisciplinary testing role septin hetero-oligomer composition cage entrapment Shigella. Keith Egger Charlotte Odendall's London) discussed Type III Secretion System (T3SS) SPI-2 effectors inhibition interferon signalling. Ines Diaz del Olmo Teresa Thurston's promote non-canonical reprogramming transcriptional pathways; Shan Yin James Mason's symbioses between vaginosis-associated bacteria lactobacilli. Daniel Stark Jason King's reported endosymbionts host-Mucorales interactions. Stevens Robertin his Staphylococcus aureus interactions Gram-positive pathogens. Finally, Richard Allen Meera Unnikrishnan's Warwick) S. interacts macrophages VII (T7SS). late evening ended drinks, poster electric discussion. following morning Odendall (Kings London). second speaker, Randow, delivered thought-provoking defend cytosol invasive bacteria, revealing unexpected roles RNF213 cell-autonomous (Otten 2021). Magdalena Szczesna cytosolic inhibit RNF213-mediated (Szczesna Next, Miriam Kutsch, new group leader Heinrich Heine University (Germany), guanylate binding proteins (GBPs) activate inflammasome. showcased local excellence. Lucy Thorne Greg Towers' lab convergent variants concern enhance suppression (Thorne 2022). Ann-Kathrin Reuschl Clare Jolly's enhanced evasion Omicron subvariants (Mesner In set talks, Ramon Garcia Maset Jennifer Rohn's urinary microenvironment biofilm effect antibiotic uropathogens. Janis Romanopulos into translation pleurocidin-derived antimicrobial peptide therapeutics. Ioanna Panagi molecular dissection kinase effectors. Yizhou Huang analysing antibacterial during Burkholderia. Nagisa Yoshida Naomi McGovern's Cambridge) revealed tools microbicidal capacity human placental gestation. Zoe Speirs efforts modulate therapeutic strategy tuberculosis. presentations networking, lunch coffee. final Rohn (University Enrica Pellegrino Max Gutierrez's Crick) innovative peroxisomes M. tuberculosis (Pellegrino Kathryn Wright Stefan Oehlers' Centenary Institute Australia) discovered, infection, mycobacterial infection-induced miR-126 protects host suppressing permissive (Wright J.J. Awodipe transcriptomic responses osteoblasts infection. speaker afternoon Foulkes Stephen Kaye's Liverpool) exotoxin inhibitors therapeutics, drug pipeline vitro vivo analysis. closed robust discussion prize giving, snacks. won best oral presentation Orientia, Salmonella. Find out respective interviews published alongside report (Ines, 2023; Manigrasso, are very thankful flagship sponsor Company Biologists talented been since edition 2). grateful LSHTM helping coordinate event. Mostowy: Conceptualization; writing – original draft; review editing. Odendall: Humphreys: Philip Elks: L. Rohn: authors declare no conflict interest. No or animal subjects materials used commentary. Data sharing not applicable article data created analyzed study.

Язык: Английский

An automated microscopy workflow to studyShigella–neutrophil interactions and antibiotic efficacyin vivo DOI Creative Commons
Arthur Lensen, Margarida C. Gomes, Ana T. López-Jiménez

и другие.

Disease Models & Mechanisms, Год журнала: 2023, Номер 16(6)

Опубликована: Апрель 26, 2023

Shigella are Gram-negative bacterial pathogens responsible for bacillary dysentery (also called shigellosis). The absence of a licensed vaccine and widespread emergence antibiotic resistance has led the WHO to highlight as priority pathogen requiring urgent attention. Several infection models have been useful explore process, yet we still lack information regarding events taking place in vivo. Here, using Shigella-zebrafish model high-content microscopy, develop an automated microscopy workflow non-invasively study fluorescently labelled bacteria neutrophils We apply our antibiotic-treated zebrafish demonstrate that antibiotics reduce burden not neutrophil recruitment hindbrain ventricle. discover nalidixic acid (a bactericidal antibiotic) can work with leukocytes additive manner control flexneri infection, also restrict dissemination sonnei from envision workflow, applied here Shigella-neutrophil interactions efficacy zebrafish, be innovate treatments humans.

Язык: Английский

Процитировано

4
Shigella sonnei: epidemiology, evolution, pathogenesis, resistance and host interactions DOI
Timothy A. Scott, Kate S. Baker, Caroline Trotter

и другие.

Nature Reviews Microbiology, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 27, 2024

Язык: Английский

Процитировано

1
Transcriptional profiling of zebrafish identifies host factors controlling susceptibility to Shigella flexneri DOI Creative Commons
Vincenzo Torraca, Richard White, Ian M. Sealy

и другие.

Disease Models & Mechanisms, Год журнала: 2023, Номер 17(1)

Опубликована: Дек. 22, 2023

ABSTRACT Shigella flexneri is a human-adapted pathovar of Escherichia coli that can invade the intestinal epithelium, causing inflammation and bacillary dysentery. Although an important human pathogen, host response to S. has not been fully described. Zebrafish larvae represent valuable model for studying infections in vivo. Here, we use Shigella-zebrafish infection generate mRNA expression profiles at whole-animal level. Immune response-related processes dominate signature early (6 h post-infection). Consistent with its clearance from host, late (24 post-infection) significantly changed, only small set immune-related genes remain differentially expressed, including acod1 gpr84. Using mutant lines generated by ENU, CRISPR mutagenesis F0 crispants, show acod1- gpr84-deficient are more susceptible infection. Together, these results highlight power zebrafish bacterial pathogens reveal (acutely infected) (clearing)

Язык: Английский

Процитировано

3
Factors affecting neutrophil functions during sepsis: human microbiome and epigenetics DOI Creative Commons
Yina Ma, Yu Zhao, Xin Zhang

и другие.

Journal of Leukocyte Biology, Год журнала: 2024, Номер 116(4), С. 672 - 688

Опубликована: Май 12, 2024

Abstract Sepsis is a severe disease that occurs when the body's immune system reacts excessively to infection. The response, which includes an intense antibacterial reaction, can damage its tissues and organs. Neutrophils are major components of white blood cells in circulation, play vital role innate immunity while fighting against infections, considered feature determining sepsis classification. There plethora basic research detailing neutrophil functioning, among which, study extracellular traps providing novel insights into mechanisms treatments sepsis. This review explores their functions, dysfunctions, influences context interplay between neutrophils human microbiome impact DNA methylation on function crucial areas study. interaction complex, particularly sepsis, where dysbiosis may occur. We highlight importance deciphering neutrophils’ functional alterations epigenetic features because it critical for defining endotypes opening up possibility diagnostic methods therapy. Specifically, signatures pivotal since they will provide implication method used combination with cell-free DNA. Research exploring how specific patterns neutrophils, detectable DNA, could serve as biomarkers early detection

Язык: Английский

Процитировано

0
ScRNA-seq reveals trained immunity-engaged Th17 cell activation against Edwardsiella piscicida-induced intestinal inflammation in teleost DOI
Jin Yang,

Shu Cui,

Boning Shao

и другие.

Microbiological Research, Год журнала: 2024, Номер 289, С. 127912 - 127912

Опубликована: Сен. 21, 2024

Язык: Английский

Процитировано

0
London calling: The 5th UK Cellular Microbiology Network Meeting DOI Creative Commons
Serge Mostowy, Charlotte Odendall, Daniel Humphreys

и другие.

Molecular Microbiology, Год журнала: 2023, Номер 120(6), С. 906 - 909

Опубликована: Ноя. 16, 2023

The UK Cellular Microbiology Network Meeting started in 2019 with the vision of supporting and enhancing cellular microbiology community Europe. Since its inception, network meeting has emerged as an important platform for infection cell biologists to inspire transformative collaborations novel research agendas. is highly interactive focused on promoting early career researchers discovery science. fifth this series was held 19–20 June 2023 at iconic London School Hygiene & Tropical Medicine (LSHTM). Similar 2022 event Warwick (https://onlinelibrary.wiley.com/do/10.1111/mmi.0040011), spread over 2 days so that participants could maximise dynamic science downtown location. To represent ethos our community, invited speakers were Pascale Cossart (from Institut Pasteur Paris, France) Felix Randow MRC LMB Cambridge, UK). We also had a strong cohort from across Europe who presented their work (Figure 1). Following coffee welcoming remarks Serge Mostowy (LSHTM), opened keynote talk by entitled ‘How give birth star’, showcasing rise Listeria paradigm (Cossart, 2023) her decorated which recently celebrated two joined Molecular Special Issues (Buchrieser, 2020; Pizarro-Cerda, 2020). talked about career-spanning body work, including ActA actin-based motility, generation first humanised mouse model study invasion, uncovering numerous mechanisms underlying bacterial virulence. All inspired hear enthusiasm science, moving forward. Although field blooming more than ever, she advised future generations need think context evolution environment—in other words, it happening. session chaired (LSHTM) Dan Humphreys (Sheffield). Giulia Manigrasso Andrew Carter's lab, LMB) Orientia tsutsugamushi (in collaboration Jeanne Salje's CIMR) how obligate intracellular pathogen hijacks dynein motility transport autophagy evasion. next Samkeliso Lisa Blundell David Holden's Imperial College London), describing Salmonella effector SteD co-activator E3 ubiquitin ligase WWP2. last Miguel Hernandez-Gonzalez Michael Way's Francis Crick), imaging poxvirus assembly exit infected cells using state-of-the-art microscopy techniques, cryo-electron tomography (Hernandez-Gonzalez et al., 2023). Phil Elks (Sheffield) began Margarida Gomes Mostowy's LSHTM), zebrafish models decipher trained innate immunity guide vaccine studies against shigellosis (Gomes Next Thomas Burgess Elks' Sheffield) models, aiming boost immune response fungal Candida albicans via modulation hypoxia-inducible factor (HIF). Guy Pearson Jeremy Carlton's Crick Kings highlighting wide variety advanced technologies he described envelope SARS-CoV-2 (the causative agent COVID) trafficking pathway deacidify lysosomes. This followed flash talks selected submitted abstracts researchers. Gizem Ozbaykal-Guler LSHTM) interdisciplinary testing role septin hetero-oligomer composition cage entrapment Shigella. Keith Egger Charlotte Odendall's London) discussed Type III Secretion System (T3SS) SPI-2 effectors inhibition interferon signalling. Ines Diaz del Olmo Teresa Thurston's promote non-canonical reprogramming transcriptional pathways; Shan Yin James Mason's symbioses between vaginosis-associated bacteria lactobacilli. Daniel Stark Jason King's reported endosymbionts host-Mucorales interactions. Stevens Robertin his Staphylococcus aureus interactions Gram-positive pathogens. Finally, Richard Allen Meera Unnikrishnan's Warwick) S. interacts macrophages VII (T7SS). late evening ended drinks, poster electric discussion. following morning Odendall (Kings London). second speaker, Randow, delivered thought-provoking defend cytosol invasive bacteria, revealing unexpected roles RNF213 cell-autonomous (Otten 2021). Magdalena Szczesna cytosolic inhibit RNF213-mediated (Szczesna Next, Miriam Kutsch, new group leader Heinrich Heine University (Germany), guanylate binding proteins (GBPs) activate inflammasome. showcased local excellence. Lucy Thorne Greg Towers' lab convergent variants concern enhance suppression (Thorne 2022). Ann-Kathrin Reuschl Clare Jolly's enhanced evasion Omicron subvariants (Mesner In set talks, Ramon Garcia Maset Jennifer Rohn's urinary microenvironment biofilm effect antibiotic uropathogens. Janis Romanopulos into translation pleurocidin-derived antimicrobial peptide therapeutics. Ioanna Panagi molecular dissection kinase effectors. Yizhou Huang analysing antibacterial during Burkholderia. Nagisa Yoshida Naomi McGovern's Cambridge) revealed tools microbicidal capacity human placental gestation. Zoe Speirs efforts modulate therapeutic strategy tuberculosis. presentations networking, lunch coffee. final Rohn (University Enrica Pellegrino Max Gutierrez's Crick) innovative peroxisomes M. tuberculosis (Pellegrino Kathryn Wright Stefan Oehlers' Centenary Institute Australia) discovered, infection, mycobacterial infection-induced miR-126 protects host suppressing permissive (Wright J.J. Awodipe transcriptomic responses osteoblasts infection. speaker afternoon Foulkes Stephen Kaye's Liverpool) exotoxin inhibitors therapeutics, drug pipeline vitro vivo analysis. closed robust discussion prize giving, snacks. won best oral presentation Orientia, Salmonella. Find out respective interviews published alongside report (Ines, 2023; Manigrasso, are very thankful flagship sponsor Company Biologists talented been since edition 2). grateful LSHTM helping coordinate event. Mostowy: Conceptualization; writing – original draft; review editing. Odendall: Humphreys: Philip Elks: L. Rohn: authors declare no conflict interest. No or animal subjects materials used commentary. Data sharing not applicable article data created analyzed study.

Язык: Английский

Процитировано

0