Advanced vibrational microscopes for life science
Nature Methods,
Год журнала:
2025,
Номер
22(5), С. 912 - 927
Опубликована: Май 1, 2025
Язык: Английский
A 20-year journey on the invention of vibrational photothermal microscopy
Nature Methods,
Год журнала:
2025,
Номер
22(5), С. 883 - 885
Опубликована: Май 1, 2025
Язык: Английский
Bioorthogonal Raman and IR probes for live cell metabolomics: a library
Sensors and Actuators B Chemical,
Год журнала:
2025,
Номер
unknown, С. 137363 - 137363
Опубликована: Янв. 1, 2025
Язык: Английский
Background-Free Mid-Infrared Photothermal Microscopy via Single-Shot Measurement of Thermal Decay
Analytical Chemistry,
Год журнала:
2025,
Номер
unknown
Опубликована: Фев. 18, 2025
Mid-infrared
photothermal
(MIP)
microscopy
is
an
emerging
tool
for
biological
imaging,
offering
high
sensitivity,
subcellular
resolution,
and
rapid
image
acquisition.
However,
the
MIP
signal
of
low
concentration
molecules
in
systems
often
hindered
or
masked
by
background
absorption,
largely
contributed
water,
resulting
from
H–O–H
scissors-bending
band
fingerprint
window
bend-libration
combination
cell-silent
window.
To
preserve
all
desired
signals
while
suppressing
background,
we
report
a
single-shot
time-resolved
measurement
that
allows
differentiation
between
analyte
based
on
their
distinct
dynamics.
The
results
show
thermal
decay
significantly
longer
than
intracellular
signal,
mainly
due
to
larger
mass
heat
capacity
water
compared
those
features.
Through
two-component
exponential
fitting,
successfully
differentiated
suppressed
preserving
both
windows.
By
leveraging
dynamics
differences
obtained
measurement,
effectively
remove
enhance
detection
small
system.
Язык: Английский
Ultrasensitive in vivo infrared spectroscopic imaging via oblique photothermal microscopy
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Окт. 3, 2024
Abstract
In
vivo
IR
spectroscopy
faces
challenges
due
to
poor
sensitivity
in
reflection
mode
and
low
resolution
at
micrometer
scale.
To
break
this
barrier,
we
report
an
oblique
photothermal
microscope
(OPTM)
enable
ultrasensitive
spectroscopic
imaging
of
live
subjects
sub-micron
resolution.
Classic
measurement
captures
only
a
small
fraction
probe
photons
through
pinhole
extract
the
signal.
Instead,
OPTM
uses
differential
split
detector
placed
on
sample
surface
collect
500-fold
more
suppress
laser
noise
by
12
fold
via
balanced
detection.
Leveraging
its
improved
sensitivity,
enables
low-dose
skin
without
photodamage.
Depth-resolved
metabolic
markers
beneath
mouse
human
is
shown.
Furthermore,
demonstrate
tracking
topical
drug
contents
within
skin.
Collectively,
presents
highly
sensitive
platform
for
situ
molecular
analysis.
Язык: Английский