Tumor‐derived spheroids: Relevance to cancer stem cells and clinical applications DOI Creative Commons
Tatsuya Ishiguro,

Hirokazu Ohata,

Ai Sato

и другие.

Cancer Science, Год журнала: 2017, Номер 108(3), С. 283 - 289

Опубликована: Янв. 8, 2017

Recently, many types of in vitro 3-D culture systems have been developed to recapitulate the vivo growth conditions cancer. The cancer methods aim preserve biological characteristics original tumors better than conventional 2-D monolayer cultures, and include tumor-derived organoids, spheroids, organotypic multicellular tumor spheroids. differ terms cell sources, protocols for handling, required time intervals. Tumor-derived spheroids are unique because they purposed enrichment stem cells (CSCs) or with cell-related characteristics. These grown as floating spheres used surrogate evaluate CSC-related solid vitro. Because eradication CSCs is likely be clinical importance due their association malignant nature cells, such tumorigenicity chemoresistance, investigation may provide invaluable clues fight against Spheroid cultures established from cancers including glioma, breast, colon, ovary, prostate cancers, biochemical investigated by research groups. In addition CSCs, prove instrumental a high-throughput screening platform cultivation found circulation body fluids.

Язык: Английский

The biology of circulating tumor cells DOI Open Access
Klaus Pantel, Michael R. Speicher

Oncogene, Год журнала: 2015, Номер 35(10), С. 1216 - 1224

Опубликована: Июнь 8, 2015

Язык: Английский

Процитировано

495
Ex Vivo Profiling of PD-1 Blockade Using Organotypic Tumor Spheroids DOI Open Access
Russell W. Jenkins, Amir Reza Aref, Patrick H. Lizotte

и другие.

Cancer Discovery, Год журнала: 2017, Номер 8(2), С. 196 - 215

Опубликована: Ноя. 4, 2017

Ex vivo systems that incorporate features of the tumor microenvironment and model dynamic response to immune checkpoint blockade (ICB) may facilitate efforts in precision immuno-oncology development effective combination therapies. Here, we demonstrate ability interrogate ex ICB using murine- patient-derived organotypic spheroids (MDOTS/PDOTS). MDOTS/PDOTS isolated from mouse human tumors retain autologous lymphoid myeloid cell populations respond short-term three-dimensional microfluidic culture. Response resistance was recapitulated MDOTS derived established immunocompetent models. profiling demonstrated TBK1/IKKε inhibition enhanced PD-1 blockade, which effectively predicted Systematic secreted cytokines PDOTS captured key associated with blockade. Thus, represents a novel platform evaluate murine models as well clinically relevant patient specimens.Significance: Resistance remains challenge for many patients, biomarkers guide treatment are lacking. feasibility microenvironment, develop therapeutic combinations, efforts. Cancer Discov; 8(2); 196-215. ©2017 AACR.See related commentary by Balko Sosman, p. 143See article Deng et al., 216This is highlighted In This Issue feature, 127.

Язык: Английский

Процитировано

474
Unravelling tumour heterogeneity by single-cell profiling of circulating tumour cells DOI
Laura Keller, Klaus Pantel

Nature reviews. Cancer, Год журнала: 2019, Номер 19(10), С. 553 - 567

Опубликована: Авг. 27, 2019

Язык: Английский

Процитировано

470
Liquid Biopsies in Cancer Diagnosis, Monitoring, and Prognosis DOI
Gabriele De Rubis,

Sabna Rajeev Krishnan,

Mary Bebawy

и другие.

Trends in Pharmacological Sciences, Год журнала: 2019, Номер 40(3), С. 172 - 186

Опубликована: Фев. 5, 2019

Язык: Английский

Процитировано

463
Tumor‐derived spheroids: Relevance to cancer stem cells and clinical applications DOI Creative Commons
Tatsuya Ishiguro,

Hirokazu Ohata,

Ai Sato

и другие.

Cancer Science, Год журнала: 2017, Номер 108(3), С. 283 - 289

Опубликована: Янв. 8, 2017

Recently, many types of in vitro 3-D culture systems have been developed to recapitulate the vivo growth conditions cancer. The cancer methods aim preserve biological characteristics original tumors better than conventional 2-D monolayer cultures, and include tumor-derived organoids, spheroids, organotypic multicellular tumor spheroids. differ terms cell sources, protocols for handling, required time intervals. Tumor-derived spheroids are unique because they purposed enrichment stem cells (CSCs) or with cell-related characteristics. These grown as floating spheres used surrogate evaluate CSC-related solid vitro. Because eradication CSCs is likely be clinical importance due their association malignant nature cells, such tumorigenicity chemoresistance, investigation may provide invaluable clues fight against Spheroid cultures established from cancers including glioma, breast, colon, ovary, prostate cancers, biochemical investigated by research groups. In addition CSCs, prove instrumental a high-throughput screening platform cultivation found circulation body fluids.

Язык: Английский

Процитировано

440