Transcriptomic and Histological Characterization of Telocytes in the Human Dorsal Root Ganglion

The Journal of Comparative Neurology, Год журнала: 2025, Номер 533(3)

Опубликована: Март 1, 2025

ABSTRACT Telocytes are interstitial cells characterized by long processes that span considerable distances within tissues, likely facilitating coordination and interaction with various cell types. Although present in central peripheral neuronal their role remains elusive. Dorsal root ganglia (DRG) house pseudounipolar afferent neurons responsible for transmitting signals related to temperature, proprioception, nociception. This study aimed investigate the presence function of telocytes human DRG examining transcriptional profile, anatomical location, ultrastructure. Combined expression CD34 PDGFRA is a marker gene set telocytes, our sequencing data revealed expressing comprise roughly 1.5%–3% cells. putative telocytes. Further analysis identified nine subclusters enriched cluster‐specific genes. Kyoto Encyclopedia Genes Genomes (KEGG) Gene Ontology (GO) pathway suggested vascular, immune, connective tissue‐associated telocyte subtypes, mapping over 3000 potential receptor–ligand interactions between sensory these were using ligand–receptors interactome platform. Immunohistochemistry CD34+ve endoneural space DRGs, next neuron–satellite complexes, perivascular spaces nerve fiber bundles, consistent analysis. Transmission electron microscopy (TEM) confirmed location identifying characteristic elongated nucleus, thin telopodes containing vesicles, often surrounded basal lamina. provides first complex tissue, specifically DRG, highlighting functional differences based on tissue while revealing no significant ultrastructural variations.

Язык: Английский

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Mitigating ambient RNA and doublets effects on single cell transcriptomics analysis in cancer research

Cancer Letters, Год журнала: 2025, Номер unknown, С. 217693 - 217693

Опубликована: Апрель 1, 2025

Язык: Английский

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Established and emerging roles of protein kinases in regulating primary sensory neurons in injury-and inflammation-associated pain

Expert Opinion on Therapeutic Targets, Год журнала: 2025, Номер unknown

Опубликована: Апрель 8, 2025

Recent seminal neuroscience research has significantly increased our knowledge on cellular and molecular responses of various cells in the pain pathway to peripheral nerve injuries inflammatory processes. Transcriptomic epigenetic analysis primary sensory neurons (PSNs) animal models revealed new insights into altered gene expression profiles modifications, which, via increasing spinal nociceptive input, lead development pain. Among classes molecules involved driving differential expression, protein kinases, enzymes that catalyze phosphorylation molecules, are emerging control histone modification chromatin remodeling needed for alteration transcriptional activity. Here, we focused how kinases contribute transcriptomic changes pathways induced reprogramming within PSNs upon injury inflammation. We conducted systematic literature search across multiple databases, including PubMed, NIH ClinicalTrials.gov portal GEOData from 1980-2024 compared kinase frequencies between publicly available RNA sequencing datasets investigated differences levels after injury. Novel findings support a concept constitute regulatory hubs PSNs, which offers novel analgesic approaches.

Язык: Английский

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Role of specialized sensory neuron subtypes in modulating peripheral immune responses

Immunity, Год журнала: 2025, Номер unknown

Опубликована: Май 1, 2025

Язык: Английский

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Nageotte nodules in human dorsal root ganglia reveal neurodegeneration in diabetic peripheral neuropathy

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Май 5, 2025

Язык: Английский

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