Nature Reviews Genetics, Год журнала: 2021, Номер 23(5), С. 298 - 314
Опубликована: Дек. 8, 2021
Язык: Английский
Nature reviews. Cancer, Год журнала: 2020, Номер 20(10), С. 555 - 572
Опубликована: Авг. 10, 2020
Язык: Английский
Процитировано
1018
Nature, Год журнала: 2022, Номер 607(7920), С. 799 - 807
Опубликована: Июль 20, 2022
The APOBEC3 family of cytosine deaminases has been implicated in some the most prevalent mutational signatures cancer1-3. However, a causal link between endogenous enzymes and human cancer genomes not established, leaving mechanisms mutagenesis poorly understood. Here, to investigate mutagenesis, we deleted genes from cell lines that naturally generate APOBEC3-associated over time4. Analysis non-clustered clustered across whole-genome sequences 251 breast, bladder lymphoma line clones revealed APOBEC3A deletion diminished signatures. Deletion both APOBEC3B further decreased mutation burdens, without eliminating them. increased protein levels, activity APOBEC3A-mediated lines. uracil glycosylase UNG was required for APOBEC3-mediated transversions, whereas loss translesion polymerase REV1 overall burdens. Together, these data represent direct evidence cells. Our results identify as main driver mutations, indicate can restrain APOBEC3A-dependent while contributing its own smaller burdens dissect translate activities into distinct
Язык: Английский
Процитировано
184
Nature Reviews Genetics, Год журнала: 2022, Номер 23(8), С. 505 - 518
Опубликована: Март 7, 2022
The AID/APOBEC polynucleotide cytidine deaminases have historically been classified as either DNA mutators or RNA editors based on their first identified nucleic acid substrate preference. can generate functional diversity at antibody genes but also cause genomic instability in cancer. informational the transcriptome of innate immune cells, and cancer cells. Members both classes act antiviral restriction factors. Recent structural work has illuminated differences similarities between enzymes that catalyse mutation, editing both, suggesting strict classification members this family should be reconsidered. As many these employed for targeted genome (or transcriptome) editing, a more holistic understanding will help improve design therapeutically relevant programmable base editors. In Perspective, Pecori et al. provide an overview deaminase family, discussing key features, how they contribute to viral tumour evolution harnessed (potentially therapeutic) base-editing purposes.
Язык: Английский
Процитировано
180
Molecular Cell, Год журнала: 2022, Номер 82(2), С. 348 - 388
Опубликована: Янв. 1, 2022
Язык: Английский
Процитировано
157
Cancer Letters, Год журнала: 2020, Номер 496, С. 104 - 116
Опубликована: Окт. 7, 2020
Язык: Английский
Процитировано
154
Nature Reviews Genetics, Год журнала: 2021, Номер 22(8), С. 483 - 501
Опубликована: Март 24, 2021
Язык: Английский
Процитировано
143
BMC Genomics, Год журнала: 2022, Номер 23(1)
Опубликована: Фев. 15, 2022
The collective of somatic mutations in a genome represents record mutational processes that have been operative cell. These can be investigated by extracting relevant patterns from sequencing data.
Язык: Английский
Процитировано
124
Nature, Год журнала: 2022, Номер 602(7897), С. 510 - 517
Опубликована: Фев. 9, 2022
Clustered somatic mutations are common in cancer genomes and previous analyses reveal several types of clustered single-base substitutions, which include doublet- multi-base substitutions
Язык: Английский
Процитировано
120
Nature Genetics, Год журнала: 2021, Номер 53(11), С. 1553 - 1563
Опубликована: Окт. 18, 2021
Язык: Английский
Процитировано
118
Nature, Год журнала: 2023, Номер 620(7973), С. 393 - 401
Опубликована: Июль 5, 2023
Язык: Английский
Процитировано
82