3D mechanical confinement directs muscle stem cell fate and function DOI Creative Commons

GaYoung Park,

Josh A. Grey,

Foteini Mourkioti

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 4, 2024

ABSTRACT Muscle stem cells (MuSCs) play a crucial role in skeletal muscle regeneration, residing niche that undergoes dimensional and mechanical changes throughout the regeneration process. This study investigates how three-dimensional (3D) confinement stiffness encountered by MuSCs during later stages of regulate their function, including stemness, activation, proliferation, differentiation. We engineered an asymmetric 3D hydrogel bilayer platform with tunable physical constraints to mimic regenerating MuSC niche. Our results demonstrate increased maintains Pax7 expression, reduces activation inhibits differentiation, is associated smaller nuclear size decreased H4K16ac levels, suggesting modulates both architecture epigenetic regulation. unconfined two-dimensional (2D) environments exhibited larger nuclei higher expression compared those more confined conditions, leading progressive expansion, myogenic commitment. highlights importance cues fate regulation, acting as brake on commitment, offering novel insights into mechano-epigenetic mechanisms govern behavior regeneration.

Язык: Английский

3D Mechanical Confinement Directs Muscle Stem Cell Fate and Function DOI Creative Commons

GaYoung Park,

Josh A. Grey,

Foteini Mourkioti

и другие.

Advanced Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 4, 2025

Muscle stem cells (MuSCs) play a crucial role in skeletal muscle regeneration, residing niche that undergoes dimensional and mechanical changes throughout the regeneration process. This study investigates how 3D confinement stiffness encountered by MuSCs during later stages of regulate their function, including stemness, activation, proliferation, differentiation. An asymmetric hydrogel bilayer platform is engineered with tunable physical constraints to mimic regenerating MuSC niche. These results demonstrate increased maintains Pax7 expression, reduces activation inhibits differentiation, associated smaller nuclear size decreased H4K16ac levels, suggesting modulates both architecture epigenetic regulation. unconfined 2D environments exhibit larger nuclei higher expression compared those more confined conditions, leading progressive expansion, myogenic commitment. highlights importance cues fate regulation, acting as brake on commitment, offering novel insights into mechano-epigenetic mechanisms govern behavior regeneration.

Язык: Английский

Процитировано

0
Viscoelastic High‐Molecular‐Weight Hyaluronic Acid Hydrogels Support Rapid Glioblastoma Cell Invasion with Leader–Follower Dynamics DOI Open Access
Emily M. Carvalho, Erika A. Ding,

Atul Saha

и другие.

Advanced Materials, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 7, 2024

Hyaluronic acid (HA), the primary component of brain extracellular matrix, is increasingly used to model neuropathological processes, including glioblastoma (GBM) tumor invasion. While elastic hydrogels based on crosslinked low-molecular-weight (LMW) HA are widely exploited for this purpose and have proven valuable discovery screening, tissue both viscoelastic rich in high-MW (HMW) HA, it remains unclear how these differences influence To address question, comprised either HMW (1.5 MDa) or LMW (60 kDa) introduced, characterized, applied GBM invasion studies. Unlike hydrogels, relax stresses quickly, a similar extent as tissue, greater than many conventional HA-based scaffolds. cells implanted within invade much more rapidly their counterparts exhibit distinct leader-follower dynamics. Leader adopt dendritic morphologies invasive observed vivo. Transcriptomic, pharmacologic, imaging studies suggest that leader exploit hyaluronidase, an enzyme strongly enriched human GBMs, prime path followers. This study offers new insight into properties drive argues use highly stress-relaxing materials GBM.

Язык: Английский

Процитировано

2
Carboxymethyl chitosan oligosaccharide enzymatic hydroxylates with reactive oxygen species scavenging and anti-inflammatory activity for topical treatment of skin photodamage DOI Creative Commons
Ziming Zhu, Hui Li,

Xiansen Lv

и другие.

Carbohydrate Polymer Technologies and Applications, Год журнала: 2024, Номер unknown, С. 100612 - 100612

Опубликована: Ноя. 1, 2024

Язык: Английский

Процитировано

1
3D mechanical confinement directs muscle stem cell fate and function DOI Creative Commons

GaYoung Park,

Josh A. Grey,

Foteini Mourkioti

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Окт. 4, 2024

ABSTRACT Muscle stem cells (MuSCs) play a crucial role in skeletal muscle regeneration, residing niche that undergoes dimensional and mechanical changes throughout the regeneration process. This study investigates how three-dimensional (3D) confinement stiffness encountered by MuSCs during later stages of regulate their function, including stemness, activation, proliferation, differentiation. We engineered an asymmetric 3D hydrogel bilayer platform with tunable physical constraints to mimic regenerating MuSC niche. Our results demonstrate increased maintains Pax7 expression, reduces activation inhibits differentiation, is associated smaller nuclear size decreased H4K16ac levels, suggesting modulates both architecture epigenetic regulation. unconfined two-dimensional (2D) environments exhibited larger nuclei higher expression compared those more confined conditions, leading progressive expansion, myogenic commitment. highlights importance cues fate regulation, acting as brake on commitment, offering novel insights into mechano-epigenetic mechanisms govern behavior regeneration.

Язык: Английский

Процитировано

0