Tea Aphid‐Induced β‐Elemene Biosynthesis by CsELE Enhances JA‐Dependent Herbivore Resistance in Tea Plants DOI

Lanxin Luo,

Ting Gao,

Yanni Deng

и другие.

Plant Cell & Environment, Год журнала: 2025, Номер unknown

Опубликована: Май 19, 2025

ABSTRACT Herbivore‐induced plant volatiles play a crucial role in enhancing resistance and adaptability. However, the key triggering this response their activation mechanisms remain largely unknown. Although tea aphids ( Toxoptera aurantii ) are common plantation pests, underlying biochemical pathways by which aphid‐induced Camellia sinensis contribute to aphid not well understood. In study, we measured jasmonic acid (JA) accumulation induced feeding using LC‐MS DESI‐IMS analysis. We also identified diverse array of through GC‐MS. Notably, stimulated β‐Elemene accumulation, ‘Shuchazao’ aphids. evaluated insecticidal activity contact toxicity repellent bioassays. For first time, CsELE as terpene synthase enzyme catalyzing final step biosynthesis from farnesyl pyrophosphate. Transient overexpression gene silencing affected thereby influencing Furthermore, Dual‐Luciferase Y1H assays revealed that MYC2a, core transcription factor JA signaling pathway, positively regulates expression. Overall, study advances our understanding transcriptional regulation pest attacks, providing new insights into ecological significance under biotic stress.

Язык: Английский

Natural variation of CsUGT71A60 determines growth and cold tolerance via regulating cytokinin glycosylation in Camellia sinensis DOI Creative Commons

Mingyue Zhao,

Jingming Wang, Xinyuan Hao

и другие.

Plant Biotechnology Journal, Год журнала: 2025, Номер unknown

Опубликована: Апрель 29, 2025

Summary Cold stress severely limits tea plant ( Camellia sinensis ) productivity, yet the molecular mechanisms underlying cold adaptation remain elusive. Here, we identified a cold‐inducible glycosyltransferase, CsUGT71A60, through integrative genome‐wide association studies (GWAS) and proteomic profiling. Natural variation in CsUGT71A60 was strongly associated with tolerance, as evidenced by linkage disequilibrium analysis of flanking SNPs. Functional characterization revealed that specifically catalyses glycosylation cis‐zeatin to form 9‐O‐glucoside vitro vivo. Overexpression Arabidopsis enhanced tolerance agronomic traits, including germination rate, tiller number seed weight, while delaying flowering. Transient silencing plants disrupted homoeostasis, impairing antioxidant defences osmotic regulation under stress. Mechanistically, transcription factor ARR (TEA021099) directly binds CRM elements promoter, activating its expression fine‐tune cytokinin signalling. This study unveils dual‐function glycosyltransferase orchestrates developmental plasticity, offering strategic target for breeding climate‐tolerance crops without yield penalties.

Язык: Английский

Процитировано

0
Tea Aphid‐Induced β‐Elemene Biosynthesis by CsELE Enhances JA‐Dependent Herbivore Resistance in Tea Plants DOI

Lanxin Luo,

Ting Gao,

Yanni Deng

и другие.

Plant Cell & Environment, Год журнала: 2025, Номер unknown

Опубликована: Май 19, 2025

ABSTRACT Herbivore‐induced plant volatiles play a crucial role in enhancing resistance and adaptability. However, the key triggering this response their activation mechanisms remain largely unknown. Although tea aphids ( Toxoptera aurantii ) are common plantation pests, underlying biochemical pathways by which aphid‐induced Camellia sinensis contribute to aphid not well understood. In study, we measured jasmonic acid (JA) accumulation induced feeding using LC‐MS DESI‐IMS analysis. We also identified diverse array of through GC‐MS. Notably, stimulated β‐Elemene accumulation, ‘Shuchazao’ aphids. evaluated insecticidal activity contact toxicity repellent bioassays. For first time, CsELE as terpene synthase enzyme catalyzing final step biosynthesis from farnesyl pyrophosphate. Transient overexpression gene silencing affected thereby influencing Furthermore, Dual‐Luciferase Y1H assays revealed that MYC2a, core transcription factor JA signaling pathway, positively regulates expression. Overall, study advances our understanding transcriptional regulation pest attacks, providing new insights into ecological significance under biotic stress.

Язык: Английский

Процитировано

0