Investigation of the development and evolution of the mammalian cerebrum using gyrencephalic ferrets DOI Creative Commons

Masanori Imamura,

Mayuko Yoshino,

Hiroshi Kawasaki

и другие.

European Journal of Cell Biology, Год журнала: 2024, Номер 103(4), С. 151466 - 151466

Опубликована: Ноя. 10, 2024

Mammalian brains have evolved a neocortex, which has diverged in size and morphology different species over the course of evolution. In some mammals, substantial increase number neurons glial cells resulted expansion folding cerebrum, it is believed that these evolutionary changes contributed to acquisition higher cognitive abilities mammals. However, their underlying molecular cellular mechanisms remain insufficiently elucidated. A major difficulty addressing stemmed from lack appropriate animal models, as conventional experimental animals such mice rats small without structurally obvious folds. Therefore, researchers including us focused on using ferrets instead rats. Ferrets are domesticated carnivorous mammals with gyrencephalic and, notably, they amenable genetic manipulations utero electroporation knock out genes cerebrum. this review, we highlight recent research into development evolution cortical folds ferrets.

Язык: Английский

Gene regulatory landscape of cerebral cortex folding DOI Creative Commons
Aditi Singh, Lucia Del-Valle-Anton, Camino de Juan Romero

и другие.

Science Advances, Год журнала: 2024, Номер 10(23)

Опубликована: Июнь 5, 2024

Folding of the cerebral cortex is a key aspect mammalian brain development and evolution, defects are linked to severe neurological disorders. Primary folding occurs in highly stereotyped patterns that predefined cortical germinal zones by transcriptomic protomap. The gene regulatory landscape governing emergence this protomap remains unknown. We characterized spatiotemporal dynamics expression active epigenetic (H3K27ac) across prospective folds fissures ferret. Our results show begins emerge at early embryonic stages, it involves cell-fate signaling pathways. H3K27ac reveals developmental restriction engages known regulators, including transcription factor Cux2 . Manipulating progenitors changed their proliferation pattern ferret, caused selective transcriptional changes as revealed single-cell RNA sequencing analyses. findings highlight relevance mechanisms defining folding.

Язык: Английский

Процитировано

5
Indirect neurogenesis in space and time DOI
Stefan Thor

Nature reviews. Neuroscience, Год журнала: 2024, Номер 25(8), С. 519 - 534

Опубликована: Июль 1, 2024

Язык: Английский

Процитировано

5
Two independent translocation modes drive neural stem cell dissemination into the human fetal cortex DOI Creative Commons
Ryszard Wimmer,

Pauline Lestienne,

Christοphe Brunet

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 9, 2025

Abstract The strong size increase of the human neocortex is supported both by amplification and basal translocation a neural stem cell population, radial glial cells (or bRG cells). Using live imaging second trimester fetal tissue cortical organoids, we identify two independent modes for colonization neocortex. On top an actomyosin-dependent movement called mitotic somal (MST), microtubule-dependent motion occurring during interphase, that call interphasic (IST). We show IST driven LINC complex, through nuclear envelope recruitment dynein motor its activator LIS1. Consequently, severely altered in LIS1 patient-derived organoids. also demonstrate MST occurs prometaphase spindle event. controlled rounding molecular pathway, via Moesin Vimentin, driving translocation. report 85% due to IST, total 0,67 mm per month gestation. Our work identifies how colonize cortex, further shows are conserved bRG-related migrating glioblastoma cells.

Язык: Английский

Процитировано

0
Electroporation Techniques to Target Subependymal Zone Neurogenic Niche DOI
Andrzej Cwetsch, Cristina Gil‐Sanz

Methods in molecular biology, Год журнала: 2025, Номер unknown, С. 171 - 182

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0
Distinct mitotic dynamics and neuronal migration patterns between gyri and sulci in the ferret neocortex during cortical folding DOI
Youning Lin, Shi‐Yuan Tong, Jun-Wei Cao

и другие.

Neuroscience, Год журнала: 2025, Номер unknown

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
How radial glia progenitor lineages generate cell-type diversity in the developing cerebral cortex DOI Creative Commons
Fabrizia Pipicelli, Ana Villalba, Simon Hippenmeyer

и другие.

Current Opinion in Neurobiology, Год журнала: 2025, Номер 93, С. 103046 - 103046

Опубликована: Май 17, 2025

The cerebral cortex is arguably the most complex organ in humans. cortical architecture characterized by a remarkable diversity of neuronal and glial cell types that make up its circuits. Following precise temporally ordered program, radial glia progenitor (RGP) cells generate all excitatory projection neurons cell-types. Cortical are produced either directly or via intermediate progenitors, through indirect neurogenesis. How extensive cell-type generated during development remains, however, fundamental open question. do RGPs quantitatively qualitatively neocortical neurons? does direct neurogenesis contribute to establishment lineage heterogeneity? Whether represent homogeneous and/or multipotent population, if consist heterogeneous groups currently also not known. In this review, we will summarize latest findings contributed deeper insight into above key questions.

Язык: Английский

Процитировано

0
Conservation, alteration, and redistribution of mammalian striatal interneurons DOI Creative Commons
Emily K. Corrigan, Michael DeBerardine, Aunoy Poddar

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Июль 30, 2024

Mammalian brains vary in size, structure, and function, but the extent to which evolutionarily novel cell types contribute this variation remains unresolved. Recent studies suggest there is a primate-specific population of striatal inhibitory interneurons, TAC3 interneurons5. However, has not yet been detailed analysis spatial phylogenetic distribution population. Here, we profile single gene expression developing pig (an ungulate) ferret (a carnivore), representing 94 million years divergence from primates, assign newborn neurons initial classes first specified during development. We find that class interneurons represents an ancestral also deployed towards cortex ferret. In adult mouse, uncover rare expressing Tac2, ortholog TAC3, ventromedial striatum, prompting reexamination mouse interneuron by targeted enrichment their precursors. conclude conserved across Boreoeutherian mammals, with Th subset expresses Tac2. This study suggests telencephalic are largely evolution, neuronal mammalian brain change through redistribution fate refinement, rather than derivation precursors early

Язык: Английский

Процитировано

3
Kinesin-like motor protein KIF23 maintains neural stem and progenitor cell pools in the developing cortex DOI Creative Commons

Sharmin Naher,

Kenji Iemura, Satoshi Miyashita

и другие.

The EMBO Journal, Год журнала: 2024, Номер 44(2), С. 331 - 355

Опубликована: Дек. 4, 2024

Язык: Английский

Процитировано

2
Entosis implicates a new role for P53 in microcephaly pathogenesis, beyond apoptosis DOI
Noelle A. Sterling, Seo‐Hee Cho, Seonhee Kim

и другие.

BioEssays, Год журнала: 2024, Номер 46(8)

Опубликована: Май 22, 2024

Abstract Entosis, a form of cell cannibalism, is newly discovered pathogenic mechanism leading to the development small brains, termed microcephaly, in which P53 activation was found play major role. Microcephaly with entosis, Pals1 mutant mice, displays that promotes entosis and apoptotic death. This previously unappreciated represents novel cellular dynamic dividing cortical progenitors responsible for loss. To date, various recent models microcephaly have bolstered importance death microcephaly. caused by mitotic delay or DNA damage manifests can be suppressed removal these animal models. Such genetic studies attest as quality control meant eliminate genomically unfit cells minimal involvement actual function associated genes. In this review, we summarize known role variety introduce wherein entotic cannibalism neural triggered activation.

Язык: Английский

Процитировано

1
Microdissection and Single-Cell Suspension of Neocortical Layers From Ferret Brain for Single-Cell Assays DOI Creative Commons

Lucia Del-Valle-Anton,

Salma Amin, Vı́ctor Borrell

и другие.

BIO-PROTOCOL, Год журнала: 2024, Номер 14(1361)

Опубликована: Янв. 1, 2024

Brain development is highly complex and dynamic. During this process, the different brain structures acquire new components, such as cerebral cortex, which builds up germinal cortical layers during its development. The genetic study of structure has been commonly approached by bulk-sequencing entire cortex a whole. Here, we describe methodology to layered tissue in all complexity microdissecting two at developmental time points. This protocol combined with step-by-step explanation dissociation that provides high-quality cells ready be analyzed newly developed single-cell assays, scRNA-seq, scATAC-seq, TrackerSeq. Altogether, approach increases resolution analyses from compared bulk studies. It also facilitates laboratory animal models recapitulate human better than mice, like ferrets. Key features • Microdissection individual developing living slices. Enzymatic mechanical generates suspensions available for high-throughput assays. Protocol optimized embryonic early postnatal ferret cortex.

Язык: Английский

Процитировано

0