Cancer Science,
Год журнала:
2017,
Номер
108(3), С. 283 - 289
Опубликована: Янв. 8, 2017
Recently,
many
types
of
in
vitro
3-D
culture
systems
have
been
developed
to
recapitulate
the
vivo
growth
conditions
cancer.
The
cancer
methods
aim
preserve
biological
characteristics
original
tumors
better
than
conventional
2-D
monolayer
cultures,
and
include
tumor-derived
organoids,
spheroids,
organotypic
multicellular
tumor
spheroids.
differ
terms
cell
sources,
protocols
for
handling,
required
time
intervals.
Tumor-derived
spheroids
are
unique
because
they
purposed
enrichment
stem
cells
(CSCs)
or
with
cell-related
characteristics.
These
grown
as
floating
spheres
used
surrogate
evaluate
CSC-related
solid
vitro.
Because
eradication
CSCs
is
likely
be
clinical
importance
due
their
association
malignant
nature
cells,
such
tumorigenicity
chemoresistance,
investigation
may
provide
invaluable
clues
fight
against
Spheroid
cultures
established
from
cancers
including
glioma,
breast,
colon,
ovary,
prostate
cancers,
biochemical
investigated
by
research
groups.
In
addition
CSCs,
prove
instrumental
a
high-throughput
screening
platform
cultivation
found
circulation
body
fluids.
Journal of Hematology & Oncology,
Год журнала:
2022,
Номер
15(1)
Опубликована: Сен. 12, 2022
Abstract
Liquid
biopsies
are
increasingly
used
for
cancer
molecular
profiling
that
enables
a
precision
oncology
approach.
Circulating
extracellular
nucleic
acids
(cell-free
DNA;
cfDNA),
circulating
tumor
DNA
(ctDNA),
and
cells
(CTCs)
can
be
isolated
from
the
blood
other
body
fluids.
This
review
will
focus
on
current
technologies
clinical
applications
liquid
biopsies.
ctDNA/cfDNA
has
been
analyzed
using
many
techniques,
e.g.,
droplet
digital
polymerase
chain
reaction,
beads,
emulsion,
amplification,
magnetics
(BEAMing),
tagged-amplicon
deep
sequencing
(TAm-Seq),
personalized
by
(CAPP-Seq),
whole
genome
bisulfite
(WGBS-Seq),
exome
(WES),
(WGS).
CTCs
have
biomarker-based
cell
capture,
positive
or
negative
enrichment
based
biophysical
properties.
being
exploited
in
variety
of
applications:
differentiating
unique
immune
checkpoint
blockade
response
patterns
serial
samples;
predicting
baseline
biopsy
characteristics;
resistance
to
targeted
therapy
chemotherapy
as
well
immunotherapy,
including
CAR-T
cells,
sampling;
assessing
shed
multiple
metastatic
sites;
potentially
actionable
alterations;
analyzing
prognosis
burden,
after
surgery;
interrogating
difficult-to
tumors;
detecting
at
early
stages.
The
latter
limited
small
amounts
tumor-derived
components
into
circulation;
furthermore,
cfDNA
assessment
all
cancers
confounded
clonal
hematopoeisis
indeterminate
potential,
especially
elderly.
technically
more
difficult
isolate
cfDNA,
but
permit
functional
assays,
evaluation
CTC-derived
DNA,
RNA
proteins,
single-cell
analysis.
Blood
less
invasive
than
tissue
hence
amenable
collection,
which
provide
critical
information
real
time.
In
conclusion,
is
powerful
tool,
remarkable
advances
this
technology
impacted
aspects
oncology,
diagnosis
management
refractory
disease.
Future
research
may
fluids
beyond
blood,
such
ascites,
effusions,
urine,
cerebrospinal
fluid,
methylation
elements
exosomes.
EMBO Molecular Medicine,
Год журнала:
2014,
Номер
7(1), С. 1 - 11
Опубликована: Ноя. 14, 2014
Abstract
Cancer
metastasis
is
the
main
cause
of
cancer‐related
death,
and
dissemination
tumor
cells
through
blood
circulation
an
important
intermediate
step
that
also
exemplifies
switch
from
localized
to
systemic
disease.
Early
detection
characterization
circulating
(
CTC
s)
therefore
as
a
general
strategy
monitor
prevent
development
overt
metastatic
Furthermore,
sequential
analysis
s
can
provide
clinically
relevant
information
on
effectiveness
progression
therapies
(e.g.,
chemo‐,
hormonal,
or
targeted
with
antibodies
small
inhibitors).
Although
many
advances
have
been
made
regarding
molecular
s,
several
challenges
still
exist
limit
current
use
this
diagnostic
approach.
In
review,
we
discuss
biology
cell
dissemination,
technical
advances,
well
potential
clinical
implications
characterization.
Chemical Reviews,
Год журнала:
2021,
Номер
121(19), С. 12035 - 12105
Опубликована: Март 5, 2021
The
past
decade
has
witnessed
ongoing
progress
in
precision
medicine
to
improve
human
health.
As
an
emerging
diagnostic
technique,
liquid
biopsy
can
provide
real-time,
comprehensive,
dynamic
physiological
and
pathological
information
a
noninvasive
manner,
opening
new
window
for
medicine.
Liquid
depends
on
the
sensitive
reliable
detection
of
circulating
targets
(e.g.,
cells,
extracellular
vesicles,
proteins,
microRNAs)
from
body
fluids,
performance
which
is
largely
governed
by
recognition
ligands.
Aptamers
are
single-stranded
functional
oligonucleotides,
capable
folding
into
unique
tertiary
structures
bind
their
with
superior
specificity
affinity.
Their
mature
evolution
procedure,
facile
modification,
affinity
regulation,
as
well
versatile
structural
design
engineering,
make
aptamers
ideal
ligands
biopsy.
In
this
review,
we
present
broad
overview
aptamer-based
techniques
We
begin
recent
advances
aptamer
selection,
followed
summary
state-of-the-art
strategies
multivalent
assembly
interface
modification.
will
further
describe
micro-/nanoisolation
platforms,
aptamer-enabled
release
methods,
aptamer-assisted
signal
amplification
strategies.
Finally,
our
perspectives
regarding
opportunities
challenges
