Inter-laboratory harmonization of microsphere immunoassays for SARS-CoV-2 antibody detection in dried blood spots and oral fluids DOI Creative Commons
Kate L. DeRosa, Nora Pisanic, Kate Kruczynski

и другие.

Опубликована: Окт. 22, 2024

ABSTRACT Dried blood spots (DBS) and oral fluids (OF) are easily attainable biospecimen types that have enabled population scale antibody monitoring for SARS-CoV-2 exposure vaccination. However, the degree to which two different can be used interchangeably remains unclear. To begin address this question, we generated contrived DBS (cDBS) OF (cOF) from serum panels infected, vaccinated, uninfected individuals. The samples were evaluated using multiplexed microsphere immunoassays (MIAs) at institutions. Intra-laboratory tests revealed near perfect agreement between cDBS cOF N S antigens, as evidenced by κ = 0.97-1 98%-100% agreement. Inter-laboratory comparisons equally robust both (κ 0.94-0.96; 97.5%-98 % agreement) 0.98 -1.0; 99.0%-100%). Furthermore, assays transferred labs, including methods reagents, a subset of (n 52) tested. Qualitative concordance remained high 0.94-1.0; 97.5%-100% agreement), confirming integrity is retained upon transfer. In summary, our results provide evidence across laboratories institutions qualitative assessment determinations.

Язык: Английский

Inter-laboratory harmonization of microsphere immunoassays for SARS-CoV-2 antibody detection in contrived dried blood spots and oral fluids DOI Creative Commons
Kate L. DeRosa, Nora Pisanic, Kate Kruczynski

и другие.

Microbiology Spectrum, Год журнала: 2025, Номер unknown

Опубликована: Март 25, 2025

ABSTRACT Dried blood spots (DBS) and oral fluids (OF) are easily attainable biospecimen types that enabled population-scale antibody monitoring for SARS-CoV-2 exposure vaccination. However, the degree to which two different can be used interchangeably remains unclear. To address this question, we generated contrived DBS (cDBS) OF (cOF) from serum panels of SARS-CoV-2-infected, vaccinated, uninfected individuals. The samples were evaluated using multiplexed microsphere immunoassays (MIAs) at institutions. Intra-laboratory tests revealed near-perfect agreement between cDBS cOF N S antigens, as evidenced by κ = 0.97–1 98%–100% agreement. Interlaboratory comparisons equally robust both (κ 0.94–0.96; 97.5%–98 % agreement) 0.98–1.0; 99.0%–100%). Furthermore, assays transferred labs, including methods reagents, a subset ( n 52) tested. Qualitative concordance remained high 0.94–1.0; 97.5%–100% agreement), confirming integrity is retained upon transfer. In summary, our results provide evidence across laboratories institutions qualitative assessment determinations. IMPORTANCE establishment common research clinical laboratory methodologies levels in paramount importance estimating immunity COVID-19 infections communities populations. report, multiplex immunoassay protocols enable readily types, namely, dried (OF), employed titers virus-neutralizing potential.

Язык: Английский

Процитировано

0
Inter-laboratory harmonization of microsphere immunoassays for SARS-CoV-2 antibody detection in dried blood spots and oral fluids DOI Creative Commons
Kate L. DeRosa, Nora Pisanic, Kate Kruczynski

и другие.

Опубликована: Окт. 22, 2024

ABSTRACT Dried blood spots (DBS) and oral fluids (OF) are easily attainable biospecimen types that have enabled population scale antibody monitoring for SARS-CoV-2 exposure vaccination. However, the degree to which two different can be used interchangeably remains unclear. To begin address this question, we generated contrived DBS (cDBS) OF (cOF) from serum panels infected, vaccinated, uninfected individuals. The samples were evaluated using multiplexed microsphere immunoassays (MIAs) at institutions. Intra-laboratory tests revealed near perfect agreement between cDBS cOF N S antigens, as evidenced by κ = 0.97-1 98%-100% agreement. Inter-laboratory comparisons equally robust both (κ 0.94-0.96; 97.5%-98 % agreement) 0.98 -1.0; 99.0%-100%). Furthermore, assays transferred labs, including methods reagents, a subset of (n 52) tested. Qualitative concordance remained high 0.94-1.0; 97.5%-100% agreement), confirming integrity is retained upon transfer. In summary, our results provide evidence across laboratories institutions qualitative assessment determinations.

Язык: Английский

Процитировано

0