Understanding the molecular diversity of synapses
Nature reviews. Neuroscience,
Год журнала:
2024,
Номер
unknown
Опубликована: Дек. 5, 2024
Язык: Английский
Synaptic Targets and Cellular Sources of CB1 Cannabinoid Receptor and Vesicular Glutamate Transporter‐3 Expressing Nerve Terminals in Relation to GABAergic Neurons in the Human Cerebral Cortex
European Journal of Neuroscience,
Год журнала:
2025,
Номер
61(1)
Опубликована: Янв. 1, 2025
ABSTRACT
Cannabinoid
receptor
1
(CB1)
regulates
synaptic
transmission
through
presynaptic
receptors
in
nerve
terminals,
and
its
physiological
roles
are
of
clinical
relevance.
The
cellular
sources
targets
CB1‐expressing
terminals
the
human
cerebral
cortex
undefined.
We
demonstrate
a
variable
laminar
pattern
CB1‐immunoreactive
axons
electron
microscopically
show
that
CB1‐positive
GABAergic
make
type‐2
synapses
innervating
dendritic
shafts
(69%),
spines
(20%)
somata
(11%)
neocortical
layers
2–3.
Of
CB1‐immunopositive
25%
were
vesicular‐glutamate‐transporter‐3
(VGLUT3)‐immunoreactive,
suggesting
GABAergic/glutamatergic
co‐transmission
on
shafts.
In
vitro
recorded
labelled
VGLUT3
or
interneurons
expressed
cholecystokinin,
vasoactive‐intestinal‐polypeptide
calretinin,
had
diverse
firing,
dendrites,
included
rosehip,
neurogliaform
basket
cells,
but
not
double
bouquet
axo‐axonic
cells.
innervated
pyramidal
cells
interneurons.
Glutamatergic
formed
type‐1
some
positive
for
CB1
with
distribution
appeared
different
from
terminals.
From
sampled
VGLUT3‐positive
60%
(80%)
52%
also
VGLUT1,
intracortical
origin.
Some
immunopositive
vesicular‐monoamine‐transporter‐2,
5‐HT/glutamate
co‐transmission.
Overall,
results
GABA
release
mainly
to
both
predict
CB1‐regulated
co‐release
glutamate
single
cortical
co‐existence
multiple
vesicular
transporters
select
population
probably
originating
neurons
cortex.
Язык: Английский
Conditional Deletion of CB1 Receptor in Parvalbumin‐Expressing GABAergic Neurons Results in Hearing Loss and Abnormal Auditory Brainstem Response in Mice
European Journal of Neuroscience,
Год журнала:
2025,
Номер
61(6)
Опубликована: Март 1, 2025
ABSTRACT
Cannabinoid
receptors
1
(CB1Rs)
are
widely
expressed
throughout
the
central
auditory
system
and
play
important
roles
in
synaptic
plasticity,
function,
development.
However,
specific
functions
of
CB1Rs
distinct
neuronal
subtypes
remain
largely
unclear.
In
current
study,
we
investigated
whether
CB1R
deficiency
parvalbumin
(PV)‐expressing
interneurons,
a
major
class
GABAergic
affects
hearing
function.
We
first
systematically
examined
localization
distribution
mouse
using
double‐label
immunofluorescence
confocal
laser
scanning
microscopy.
Our
findings
revealed
that
distributed
across
various
nuclei,
with
especially
high
expression
levels
observed
cochlear
nucleus
(CN),
superior
olivary
complex
(SOC),
lateral
lemniscus
(LL).
Furthermore,
established
genetic
model
for
conditional
knockout
specifically
PV
performed
brainstem
response
(ABR)
tests
to
measure
effects
this
deletion
on
hearing.
Surprisingly,
analysis
ABR
from
interneurons
elevated
threshold
at
32
kHz,
prolongated
latency
Wave
1,
decreased
amplitudes
Waves
1–4.
provide
solid
anatomical
foundation
further
investigations
into
functional
highlight
critical
role
inhibitory
maintaining
normal
Язык: Английский
Integrating endocannabinoid signaling, CCK interneurons, and hippocampal circuit dynamics in behaving animals
Neuron,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 1, 2025
Язык: Английский
Cannabinoid CB1 receptor-sensitive neurodevelopmental processes and trajectories
Molecular Psychiatry,
Год журнала:
2025,
Номер
unknown
Опубликована: Май 19, 2025
Abstract
As
high-potency
cannabis
(with
high
Δ
9
-Tetrahydrocannabinol
content)
becomes
easily
accessible
and
widespread,
it
is
of
extreme
importance
for
public
health
that
a
scientific
platform
used
to
implement
practical
guidelines,
particularly
at-risk
populations.
Many
reviews
have
been
written
in
the
past
decade
summarizing
impact
developing
brain.
One
critical
concept
frequently
mentioned
but
not
discussed
detail
whether
there
are
sensitive
neurodevelopmental
events
driving
age-specific
sensitivity
cannabis,
those
mediated
by
cannabinoid
type
1
receptor
signaling.
By
integrating
available
data
from
humans
animal
models,
goal
present
expert
review
article
provide
mechanistic
overview
on
how
exposure
during
periods
neural
circuit
plasticity
development
can
result
lasting
consequences.
Here
we
frontal
cortex
as
proxy
align
trajectory
brain
system
between
rodents.
Both
strengths
limitations
studies
effects
cannabinoids
were
using
developmental
framework
which
adaptations
considered.
Such
an
approach
needed
key
variables
through
lifespan,
turn
will
valuable
insights
applicable
human
defining
underpinning
mechanisms
changes
childhood
adolescence,
thereafter
young
adulthood.
Язык: Английский
Simulated complexes formed from a set of postsynaptic proteins suggest a localised effect of a hypomorphic Shank mutation
BMC Neuroscience,
Год журнала:
2024,
Номер
25(1)
Опубликована: Июль 6, 2024
The
postsynaptic
density
is
an
elaborate
protein
network
beneath
the
membrane
involved
in
molecular
processes
underlying
learning
and
memory.
built
up
from
same
major
proteins
but
its
exact
composition
organization
differs
between
synapses.
Mutations
perturbing
protein:
interactions
generally
occurring
this
might
lead
to
effects
specific
for
cell
types
or
processes,
understanding
of
which
can
be
especially
challenging.
Язык: Английский
Functional Adaptation in the Brain Habenulo–Mesencephalic Pathway During Cannabinoid Withdrawal
Cells,
Год журнала:
2024,
Номер
13(21), С. 1809 - 1809
Опубликована: Ноя. 1, 2024
The
mesolimbic
reward
system
originating
from
dopamine
neurons
in
the
ventral
tegmental
area
(VTA)
of
midbrain
shows
a
profound
reduction
function
during
cannabinoid
withdrawal.
This
condition
may
underlie
aversive
states
that
lead
to
compulsive
drug
seeking
and
relapse.
lateral
habenula
(LHb)
exerts
negative
control
over
VTA
via
GABA
rostromedial
nucleus
(RMTg),
representing
potential
convergence
point
for
drug-induced
opponent
processes.
We
hypothesized
LHb-RMTg
pathway
might
be
causally
involved
hypodopaminergic
state
To
induce
Δ
Язык: Английский
Synaptic targets and cellular sources of CB1 cannabinoid receptor and vesicular glutamate transporter-3 expressing nerve terminals in relation to GABAergic neurons in the human cerebral cortex
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 16, 2024
Abstract
Cannabinoid
receptor
1
(CB1)
regulates
synaptic
transmission
through
presynaptic
receptors
in
nerve
terminals,
and
its
physiological
roles
are
of
clinical
relevance.
The
cellular
sources
targets
CB1-expressing
terminals
the
human
cerebral
cortex
undefined.
We
demonstrate
a
variable
laminar
pattern
CB1-immunorective
axons
electron
microscopically
show
that
CB1-positive
GABAergic
make
type-2
synapses
innervating
dendritic
shafts
(69%),
spines
(20%)
somata
(11%)
neocortical
layers
2-3.
Of
CB1-immunopositive
25%
were
vesicular-glutamate-transporter-3
(VGLUT3)-immunoreactive,
suggesting
GABAergic/glutamatergic
co-transmission
on
shafts.
In
vitro
recorded
labelled
VGLUT3
or
interneurons
expressed
cholecystokinin,
vasoactive-intestinal-polypeptide
calretinin,
had
diverse
firing,
dendrites,
included
rosehip,
neurogliaform
basket
cells,
but
not
double
bouquet
axo-axonic
cells.
innervated
pyramidal
cells
interneurons.
Most
glutamatergic
formed
type-1
some
positive
for
CB1
concentrated
active
zone,
unlike
terminals.
From
sampled
VGLUT3-positive
60%
with
(80%)
52%
also
VGLUT1,
intracortical
origin.
Some
immunopositive
vesicular-monoamine-transporter-2,
5-HT/glutamate
co-transmission.
Overall,
results
GABA
release
mainly
to
both
interneurons,
predict
CB1-regulated
co-release
glutamate
from
single
cortical
co-existence
multiple
vesicular
transporters
select
population
probably
originating
neurons
cortex.
Язык: Английский