Molecular requirements for C. elegans transgenerational epigenetic inheritance of pathogen avoidance DOI Creative Commons
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

и другие.

eLife, Год журнала: 2025, Номер 14

Опубликована: Май 2, 2025

Bacteria are Caenorhabditis elegans’ food, and worms naturally attracted to many bacteria, including pathogenic Pseudomonas , preferring PA14 over laboratory Escherichia coli (OP50). Despite this natural attraction PA14, prior exposure causes the instead avoid PA14. This behavioral switch can happen quickly – even within duration of choice assay. We show that accurate assessment animals’ true first requires use a paralytic (azide) trap at their initial choice, preventing from avoidance assay period. previously discovered C. elegans 25°C plate-grown 20°C for 24 hr not only leads avoidance, but also four generations naïve progeny avoiding while other paradigms cause P0 and/or F1 avoidance. showed transgenerational (P0-F4) epigenetic is mediated by P11, small RNA produced P11 both necessary sufficient TEI learned highly expressed in our standard growth conditions (25°C on surfaces), conditions, suggesting reported failure observe F2-F4 likely due absence expression experimenters’ conditions. Additionally, we tested ~35 genes involvement pathogen The conservation multiple components sRNA mechanism across strains species suggests behavior be physiologically important wild

Язык: Английский

Molecular requirements for C. elegans transgenerational epigenetic inheritance of pathogen avoidance DOI Creative Commons
Rachel Kaletsky, Rebecca S. Moore, Titas Sengupta

и другие.

eLife, Год журнала: 2025, Номер 14

Опубликована: Май 2, 2025

Bacteria are Caenorhabditis elegans’ food, and worms naturally attracted to many bacteria, including pathogenic Pseudomonas , preferring PA14 over laboratory Escherichia coli (OP50). Despite this natural attraction PA14, prior exposure causes the instead avoid PA14. This behavioral switch can happen quickly – even within duration of choice assay. We show that accurate assessment animals’ true first requires use a paralytic (azide) trap at their initial choice, preventing from avoidance assay period. previously discovered C. elegans 25°C plate-grown 20°C for 24 hr not only leads avoidance, but also four generations naïve progeny avoiding while other paradigms cause P0 and/or F1 avoidance. showed transgenerational (P0-F4) epigenetic is mediated by P11, small RNA produced P11 both necessary sufficient TEI learned highly expressed in our standard growth conditions (25°C on surfaces), conditions, suggesting reported failure observe F2-F4 likely due absence expression experimenters’ conditions. Additionally, we tested ~35 genes involvement pathogen The conservation multiple components sRNA mechanism across strains species suggests behavior be physiologically important wild

Язык: Английский

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