Ubiquitous purine sensor modulates diverse signal transduction pathways in bacteria DOI Creative Commons
Tino Krell, Elizabet Monteagudo‐Cascales, Vadim M. Gumerov

и другие.

Research Square (Research Square), Год журнала: 2023, Номер unknown

Опубликована: Ноя. 16, 2023

Abstract Purines and their derivatives are key molecules for controlling intracellular energy homeostasis nucleotide synthesis. In eukaryotes, including humans, purines also act as signaling that mediate extracellular communication control cellular processes, such proliferation, migration, differentiation, apoptosis. However, the role of in bacteria is largely unknown. Here, by combining structural sequence information, we define a purine-binding motif, which present sensor domains thousands bacterial receptors modulate motility, gene expression, metabolism second messenger turnover. The screening compound libraries microcalorimetric titrations selected validated ability to specifically bind purine derivatives. physiological relevance sensing was demonstrated system modulates c-di-GMP levels.

Язык: Английский

Engineering two-component systems for advanced biosensing: From architecture to applications in biotechnology DOI

Wenyan Cao,

Chao Huang, Xuan Zhou

и другие.

Biotechnology Advances, Год журнала: 2024, Номер 75, С. 108404 - 108404

Опубликована: Июль 11, 2024

Язык: Английский

Процитировано

2
Dimerization Rules of Mammalian PAS Proteins DOI

Brenda L. Rojas,

Emmanuel Vazquez-Rivera,

Carrie L. Partch

и другие.

Journal of Molecular Biology, Год журнала: 2023, Номер 436(3), С. 168406 - 168406

Опубликована: Дек. 16, 2023

Язык: Английский

Процитировано

3
Ubiquitous purine sensor modulates diverse signal transduction pathways in bacteria DOI Open Access
Elizabet Monteagudo‐Cascales, Vadim M. Gumerov, Matilde Fernández

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Окт. 26, 2023

Abstract Purines and their derivatives are key molecules for controlling intracellular energy homeostasis nucleotide synthesis. In eukaryotes, including humans, purines also act as signaling that mediate extracellular communication control cellular processes, such proliferation, migration, differentiation, apoptosis. However, the role of in bacteria is largely unknown. Here, by combining structural sequence information, we define a purine-binding motif, which present sensor domains thousands bacterial receptors modulate motility, gene expression, metabolism second messenger turnover. The screening compound libraries microcalorimetric titrations selected validated ability to specifically bind purine derivatives. physiological relevance sensing was demonstrated system modulates c-di-GMP levels.

Язык: Английский

Процитировано

2
Mechanisms of PAS Domain Signalling, from Sensing Varied Small Molecules and Peptides to Approved Pharmaceuticals and Use in Optogenetics DOI
Sepideh Khorasanizadeh, Kevin H. Gardner

Journal of Molecular Biology, Год журнала: 2024, Номер 436(3), С. 168457 - 168457

Опубликована: Янв. 24, 2024

Язык: Английский

Процитировано

0
Chlorpromazine inhibits EAG1 channels by altering the coupling between the PAS, CNBH and pore domains DOI
Mahdi Ghorbani, Zejun Wang, Xi Chen

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Фев. 26, 2024

ABSTRACT EAG1 depolarization-activated potassium selective channels are important targets for treatment of cancer and neurological disorders. formed by a tetrameric subunit assembly with each containing an N-terminal Per-Arnt-Sim (PAS) domain C-terminal cyclic nucleotide-binding homology (CNBH) domain. The PAS CNBH domains from adjacent subunits interact form intracellular ring that regulates the channel gating, including movement voltage sensor (VSD) closed to open states. Small molecule ligands can inhibit binding their domains. However, allosteric pathways this inhibition not known. Here we show chlorpromazine, small binder, alters interactions between decreases coupling pore channel, while having little effect on VSD In addition, chlorpromazine did alter Cole-Moore shift characteristic channels, further indicating has no deep opened Our study provides framework understanding global regulation binders.

Язык: Английский

Процитировано

0
Loss of LasR function leads to decreased repression ofPseudomonas aeruginosaPhoB activity at physiological phosphate concentrations DOI Creative Commons
Amy Conaway, Igor Todorovic, Dallas L. Mould

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Март 27, 2024

While the

Язык: Английский

Процитировано

0
Previously uncharacterized aliphatic amino acid positions modulate the apparent catalytic activity of the EAL domain of ZMO_1055 and other cyclic di-GMP specific EAL phosphodiesterases DOI Creative Commons

Lian-Ying Cao,

Yongfu Yang, Feng‐Wu Bai

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июнь 21, 2024

Abstract The ubiquitous second messenger cyclic di-GMP is the most abundant diffusible nucleotide signalling system in bacteria deciding life style transition between sessility and motility. GGDEF diguanylate cyclases EAL phosphodiesterases conventionally direct turnover of this signaling molecule. Thereby, those domains are subject to micro- macroevolution with evolutionary forces that promote alterations these proteins currently mostly unknown. While highly conserved signature amino acids involved divalent ion binding catalysis equally as signal transduction modules have been readily identified, more subtle acid substitutions modulate catalytic activity rarely recognized their molecular mechanism characterized. Our previous work identified A526V substitution be downregulation apparent Zymomonas mobilis ZM4 PAS-GGDEF-EAL ZMO1055 phosphodiesterase leading a self-flocculation phenotype mediated by elevated production exopolysaccharide cellulose Z. ZM401. As A526 located at position has previously not affect domain, we further investigated mechanisms functional conservation substitution. Using number model systems, our results indicate alanine 526 homologs beyond mutation alter subgroups domains. Thus hypothesize single lead amplify output thus significantly contribute flexibility adaptability network. In context, seems current target.

Язык: Английский

Процитировано

0
Structures of the multi-domain oxygen sensor DosP: remote control of a c-di-GMP phosphodiesterase by a regulatory PAS domain DOI Creative Commons
Wenbi Shcherbakov-Wu, Pankaj Kumar, Chad A. Brautigam

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 24, 2024

The heme-based direct oxygen sensor DosP degrades c-di-GMP, a second messenger nearly unique to bacteria. In stationary phase

Язык: Английский

Процитировано

0
Apusomonad photophobic behavior highlights cytoskeletal responses to blue light in early eukaryotes DOI Creative Commons
Aika Shibata, Chiaki Yamamoto, Ryuji Yanase

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Сен. 3, 2024

Abstract Light response is a fundamental characteristic of eukaryotic organisms, both unicellular and multicellular. However, no photoresponse has previously been reported in Apusomonadida, group small, free-living biflagellates phylogenetically positioned as sister to Opisthokonta (animals, fungi, their relatives). Apusomonads are thus crucial for understanding the evolution opisthokonts. Here, we report first time an avoidance blue light apusomonad Podomonas kaiyoae . This accompanied by increase gliding velocity, transient changes flagellar waveforms, alterations cell shape. Dynamic contraction induced intracellular free Ca2+ inhibited either dynein inhibitor or actin-disrupting drug. These findings suggest that photophobic behavior relies on cytoskeletal responses mediated dynein/tubulin myosin/actin systems, which were acquired early evolution. The dominance posterior flagellum cilia-driven directional further supports phylogenetic placement apusomonads opisthokonts rather than other lineages.

Язык: Английский

Процитировано

0
Structure and distribution of sensor histidine kinases in the fungal kingdom DOI
Sara Mina, Anaïs Hérivaux, Hajar Yaakoub

и другие.

Current Genetics, Год журнала: 2024, Номер 70(1)

Опубликована: Сен. 14, 2024

Язык: Английский

Процитировано

0