Cell Reports, Год журнала: 2024, Номер 43(12), С. 115025 - 115025
Опубликована: Ноя. 30, 2024
Язык: Английский
Science Advances, Год журнала: 2025, Номер 11(9)
Опубликована: Фев. 26, 2025
Naturally occurring extracellular vesicles (EVs) and synthetic nanoparticles like liposomes have revolutionized precision diagnostics medicine. EVs excel in biocompatibility cell targeting, while offer enhanced drug loading capacity scalability. The clinical translation of is hindered by challenges including low yield heterogeneity, whereas face rapid immune clearance limited targeting efficiency. To bridge these gaps, biomimetic (SVs) emerged as innovative platforms, combining the advantageous properties liposomes. This review emphasizes critical aspects EV biology, such mechanisms EV-cell interaction source-dependent functionalities modulation, tissue regeneration, informing SV engineering. We reviewed a broad array SVs, with focus on lipid bilayered functionalized proteins. These include cell-derived nanovesicles, protein-functionalized liposomes, hybrid vesicles. By addressing current highlighting opportunities, this aims to advance SVs for transformative biomedical applications.
Язык: Английский
Процитировано
4
Cancer Biology & Therapy, Год журнала: 2025, Номер 26(1)
Опубликована: Янв. 26, 2025
Adaptive immune resistance in cancer describes the various mechanisms by which tumors adapt to evade anti-tumor responses. IFN-γ induction of programmed death-ligand 1 (PD-L1) was first defined and validated adaptive mechanism. The endoplasmic reticulum (ER) is central as modulatory secreted integral membrane proteins are dependent on ER. Sigma1 a unique ligand-regulated scaffolding protein enriched ER cells. PD-L1 an glycoprotein that translated into processed through cellular secretory pathway. At cell surface, checkpoint molecule binds PD-1 activated T-cells blocks immunity. can also be incorporated cell-derived extracellular vesicles (EVs), EV-associated inactivate within tumor microenvironment. Here, we demonstrate selective small inhibitor block mediated part altering incorporation EVs. inhibition blocked post-translational maturation downstream IFN-γ/STAT1 signaling. Subsequently, EVs released response stimulation were significantly less potent suppressors T-cell activation. These results suggest reducing derived suppressive EVs, may promote antitumor modulation presents novel approach regulating microenvironment content production Altogether, these data support notion play role
Язык: Английский
Процитировано
3
Chemical Engineering Journal, Год журнала: 2024, Номер 496, С. 154270 - 154270
Опубликована: Июль 24, 2024
Язык: Английский
Процитировано
9
Frontiers in Immunology, Год журнала: 2024, Номер 15
Опубликована: Авг. 1, 2024
Exosomes play a crucial role in various biological processes, such as human development, immune responses, and disease occurrence. The membrane proteins on exosomes are pivotal factors for their functionality. Currently, numerous have been identified exosome membranes, participating intercellular communication, mediating target cell recognition, regulating processes. Furthermore, from derived cancer cells can serve relevant biomarkers early diagnosis. This article provides comprehensive review of the composition diverse functions organism's Through in-depth exploration proteins, it is expected to offer essential foundations future development novel biomedical diagnostics therapies.
Язык: Английский
Процитировано
7
Journal of Biological Chemistry, Год журнала: 2025, Номер unknown, С. 108255 - 108255
Опубликована: Фев. 1, 2025
Small extracellular vesicles (sEVs) are particles secreted from cells that play vital roles both in normal physiology and human disease. sEVs highly enriched tetraspanin proteins, such as CD9 CD63, contain tetraspanin-enriched membrane microdomains involved loading of with macromolecule cargoes sEV biogenesis. However, the precise individual tetraspanins biogenesis cargo remain poorly understood. Here, we report negatively regulated CD63 trafficking to its subsequent packaging into small EVs, whereas had no discernable effect on localization or packaging. Using super resolution microscopy vesicles, showed governs fraction loaded CD63. Interestingly, CD9-dependent suppression was rescued by pharmacological blockade endocytosis. Together, our data support a model where contributes regulation secretion an endocytosis-dependent manner reprogram contents microdomains.
Язык: Английский
Процитировано
1
Nano Today, Год журнала: 2025, Номер 63, С. 102748 - 102748
Опубликована: Апрель 8, 2025
Язык: Английский
Процитировано
1
Frontiers in Bioengineering and Biotechnology, Год журнала: 2024, Номер 12
Опубликована: Авг. 5, 2024
Wound healing is a complicated obstacle, especially for chronic wounds. Mesenchymal stem cell-derived exosomes may be promising cell-free approach treating skin wound healing. Exosomes can accelerate by attenuating inflammation, promoting angiogenesis, cell proliferation, extracellular matrix production and remodeling. However, many issues, such as off-target effects high degradation of in sites need to addressed before applying into clinical therapy. Therefore, the bioengineering technology has been introduced modify with greater stability specific therapeutic property. To prolong function time local concentration bed, use biomaterials load emerges strategy. In this review, we summarize biogenesis characteristics exosomes, role healing, applications modified-exosomes The challenges prospects are also discussed.
Язык: Английский
Процитировано
3
Stem Cell Research & Therapy, Год журнала: 2025, Номер 16(1)
Опубликована: Янв. 23, 2025
Interest in prostate cancer as a research topic has gradually increased. As result, series of innovative treatment strategies have emerged with an in-depth understanding the disease. Owing to their unique biological characteristics, mesenchymal stromal cell exosomes (MSC-Exos) garnered significant attention for potential deliver targeted drugs and enable precise treatment. Herein, MSC-Exos drug-delivery systems is reviewed. This review provides comprehensive introduction advantages these systems, current trends progress, well analysis challenges future directions. Moreover, this lays solid foundation continued development application MSC-Exos.
Язык: Английский
Процитировано
0
Future Pharmacology, Год журнала: 2025, Номер 5(1), С. 11 - 11
Опубликована: Март 6, 2025
Exosomes can transport regulatory biomolecules and are mediators of cellular signaling among metabolic tissues through endocrine mechanisms. Understanding the pathways processes underlying exosome-mediated inter-tissue communication is critical for elucidating molecular pathophysiology diseases such as obesity, type 2 diabetes mellitus (T2DM), cardiovascular disorders. Consequently, these mechanisms represent novel promising targets pharmacological regulation. We examined current knowledge regarding exosome physiology, interaction with target tissues, its role in tissue communication. also analyzed secretory profiles exosomes emphasizing their roles adipose tissue, liver, pancreas, skeletal muscle, small intestine, while discussing association diseases. In this sense, we propose exosomal pentad a framework highlighting inter-organ communication, where may regulate axis involving tissues. This model aligns ominous octet but emphasizes key regulators homeostasis potential therapeutic targets. The treatment emerges area pharmacologic exploration. For instance, strategies that prevent binding or expression cargo molecules miRNAs could be designed, using antagomiRs nanoparticles. Additionally, integrins like αvβ5 on membrane blocked monoclonal antibodies engineered targeted delivery molecules. Exosomes, regulation, hold to design precise molecular-level therapies minimizing systemic side effects.
Язык: Английский
Процитировано
0
Nature Reviews Bioengineering, Год журнала: 2025, Номер unknown
Опубликована: Март 17, 2025
Язык: Английский
Процитировано
0