Machine Learning Assisted Nanofluidic Array for Multiprotein Detection
ACS Nano,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 26, 2025
Solid-state
nanopore
and
nanochannel
biosensors
have
revolutionized
protein
detection
by
offering
label-free,
highly
sensitive
analyses.
Traditional
sensing
systems
(1st
2nd
stages)
primarily
focus
on
inner
wall
(IW)
interactions,
facing
challenges
such
as
complex
preparation
processes,
variable
entry
angles,
conformational
changes,
leading
to
irregular
events.
To
address
these
limitations,
recent
advancements
(3rd
stage)
shifted
toward
outer
surface
(OS)
functionalization
but
are
constrained
single-protein
recognition
models.
Herein,
we
show
a
machine
learning
assisted
nanofluidic
array
(MANY)
system
(4th
that
integrates
supervised
dimensionality
reduction
strategy
with
photoresponsive
MoS2
functionalized
nonspecific
functional
elements
(FEarray)
at
the
OS.
This
approach
serves
proof-of-concept
for
probe-free
of
multiple
proteins
100%
accuracy,
highlighting
its
significant
potential
rapid
diagnostics
in
future
disease
applications.
Язык: Английский
Detection of Analytes with the Outer Surface of Solid-State Nanochannels: From pm to μm
Accounts of Chemical Research,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 7, 2025
ConspectusAccurately
simulating
or
sensitively
monitoring
specific
substances,
such
as
ions,
molecules,
and
proteins
in
the
life
process,
is
essential
for
gaining
a
fundamental
comprehension
of
underlying
biological
mechanism,
which
has
been
trending
topic
many
years.
Solid-state
nanochannels,
inspired
by
ion
channels,
have
developed
decades
achieved
significant
success,
representing
forefront
interdisciplinary
fields
bioanalytical
chemistry
nanotechnology.
Typically,
solid-state
nanochannels
with
pore
size
less
than
100
nm
are
selected
to
construct
nanochannel-based
biosensors,
can
be
an
excellent
platform
analyze
small
analytes,
ions
restricted
space
simulate
intricate
process
transport
living
organisms.
Furthermore,
integrating
functional
components
that
termed
probes
into
artificial
devices,
nanochannel
system
emerged
remarkable
tool
label-free
highly
sensitive
detection
practical
applications.
Nonetheless,
large
substances
(more
nanoscale
size)
consistently
posed
challenge,
since
previous
research
on
mainly
concentrated
contribution
at
inner
wall,
requires
biotargets
enter
successful
detection.
Moreover,
lack
testing
techniques
chemical
physical
properties
anchored
deep
inside
confined
results
unclear
working
another
issue
cannot
ignored.
The
requirement
more
efficient
extensive
spurred
in-depth
study
provides
innovative
insight
concentrating
outer
surface
(OS)
OS
(POS).In
this
Account,
several
approaches
constructing
modifying
POS
briefly
summarized.
Subsequently,
ultrasensitive
analytes
across
range
sizes,
encompassing
not
only
molecules
from
∼100
pm
∼2
but
also
∼20
μm
through
use
last
five
years,
introduced.
Through
characterization
precise
control
POS,
sensing
including
charge
wettability,
discussed
unambiguously.
Additionally,
intelligent
model
using
dual-signal
responses
electrical
optical
enhance
responsiveness
accuracy
quantitative
analysis
discussed,
distinguish
conformation
analyte
exposed
single
cysteine
thiol
group.
We
expect
timely
Account
will
offer
instructive
insights
development
facilitate
biomolecules
varying
sizes.
Язык: Английский
Construction of Biomimetic Nanochannel, Property Regulation, and Biomarker Detection
Small,
Год журнала:
2025,
Номер
unknown
Опубликована: Апрель 28, 2025
Abstract
The
significance
of
biomimetic
nanochannel
in
the
field
biosensors
is
gaining
increasing
recognition.
controllable
construction
nanochannels
and
their
performance
modulation
have
demonstrated
great
importance
obtained
wide
interest.
offer
high
sensitivity,
enabling
sensors
to
swiftly
identify
target
biomarkers
complex
biological
samples,
with
detection
limits
reaching
picomolar
level.
Furthermore,
they
demonstrate
exceptional
selectivity
reproducibility,
making
them
ideal
tools
for
biomarker
detection.
In
recent
years,
utilizing
shown
remarkable
detecting
a
range
biomarkers.
This
review
aims
explore
opportunities
challenges
associated
technology
biosensor
applications,
focusing
on
these
nanochannels,
as
well
applications
nucleic
acids,
proteins,
organisms,
small
molecules.
Providing
forward‐looking
insights
into
this
cutting‐edge
aspired,
particular
emphasis
technological
advancements,
addressing
current
challenges,
discussing
future
trends.
Язык: Английский