Constant surface area-to-volume ratio during cell growth as a design principle in mammalian cells DOI Creative Commons
Weida Wu, Alice Lam,

Kayla Suarez

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 4, 2024

ABSTRACT All cells are subject to geometric constraints, such as surface area-to-volume (SA/V) ratio, that impact cell functions and force biological adaptations. Like the SA/V ratio of a sphere, it is generally assumed decreases size increases. Here, we investigate this in near-spherical mammalian using single-cell measurements mass proteins, well imaging plasma membrane morphology. We find remains surprisingly constant grow larger. This observation largely independent cycle amount growth. Consequently, growth results increased folding, which simplifies cellular design by ensuring sufficient area for division, nutrient uptake deformation at all sizes.

Язык: Английский

Plasma membrane folding enables constant surface area-to-volume ratio in growing mammalian cells DOI
Weida Wu, Alice Lam,

Kayla Suarez

и другие.

Current Biology, Год журнала: 2025, Номер unknown

Опубликована: Март 1, 2025

Язык: Английский

Процитировано

0
Advanced microfluidic systems with temperature modulation for biological applications DOI
Juhee Ko, Jungchul Lee

Biomicrofluidics, Год журнала: 2025, Номер 19(3)

Опубликована: Май 1, 2025

Recent advances in microfluidic technology have shown the importance of precise temperature control a wide range biological applications. This perspective review presents comprehensive overview state-of-the-art platforms that utilize thermal modulation for various applications, such as rapid nucleic acid amplification, targeted hyperthermia cancer therapy, and efficient cellular lysis. We detail heating mechanisms-including nanoparticle-driven induction, photothermal conversion, electrothermal approaches (both external on-chip)-and discuss how they are integrated within lab-on-a-chip systems. In parallel, advanced multi-modal sensing methods microfluidics, ranging from conventional sensors to cutting-edge quantum-based techniques using nanodiamond nitrogen-vacancy centers suspended microchannel resonators, highlighted. By integrating capabilities into these platforms, broader applications enabled, including single-cell analysis, metabolic profiling, scalable diagnostics. Looking ahead, overcoming challenges system integration, scalability, cost-effectiveness will be essential harnessing their full potential. Future developments this field expected drive evolution technologies, ultimately enabling breakthroughs precision medicine high-throughput biomedical

Язык: Английский

Процитировано

0
Cyanobacteria newly isolated from marine volcanic seeps display rapid sinking and robust, high-density growth DOI Creative Commons
Max G. Schubert, Tzu‐Chieh Tang, Isabella M. Goodchild-Michelman

и другие.

Applied and Environmental Microbiology, Год журнала: 2024, Номер 90(11)

Опубликована: Окт. 29, 2024

ABSTRACT Cyanobacteria are photosynthetic organisms that play important roles in carbon cycling and promising bioproduction chassis. Here, we isolate two novel cyanobacteria with 4.6Mbp genomes, UTEX 3221 3222, from a unique marine environment naturally elevated CO₂. We describe complete genome sequences for both isolates and, focusing on 3222 due to its planktonic growth liquid, characterize biotechnologically relevant biomass characteristics. outpaces other fast-growing model strains solid medium. It can double every 2.35 hours liquid medium grows high density (>31 g/L dry weight) batch culture, nearly of Synechococcus sp. PCC 11901, whose high-density was recently reported. In addition, sinks readily, settling more quickly than strains, suggesting favorable economics harvesting biomass. These traits may make compelling choice dioxide removal (CDR) Overall, find bio-prospecting environments CO₂ uncover CO₂-metabolizing IMPORTANCE provide potential avenue biomanufacturing combatting climate change via high-efficiency sequestration. This study identifies isolated geochemical describes their behavior biochemical composition. appear tractable research model, cultures made publicly available alongside information about culture maintenance. Application these sequestration and/or is discussed, including unusual, rapid characteristics the scaled culture.

Язык: Английский

Процитировано

1
Constant surface area-to-volume ratio during cell growth as a design principle in mammalian cells DOI Creative Commons
Weida Wu, Alice Lam,

Kayla Suarez

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 4, 2024

ABSTRACT All cells are subject to geometric constraints, such as surface area-to-volume (SA/V) ratio, that impact cell functions and force biological adaptations. Like the SA/V ratio of a sphere, it is generally assumed decreases size increases. Here, we investigate this in near-spherical mammalian using single-cell measurements mass proteins, well imaging plasma membrane morphology. We find remains surprisingly constant grow larger. This observation largely independent cycle amount growth. Consequently, growth results increased folding, which simplifies cellular design by ensuring sufficient area for division, nutrient uptake deformation at all sizes.

Язык: Английский

Процитировано

0