ACS Nano,
Год журнала:
2020,
Номер
14(7), С. 8866 - 8874
Опубликована: Июнь 23, 2020
Carbon
nanotube
(CNT)
field-effect
transistor
(FET)-based
biosensors
have
shown
great
potential
for
ultrasensitive
biomarker
detection,
but
challenges
remain,
which
include
unsatisfactory
sensitivity,
difficulty
in
stable
functionalization,
incompatibility
with
scalable
fabrication,
and
nonuniform
performance.
Here,
we
describe
ultrasensitive,
label-free,
FET
built
on
polymer-sorted
high-purity
semiconducting
CNT
films
wafer-scale
fabrication
high
uniformity.
With
a
floating
gate
(FG)
structure
using
an
ultrathin
Y2O3
high-κ
dielectric
layer,
the
show
amplified
response
improved
sensitivity
compared
those
sensors
without
Y2O3,
is
attributed
to
chemical
gate-coupling
effect
dominating
sensor
response.
The
FG-FETs
are
modified
selectively
detect
specific
disease
biomarkers,
namely,
DNA
sequences
microvesicles,
theoretical
record
detection
limits
as
low
60
aM
6
particles/mL,
respectively.
Furthermore,
exhibit
highly
uniform
performance
over
4
in.
wafer
well
superior
bias
stress
stability.
FG
could
be
extended
universal
biosensor
platform
of
multiple
biological
molecules
applied
integrated
multiplexed
all
CNT-FET-based
architectures.
ACS Nano,
Год журнала:
2023,
Номер
17(6), С. 5211 - 5295
Опубликована: Март 9, 2023
Humans
rely
increasingly
on
sensors
to
address
grand
challenges
and
improve
quality
of
life
in
the
era
digitalization
big
data.
For
ubiquitous
sensing,
flexible
are
developed
overcome
limitations
conventional
rigid
counterparts.
Despite
rapid
advancement
bench-side
research
over
last
decade,
market
adoption
remains
limited.
To
ease
expedite
their
deployment,
here,
we
identify
bottlenecks
hindering
maturation
propose
promising
solutions.
We
first
analyze
achieving
satisfactory
sensing
performance
for
real-world
applications
then
summarize
issues
compatible
sensor-biology
interfaces,
followed
by
brief
discussions
powering
connecting
sensor
networks.
Issues
en
route
commercialization
sustainable
growth
sector
also
analyzed,
highlighting
environmental
concerns
emphasizing
nontechnical
such
as
business,
regulatory,
ethical
considerations.
Additionally,
look
at
future
intelligent
sensors.
In
proposing
a
comprehensive
roadmap,
hope
steer
efforts
towards
common
goals
guide
coordinated
development
strategies
from
disparate
communities.
Through
collaborative
efforts,
scientific
breakthroughs
can
be
made
sooner
capitalized
betterment
humanity.
Chemical Reviews,
Год журнала:
2019,
Номер
119(1), С. 478 - 598
Опубликована: Янв. 3, 2019
Electrically–transduced
sensors,
with
their
simplicity
and
compatibility
standard
electronic
technologies,
produce
signals
that
can
be
efficiently
acquired,
processed,
stored,
analyzed.
Two
dimensional
(2D)
nanomaterials,
including
graphene,
phosphorene
(BP),
transition
metal
dichalcogenides
(TMDCs),
others,
have
proven
to
attractive
for
the
fabrication
of
high–performance
electrically-transduced
chemical
sensors
due
remarkable
physical
properties
originating
from
2D
structure.
This
review
highlights
advances
in
sensing
rely
on
materials.
The
structural
components
such
are
described,
underlying
operating
principles
different
types
architectures
discussed.
features,
properties,
surface
chemistry
nanostructures
dictate
performance
reviewed.
Key
application
materials,
both
a
historical
analytical
perspective,
summarized
four
groups
analytes:
gases,
volatile
compounds,
ions,
biomolecules.
is
discussed
context
molecular
design,
structure–property
relationships,
device
technology.
outlook
challenges
opportunities
nanomaterials
future
development
also
presented.
Angewandte Chemie International Edition,
Год журнала:
2019,
Номер
58(48), С. 17399 - 17405
Опубликована: Окт. 1, 2019
An
accurate,
rapid,
and
cost-effective
biosensor
for
the
quantification
of
disease
biomarkers
is
vital
development
early-diagnostic
point-of-care
systems.
The
recent
discovery
trans-cleavage
property
CRISPR
type
V
effectors
makes
a
potential
high-accuracy
bio-recognition
tool.
Herein,
CRISPR-Cas12a
(cpf1)
based
electrochemical
(E-CRISPR)
reported,
which
more
portable
than
optical-transduction-based
biosensors.
Through
optimizing
in
vitro
activity
Cas12a,
E-CRIPSR
was
used
to
detect
viral
nucleic
acids,
including
human
papillomavirus
16
(HPV-16)
parvovirus
B19
(PB-19),
with
picomolar
sensitivity.
aptamer-based
E-CRISPR
cascade
further
designed
detection
transforming
growth
factor
β1
(TGF-β1)
protein
clinical
samples.
As
demonstrated,
could
enable
portable,
diagnostic
Angewandte Chemie International Edition,
Год журнала:
2020,
Номер
60(5), С. 2221 - 2231
Опубликована: Апрель 13, 2020
Abstract
The
advent
of
SELEX
(systematic
evolution
ligands
by
exponential
enrichment)
technology
has
shown
the
ability
to
evolve
artificial
with
affinity
and
specificity
able
meet
growing
clinical
demand
for
probes
that
can,
example,
distinguish
between
target
leukemia
cells
other
cancer
within
matrix
heterogeneity,
which
characterizes
cells.
Though
antibodies
are
conventional
ideal
choice
as
a
molecular
recognition
tool
many
applications,
aptamers
complement
use
due
unique
advantages,
such
small
size,
low
cost,
facile
chemical
modification.
This
Minireview
will
focus
on
novel
applications
SELEX,
well
opportunities
develop
tools
future
needs
in
biomedicine.
Nature Reviews Methods Primers,
Год журнала:
2021,
Номер
1(1)
Опубликована: Окт. 7, 2021
Electrolyte-gated
transistors
(EGTs),
capable
of
transducing
biological
and
biochemical
inputs
into
amplified
electronic
signals
stably
operating
in
aqueous
environments,
have
emerged
as
fundamental
building
blocks
bioelectronics.
In
this
Primer,
the
different
EGT
architectures
are
described
with
mechanisms
underpinning
their
functional
operation,
providing
insight
key
experiments
including
necessary
data
analysis
validation.
Several
organic
inorganic
materials
used
structures
fabrication
approaches
for
an
optimal
experimental
design
presented
compared.
The
bio-layers
and/or
biosystems
integrated
or
interfaced
to
EGTs,
self-organization
self-assembly
strategies,
reviewed.
Relevant
promising
applications
discussed,
two-dimensional
three-dimensional
cell
monitoring,
ultra-sensitive
biosensors,
electrophysiology,
synaptic
neuromorphic
bio-interfaces,
prosthetics
robotics.
Advantages,
limitations
possible
optimizations
also
surveyed.
Finally,
current
issues
future
directions
further
developments
discussed.
(EGTs)
bioelectronics,
which
transduce
electrical
signals.
This
Primer
examines
mechanism
operation
practical
considerations
related
wide
range
applications.
Angewandte Chemie International Edition,
Год журнала:
2021,
Номер
60(31), С. 16800 - 16823
Опубликована: Фев. 9, 2021
Abstract
Aptamers
are
short
oligonucleotides
isolated
in
vitro
from
randomized
libraries
that
can
bind
to
specific
molecules
with
high
affinity,
and
offer
a
number
of
advantages
relative
antibodies
as
biorecognition
elements
biosensors.
However,
it
remains
difficult
labor‐intensive
develop
aptamer‐based
sensors
for
small‐molecule
detection.
Here,
we
review
the
challenges
advances
isolation
characterization
small‐molecule‐binding
DNA
aptamers
their
use
sensors.
First,
discuss
methodologies
aptamers,
provide
guidance
on
selecting
appropriate
strategy
generating
optimal
binding
properties
given
application.
We
next
examine
techniques
characterizing
aptamer–target
structure.
Afterwards,
various
sensing
platforms
based
original
or
engineered
detection
applications.
Finally,
conclude
general
workflow
real‐world
