Engineered Bio‐Heterojunction for Effective Treatment of Colorectal Cancer Through Intratumoral Bacteria Scavenging and Autophagy Regulation

Advanced Functional Materials, Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

Abstract Intratumor bacteria, a critical component of the tumor microenvironment, can facilitate growth and contribute to development resistance chemotherapeutic agents. The agents not only induce protective autophagy in tumors, contributing drug resistance, but also exert minimal effects on bacteria within tumor, thereby potentially accelerating recurrence. To address this tough issue, vermiculite/copper telluride bio‐heterojunctions (VIC bio‐HJs) have been developed enhance eradicate obtain victory fight against tumors. VIC bio‐HJs are able generate substantial reactive oxygen species (ROS) via photodynamic/chemodynamic therapy, effectively eliminating by disrupting bacterial integrity impairing electron transport chains. Concurrently, increased production ROS leads decreased mitochondrial membrane potential apoptosis cells. In vivo assays sequencing analysis confirm that excellent bacteria‐killing ability while being excessive cells, ultimately leading more cell death. Such work proposes strategy for designing capable eradication removal, offering promising approach improve efficacy bacterial‐infiltrated therapy.

Язык: Английский

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Phenylboronic Acid-Modified Carbon Dot-Proteinase K Nanohybrids for Enhanced Photodynamic Therapy Against Bacterial Biofilm Infections

Acta Biomaterialia, Год журнала: 2025, Номер unknown

Опубликована: Янв. 1, 2025

Язык: Английский

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NIR photo-responsive injectable chitosan/hyaluronic acid hydrogels with controlled NO release for the treatment of MRSA infections

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140304 - 140304

Опубликована: Янв. 1, 2025

Язык: Английский

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Arginine and colorectal cancer: Exploring arginine-related therapeutic strategies and novel insights into cancer immunotherapies

International Immunopharmacology, Год журнала: 2025, Номер 148, С. 114146 - 114146

Опубликована: Янв. 28, 2025

Язык: Английский

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On-demand celastrol delivery by hyaluronic acid-porphyrinic metal-organic frameworks for synergistic sonodynamic/pharmacological antibacterial therapy

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140421 - 140421

Опубликована: Янв. 1, 2025

Язык: Английский

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A spatial confinement biological heterogeneous cascade nanozyme composite hydrogel combined with nitric oxide gas therapy for enhanced treatment of psoriasis and diabetic wound

Chemical Engineering Journal, Год журнала: 2025, Номер unknown, С. 160629 - 160629

Опубликована: Фев. 1, 2025

Язык: Английский

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The Purification and Characterization of a Novel Neutral Protease from Volvariella volvacea Fruiting Bodies and the Enzymatic Digestion of Soybean Isolates

Journal of Fungi, Год журнала: 2025, Номер 11(3), С. 190 - 190

Опубликована: Март 1, 2025

A novel protease was isolated from the fruiting bodies of straw mushroom Volvariella volvacea. The purified 13.48-fold using a series techniques, including ammonium sulfate precipitation, ultrafiltration, diethylaminoethyl fast-flow (DEAE FF) ion-exchange chromatography, and Superdex 75 gel filtration resulting in specific enzyme activity 286.82 U/mg toward casein as substrate. Sodium dodecyl sulfate–polyacrylamide electrophoresis (SDS-PAGE) analysis revealed that had molecular weight 24 kDa. exhibited optimal at pH 7 50 °C, showing sensitivity to alkaline conditions instability elevated temperatures. presence Ca2+ significantly enhanced activity, whereas Ni2+ Cu2+ exerted strong inhibitory effects, with other metal ions weak inhibition. β-mercaptoethanol, Tween-80, Triton X-100 more pronounced PMSF, EDTA, CTAB weaker effects. Michaelis constant (Km) maximum velocity (Vm) were determined be 1.34 g/L 3.45 μg/(mL·min), respectively. greater degree enzymatic degradation soybean-isolate protein (7.58%) compared trypsin (5.24%), product containing high percentage medicinal amino acids (73.54%), particularly phenylalanine (Phe) arginine (Arg), suggesting their enzyme’s active site. These findings suggest V. volvacea holds promising potential for applications food industry, hydrolysate production flavor enhancement.

Язык: Английский

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NIR light-driven nanomotor with cascade photodynamic therapy for MRSA biofilm eradication and diabetic wound healing

Theranostics, Год журнала: 2025, Номер 15(8), С. 3474 - 3489

Опубликована: Фев. 24, 2025

Background: Diabetic wounds infected with methicillin-resistant Staphylococcus aureus (MRSA) are challenging to heal due biofilm formation, which impairs conventional antibiotics limited penetration and severe side effects. Near-infrared (NIR)-driven nanomotors autonomous motion photothermal effects show promise for antibacterial therapy but often lack targeted specificity. Lysostaphin (Ly), an enzyme targeting bacterial cell walls, offers excellent potential against drug-resistant MRSA. Methods: A novel NIR-driven CSIL nanomotor has fabricated by co-loading indocyanine green (ICG) lysostaphin onto spinous yolk-shell structured C/SiO2@C nanoparticles. The motion, penetration, efficacy of evaluated in vitro, while their eradication wound healing performance assessed MRSA-infected diabetic mouse model using a cascade photodynamic (CPDT) strategy. Results: exhibit properties MRSA-targeting They can effectively eradicate MRSA biofilms both vitro vivo, suppress virulence biofilm-related genes, thus promoting shaping microenvironment dominated M2 macrophages. CPDT strategy is able avoid excessive ROS production thermal damage, enabling safe effective therapy. Conclusion: nanomotors, integrated photothermal, photodynamic, properties, represent novel, efficient approach wounds, offering significant advantages over antibiotics.

Язык: Английский

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Arginine-terminated egg white peptide Leu-Phe-Arg (LFR) repairs intestinal barrier damage through the PI3K/AKT/mTOR/p70s6k signaling pathway: An analysis combined transcriptomics and proteomics

Food Bioscience, Год журнала: 2025, Номер unknown, С. 106502 - 106502

Опубликована: Апрель 1, 2025

Язык: Английский

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Cascade reaction hydrogel with enzyme-catalyzed endogenous glucose for diabetic wound healing

Journal of Colloid and Interface Science, Год журнала: 2025, Номер 693, С. 137616 - 137616

Опубликована: Апрель 16, 2025

Язык: Английский

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