Past, present, and future of CRISPR genome editing technologies

Cell, Год журнала: 2024, Номер 187(5), С. 1076 - 1100

Опубликована: Фев. 1, 2024

Genome editing has been a transformative force in the life sciences and human medicine, offering unprecedented opportunities to dissect complex biological processes treat underlying causes of many genetic diseases. CRISPR-based technologies, with their remarkable efficiency easy programmability, stand at forefront this revolution. In Review, we discuss current state CRISPR gene technologies both research therapy, highlighting limitations that constrain them technological innovations have developed recent years address them. Additionally, examine summarize landscape applications context health therapeutics. Finally, outline potential future developments could shape coming years.

Язык: Английский

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Temporal modelling using single-cell transcriptomics

Nature Reviews Genetics, Год журнала: 2022, Номер 23(6), С. 355 - 368

Опубликована: Янв. 31, 2022

Язык: Английский

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Spatial biology of cancer evolution

Nature Reviews Genetics, Год журнала: 2022, Номер 24(5), С. 295 - 313

Опубликована: Дек. 9, 2022

Язык: Английский

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A mouse model with high clonal barcode diversity for joint lineage, transcriptomic, and epigenomic profiling in single cells

Cell, Год журнала: 2023, Номер 186(23), С. 5183 - 5199.e22

Опубликована: Окт. 17, 2023

Язык: Английский

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Lymph node metastasis in cancer progression: molecular mechanisms, clinical significance and therapeutic interventions

Signal Transduction and Targeted Therapy, Год журнала: 2023, Номер 8(1)

Опубликована: Сен. 27, 2023

Abstract Lymph nodes (LNs) are important hubs for metastatic cell arrest and growth, immune modulation, secondary dissemination to distant sites through a series of mechanisms, it has been proved that lymph node metastasis (LNM) is an essential prognostic indicator in many different types cancer. Therefore, oncologists understand the mechanisms tumor cells metastasize LNs, as well how LNM affects prognosis therapy patients with cancer order provide accurate disease assessment effective treatment strategies. In recent years, updates both basic clinical studies on application advanced medical technologies, much progress made understanding strategies diagnosis LNM. this review, current knowledge anatomical physiological characteristics molecular LNM, described. The significance summarized, including roles playing staging, prediction, selection various cancers. And novel exploration academic disputes recognition, diagnosis, therapeutic interventions LNs also discussed.

Язык: Английский

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KRT17 Promotes T-lymphocyte Infiltration Through the YTHDF2–CXCL10 Axis in Colorectal Cancer

Cancer Immunology Research, Год журнала: 2023, Номер 11(7), С. 875 - 894

Опубликована: Май 2, 2023

Poor infiltration of T lymphocytes has been regarded as a crucial mechanism tumor immune escape. Here, we demonstrate protective role KRT17 in colorectal cancer, where reversed the immunosuppressive microenvironment by increasing T-lymphocyte infiltration. High-throughput RNA sequencing suggested that was significantly upregulated deficient mismatch repair (dMMR) tumors compared with proficient (pMMR) tumors. In cancer cohort 446 cases, expression positively correlated better clinical outcomes. Krt17 overexpression decreased xenograft growth immune-competent mice. T-cell depletion murine model showed presence necessary for Krt17-mediated disruption tumorigenesis. Mass spectrometry and coimmunoprecipitation assays caused YTHDF2 degradation through ubiquitin-proteasome system. Through high-throughput immunoprecipitation sequencing, found CXCL10 target gene N6-methyladenosine (m6A) "reader" YTHDF2. synergized anti-PD-1 control an immunotherapy-resistant model. patients receiving pembrolizumab, high within responders. Collectively, elucidated critical to prevent These findings present new insights into potential therapeutic strategies effective markers immunotherapy reactivity against pMMR

Язык: Английский

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Single-cell sequencing to multi-omics: technologies and applications

Biomarker Research, Год журнала: 2024, Номер 12(1)

Опубликована: Сен. 27, 2024

Abstract Cells, as the fundamental units of life, contain multidimensional spatiotemporal information. Single-cell RNA sequencing (scRNA-seq) is revolutionizing biomedical science by analyzing cellular state and intercellular heterogeneity. Undoubtedly, single-cell transcriptomics has emerged one most vibrant research fields today. With optimization innovation technologies, intricate details concealed within cells are gradually unveiled. The combination scRNA-seq other multi-omics at forefront field. This involves simultaneously measuring various omics data individual cells, expanding our understanding across a broader spectrum dimensions. precisely captures aspects transcriptomes, immune repertoire, spatial information, temporal epitopes, in diverse contexts. In addition to depicting cell atlas normal or diseased tissues, it also provides cornerstone for studying differentiation development patterns, disease heterogeneity, drug resistance mechanisms, treatment strategies. Herein, we review traditional technologies outline latest advancements multi-omics. We summarize current status challenges applying biological clinical applications. Finally, discuss limitations potential strategies address them.

Язык: Английский

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High-resolution, noninvasive single-cell lineage tracing in mice and humans based on DNA methylation epimutations

Nature Methods, Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

Язык: Английский

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Single-cell ATAC and RNA sequencing reveal pre-existing and persistent cells associated with prostate cancer relapse

Nature Communications, Год журнала: 2021, Номер 12(1)

Опубликована: Сен. 6, 2021

Abstract Prostate cancer is heterogeneous and patients would benefit from methods that stratify those who are likely to respond systemic therapy. Here, we employ single-cell assays for transposase-accessible chromatin (ATAC) RNA sequencing in models of early treatment response resistance enzalutamide. In doing so, identify pre-existing treatment-persistent cell subpopulations possess regenerative potential when subjected treatment. We find distinct landscapes associated with enzalutamide linked alternative transcriptional programs. Transcriptional profiles characteristic persistent cells able the patients. Ultimately, show defining changes gene expression populations pre-clinical can reveal as yet unrecognized molecular predictors response. This suggests application high analytical resolution may powerfully inform clinical decision-making.

Язык: Английский

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Progression of Metastasis through Lymphatic System

Cells, Год журнала: 2021, Номер 10(3), С. 627 - 627

Опубликована: Март 12, 2021

Lymph nodes are the most common sites of metastasis in cancer patients. Nodal disease status provides great prognostic power, but how lymph node metastases should be treated is under debate. Thus, it important to understand mechanisms by which progress and they can targeted provide therapeutic benefits. In this review, we focus on delineating process cell migration through lymphatic vessels, survival draining further spread other distant organs. addition, emerging molecular targets potential strategies inhibit discussed.

Язык: Английский

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