Plant Biotechnology Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 22, 2024
The
protein
crops
known
as
lupins
have
been
bred
to
accumulate
low
levels
of
antinutritional
alkaloids,
neglecting
their
potential
sources
valuable
metabolites.
Here,
we
engineered
narrow-leafed
lupin
(NLL)
large
amounts
a
single
alkaloid
industrial
interest
called
(-)-sparteine.
While
(-)-sparteine
is
recognized
key
auxiliary
molecule
in
chiral
synthesis,
its
variable
price
and
limited
availability
prevented
large-scale
use.
We
identified
two
enzymes
that
initiate
the
conversion
variety
alkaloids
accumulating
NLL.
first
one
cytochrome
P450
monooxygenase
belonging
family
71
(CYP71D189),
second
short-chain
dehydrogenase/reductase
(SDR1).
screened
non-GMO
NLL
mutant
library
isolated
knockout
CYP71D189.
displayed
an
altered
metabolic
profile
where
accounted
for
96%
content
seeds
(GC-MS
basis).
from
was
enantiomerically
pure
(99%
enantiomeric
excess).
Apart
profile,
did
not
any
noticeable
phenotype.
Our
work
demonstrates
precursor
most
QAs
expands
current
uses
crop.
Scientific Reports,
Год журнала:
2025,
Номер
15(1)
Опубликована: Янв. 21, 2025
Abstract
White
lupin
(
Lupinus
albus
L.)
is
an
ancient
grain
legume
that
still
undergoing
improvement
of
domestication
traits,
including
vernalization-responsiveness,
providing
frost
tolerance
and
preventing
winter
flowering
in
autumn-sowing
agriculture,
vernalization-independence,
conferring
drought
escape
by
rapid
spring-sowing.
A
recent
genome-wide
association
study
highlighted
several
loci
significantly
associated
with
the
most
contrasting
phenotypes,
deletions
promoter
FLOWERING
LOCUS
T
homolog,
LalbFTc1
,
some
DArT-seq/silicoDArT
loci.
The
present
aimed
to
develop
validate
a
versatile
PCR
marker
array
enabling
molecular
selection
spring-
winter-type
white
ecotypes.
Candidate
DArT-seq
silicoDArT
were
transformed
into
cleaved
amplified
polymorphic
sequence
(CAPS)
or
derived
CAPS
markers.
Developed
markers,
together
those
previously
published
for
INDELs
quantitative
trait
from
linkage
maps,
implemented
screening
germplasm
panel
subjected
2-year
phenotyping
phenology
traits.
Three
DArT-seq,
two
seven
INDEL
markers
positively
validated,
constituting
convenient
PCR-based
assay
accurate
reselection
towards
early
thermoneutrality
late
as
well
tracking
high
genetic
phenotypic
diversity
within
landraces,
revealed
study.
Plants,
Год журнала:
2025,
Номер
14(6), С. 914 - 914
Опубликована: Март 14, 2025
The
higher
adaptation
of
landraces
to
local
agroclimatic
conditions
resulting
from
natural
and
moderate
artificial
selection
by
farmers
within
specific
environments
makes
them
a
crucial
source
alleles
genotypes
for
cultivation
breeding
programs.
Unlike
modern
cultivars,
which
have
been
developed
under
more
intense
selective
pressures,
exhibit
broader
genetic
base
that
has
documented
in
landrace
collections
many
crops.
This
review
provides
an
overview
the
importance
resource
valorisation
legume
species,
focusing
on
cultivated
species
Lupinus
genus,
particularly
white
lupin
(Lupinus
albus).
On
one
hand,
legumes,
including
Lupins,
are
considered
alternative
protein
framework
sustainable
agriculture.
other
they
often
neglected
terms
efforts,
despite
receiving
increasing
attention
recent
years.
Here,
we
also
report
latest
advances
development
genomic
tools,
such
as
novel
pangenome
identification
markers
loci
target
traits,
tolerance
alkaline
soils,
can
effectively
support
albus,
especially
introgression
desirable
locally
adapted
varieties.
Plant Biotechnology Journal,
Год журнала:
2024,
Номер
22(11), С. 3051 - 3053
Опубликована: Июль 9, 2024
In
nature,
genetic
variation
occurs
in
every
population
and
results
the
evolution
of
a
diversity
new
properties,
some
which
promote
survival
species.
To
accelerate
nature's
based
on
diversity,
plant
breeders
may
induce
additional
mutations
to
raise
number
variations
increasing
chances
obtain
varieties
with
desired
traits
like
improved
nutritive
quality,
yields
resilience
biotic
abiotic
stress
factors.
Induced
mutagenesis
chemical
mutagens
is
considered
non-GM
has
been
used
barley
(Hordeum
vulgare)
for
decades
(Hansson
et
al.,
2024).
Reverse
techniques
including
TILLING
(Targeting
Local
Lesions
Genomes)
screening
methodology
more
recently
TILLING-by-sequencing
spinoffs
are
tools
identify
individual
variants
valuable
genomic
alterations.
However,
these
hampered
by
low
mutation
capacity.
PCR-based
technique
designed
detect
mismatched
single
nucleotides
target
gene.
2023,
Szarejko
her
research
group
Poland
published
thorough
overview
success
stories
within
last
20
years
(Szurman-Zubrzycka
2023)
description
populations
different
cultivars
landraces
obtained
following
(Figure
1a).
The
sizes
range
between
1372
9600
variants.
frequencies
individually
chosen
dose-dependent
(1/154–1/2500
Kbp).
When
multiplied
(#
individuals
×
per
individual),
total
present
ranges
10
100
million
This
sound
lot,
but
genome
size
around
4300
Mbp
(here,
RGT
Planet;
Jayakodi
2020),
less
than
2%
entire
mutated.
severely
reduces
possibility
find
populations.
FIND-IT
technology
approach
overriding
constraints.
was
Science
Advances
2022
1b)
(Knudsen
2022)
provides
an
agile
high-throughput
screen
unprecedented
large
chemically
induced
variant
combines
systematic
sample
pooling
splitting
high-sensitivity,
droplet
digital
PCR
(ddPCR)–based
genotyping
targeted
identification
at
single-nucleotide
resolution.
ddPCR
1000-fold
sensitive
conventional
PCR.
applicable
any
living
organism
that
can
be
grown
field
or
culture.
experimental
outlined
detail
Knudsen
illustrated
schematically
Figure
1b.
total,
500
000
plants
today
available
screening.
were
also
developed
other
crops
microorganisms
using
sodium
azide
ethyl
methanesulphonate
(EMS)
as
mutagens.
Thus
key
sweet
seeds
white
lupin
(Mancinotti
2023),
eliminate
presence
anti-nutritional
saponins
quinoa
(Trinh
2024),
improve
phosphate
bioavailability
grain
(Madsen
avoid
hydroxynitrile
glucoside-derived
formation
pro-carcinogen
carbamate
whisky
production
S1;
Jørgensen
2024)
modify
flavour
profiles
Saccharomyces
species
use
industrial
brewing
(Stovisek
pipeline
knowledge
mutant
load
spectrum
doses
monitored
whole
sequencing
1b;
2022).
introduced
found
equally
distributed
over
seven
chromosomes
higher
observed
increased
mutagen
doses.
Thus,
libraries
generated
purposes:
Medium
(e.g.
1.7
mm
treatment
average
14
770
SNPs
plant)
gene-function
analyses
0.3
5565
breeding
whole-genome
documents
15%
transversions
85%
transitions
preference
C
>
T
G
A
1c).
Planet
genome,
1900
Mb
C's
G's.
Upon
dose,
randomly
positioned
each
8000
will
transitions.
Using
technology,
library
collection
350
now
analysed
containing
approximately
=
2
800
1b).
Because
only
harbours
nucleotides,
this
means
virtually
all
putative
sites
have
saturated.
saturated,
their
status
shifts
from
being
random
become
defined
distinct
one
collection.
Accordingly,
grains
carrying
specific
SNP
gene
identified
many
pools
TaqMan™
assay
You
know
there
just
FIND-IT.
Compared
existing
cereal
resources
relation
natural
pan-genome
accession
panel,
off-target
pressure
efficiently
reduced
backcrossing
parent
direct
crosses
elite
commercial
programmes.
context,
it
noticed
would
typically
adjusted
comparable
classical
step
two
parents
naturally
genomes
As
proof
principle,
we
demonstrated
efficiency
isolating
knockout
lines
dozen
amino
acid
exchanges
miRNA
promoter
S2;
Taking
advantage
fact
non-GMO
method,
data
directly
verified
growing
2022),
important
requirement
validate
crop
(Khaipho-Burch
2023).
current
development
high-quality
allows
strategies
highly
customized.
While
static
regarding
variant,
CRISPR
technologies
still
facing
multiple
challenges
field-applicable
(Cardi
stays
agile,
offers
high
flexibility
high-throughput,
compatible
today.
regularly
updated
lines,
customized
winter
versus
spring
libraries)
while
sensitivity
keeps
competitive.
isolated
line
load,
original
implemented
protocols
lose
during
yield
cycles
providing
fast
market
rollout
novel
traits.
We
thank
Carlsberg
Foundation
funding
support:
grant:
CF14-0461
(to
B.L.M.)
CF15-0236
B.S.).
authors
not
declared
conflict
interest.
Data
sharing
article
no
datasets
study.
S1.
Disentangling
glucoside
biosynthesis
metabolon
access
malting
barleys
free
production.
S2.
Barley
selected
agronomically
evaluated.
Please
note:
publisher
responsible
content
functionality
supporting
information
supplied
authors.
Any
queries
(other
missing
content)
should
directed
corresponding
author
article.
ACS Food Science & Technology,
Год журнала:
2025,
Номер
unknown
Опубликована: Янв. 22, 2025
Recently,
it
was
shown
that
food
products
made
from
the
seeds
of
Lupinus
species
contain
quinolizidine
alkaloids
in
range
several
mg/kg.
Some
these
compounds
are
not
very
well
characterized
regard
to
their
toxic
mechanism
action.
Specifically,
we
identified
data
gaps
on
genotoxicity
and
mutagenicity
contaminants,
which
aimed
close.
Therefore,
applied
a
basic
test
battery
vitro
assays
including
bacterial
reverse
mutation
assay
micronucleus
study
genotoxic
potential
five
selected
lupine
angustifoline,
lupanine,
13-hydroxylupanine,
lupinine,
sparteine.
We
found
none
showed
any
cytotoxic,
genotoxic,
or
mutagenic
vitro.
Food Additives & Contaminants Part A,
Год журнала:
2025,
Номер
unknown, С. 1 - 16
Опубликована: Апрель 28, 2025
Lupin
is
a
pulse
crop
valued
for
its
protein
content
and
potential
as
functional
ingredient,
e.g.
in
gluten-free
foods.
The
presence
of
quinolizidine
alkaloids
(QAs)
phomopsins
(PHO)
has
raised
concerns,
yet
information
regarding
their
occurrence
remains
limited.
A
two-year
screening
QAs
PHO
lupin-containing
foods
available
on
the
Belgian
market
was
conducted,
based
study
risk-based
sampling
plan
(n
=
339).
UHPLC-MS/MS
method
validated
simultaneous
quantification
seven
(i.e.
sparteine,
lupanine,
lupinine,
13α-OH-lupanine,
angustifoline,
multiflorine
albine)
phomopsin
relevant
matrices.
not
detected
any
samples
(
Current Opinion in Biotechnology,
Год журнала:
2024,
Номер
88, С. 103147 - 103147
Опубликована: Июнь 3, 2024
Plant
natural
products
have
been
an
important
source
of
medicinal
molecules
since
ancient
times.
To
gain
access
to
the
whole
diversity
these
for
pharmaceutical
applications,
it
is
understand
their
biosynthetic
origins.
Whilst
co-expression
a
reliable
tool
identifying
gene
candidates,
variety
complementary
methods
can
aid
in
screening
or
refining
candidate
selection.
Here,
we
review
recently
employed
plant
pathway
discovery
approaches,
and
highlight
future
directions
field.
The Plant Journal,
Год журнала:
2024,
Номер
unknown
Опубликована: Сен. 12, 2024
SUMMARY
Lupinus
mutabilis
is
an
under‐domesticated
legume
species
from
the
Andean
region
of
South
America.
It
belongs
to
New
World
lupins
clade,
which
groups
several
lupin
displaying
large
genetic
variation
and
adaptability
highly
different
environments.
L.
attracting
interest
as
a
potential
multipurpose
crop
diversify
European
supply
plant
proteins,
increase
agricultural
biodiversity,
fulfill
bio‐based
applications.
This
study
reports
first
high‐quality
genome
assembly,
also
sequenced
assembly
species.
Through
comparative
genomics
phylogenetics,
evolution
within
legumes
described,
highlighting
both
genomic
similarities
patterns
specific
,
potentially
linked
environmental
adaptations.
Furthermore,
was
used
genetics
underlying
important
traits
for
establishment
novel
crop,
including
protein
quinolizidine
alkaloids
contents
in
seeds,
classic
resistance
genes,
properties
mycorrhiza‐related
genes.
These
analyses
pointed
out
copy
number
variation,
differential
gene
contexts,
family
expansion
through
tandem
duplications
likely
drivers
diversity
observed
these
between
other
legumes.
Overall,
will
be
valuable
resource
conduct
research
enable
genomic‐based
breeding
approaches
turn
into
crop.
Sparteine
is
a
plant-derived
alkaloid
widely
known
for
its
utility
as
chiral
ligand
in
asymmetric
synthesis
1-3
.
However,
variable
market
price
and
availability
have
failed
to
meet
the
demand
cheap
reliable
product
4-6
naturally
synthesized
by
sub-group
of
legume
plants,
which
typically
accumulate
complex
mixtures
closely
related
alkaloids.
Here,
we
identified
two
enzymes
from
narrow-leafed
lupin
(NLL,
L.
angustifolius
)
that
can
sequentially
oxidize
(−)-sparteine
(+)-lupanine.
The
first
enzyme
cytochrome
P450
monooxygenase
belonging
family
71
(CYP71D189)
second
one
short-chain
dehydrogenase/reductase
(SDR1).
We
also
screened
non-GMO
NLL
mutant
library
isolated
knockout
CYP71D189.
displayed
an
altered
metabolic
profile
where
accounted
96%
content
seeds
(GC-MS
basis).
was
enantiomerically
pure
(99%
ee
).
Apart
profile,
did
not
any
noticeable
phenotype.
Our
work
demonstrates
precursor
most
QAs
provides
convenient
source
this
valuable
compound
academia
industry.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 4, 2024
Abstract
Piperidine
alkaloids
in
plants
derive
from
lysine
via
Δ
1
-piperideine.
We
investigated
the
alkaloid
biosynthesis
Flueggea
suffruticosa
,
a
plant
that
produces
diverse
piperidine
alkaloids,
including
securinine.
Until
now,
pathways
leading
to
formation
of
-piperideine
have
involved
two
enzymatic
steps:
decarboxylase
and
an
oxidase,
proceeding
free
cadaverine.
discovered
synthase
(PS),
PLP-dependent
enzyme
forms
directly
oxidative
deamination.
demonstrate
PS
catalyses
this
transformation
non-symmetric
manner,
show
it
is
positioned
within
group
III
family,
which
typically
associated
with
prokaryotes.
also
identified
paralog
gene
exhibiting
activity.
This
work
delineates
first
step
securinine
as
unprecedented
deamination
lysine.
It
marks
identification
eukaryotic
resolves
longstanding
question
nitrogen
incorporation
alkaloids.