Deciphering distinct spatial alterations in N-glycan expression profiles in the spinal cord and brain of male rats in a neuropathic pain model
Cellular & Molecular Biology Letters,
Год журнала:
2025,
Номер
30(1)
Опубликована: Март 11, 2025
Abstract
Background
Neuropathic
pain
is
a
complex
condition
resulting
from
damage
or
disease
in
the
somatosensory
nervous
system,
causing
significant
physical
and
emotional
distress.
Despite
its
profound
impact,
underlying
causes
treatment
methods
of
neuropathic
remain
poorly
understood.
Methods
To
better
understand
this
condition,
we
conducted
first
study
examining
spatial
distribution
dynamic
expression
changes
N-glycan
molecules
that
play
crucial
role
system
function
sustainable
signal
transmission
across
multiple
regions
spinal
cord
brain
an
experimentally
induced
model,
using
matrix-assisted
laser
desorption/ionization
mass
spectrometry
imaging
(MALDI
MSI).
Results
Our
findings
revealed
induces
various
brain.
Notably,
discovered
distinct
glycan
profiles
between
brain,
with
N-glycans
downregulated
upregulated
at
time
when
mechanical
allodynia
sustained
following
nerve
ligation
(SNL).
Significant
were
observed
dorsal
laminae
IV/V/VI
ventral
horn
cord.
Additionally,
marked
detected
contralateral
primary
sensory
cortex
(S1)
hindlimb
area
(S1HL).
Furthermore,
upregulation
thalamus,
anterior
cingulate
(ACC),
medial
prefrontal
(mPFC)
both
ipsilateral
Conclusions
Given
are
implicated
processing
yet
their
precise
remains
unclear,
our
highlights
need
to
explore
N-glycosylation
more
nuanced
focus
on
This
research
provides
new
insights
into
mechanisms
persistent
lays
groundwork
for
future
studies
development
targeted
therapeutic
strategies.
Graphical
Язык: Английский
Domain-general neural effects of associative learning and expectations on pain and hedonic taste perception
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 16, 2025
Abstract
Predictive
cues
significantly
influence
perception
through
associative
learning.
However,
it
is
unknown
whether
circuits
are
conserved
across
domains.
We
investigated
how
learning
influences
perceived
intensity
and
valence
of
pain
hedonic
taste
the
mechanisms
that
support
expectancy-based
modulation
vary
as
a
function
aversiveness
modality.
Sixty
participants
were
randomly
assigned
to
receive
either
painful
heat,
unpleasant
liquid
saline,
or
pleasant
sucrose
during
fMRI
scanning.
Following
conditioning,
initially
associated
with
low
high
outcomes
intermittently
followed
by
stimuli
calibrated
elicit
medium
ratings.
Learned
modulated
expectations
subjective
similarly
Consistent
this,
orbitofrontal
cortex
exhibited
domain-general
anticipatory
activation.
The
left
anterior
insula
mediated
cue
effects
on
intensity,
whereas
thalamus
valence.
Notably,
these
two
regions
nearly
identical
those
previously
implicated
in
mediating
(nearly
(Atlas
et
al.,
2010).
Pain
specificity
was
evident
when
we
measured
variations
stimulus
used
univariate
multivariate
approaches,
but
there
minimal
evidence
modality
examined
trials.
These
findings
suggest
shared
neural
mediate
learned
perception,
linking
other
areas
affective
processing
Язык: Английский