
Current Plant Biology, Год журнала: 2024, Номер unknown, С. 100385 - 100385
Опубликована: Сен. 1, 2024
Язык: Английский
Current Plant Biology, Год журнала: 2024, Номер unknown, С. 100385 - 100385
Опубликована: Сен. 1, 2024
Язык: Английский
The Innovation, Год журнала: 2022, Номер 3(6), С. 100342 - 100342
Опубликована: Окт. 18, 2022
Язык: Английский
Процитировано
90Plant Biotechnology Journal, Год журнала: 2022, Номер 20(12), С. 2372 - 2388
Опубликована: Авг. 29, 2022
Summary Cotton fibre is a unicellular seed trichome, and lint initials per as factor determines yield. However, the mechanisms controlling initiation from ovule epidermis are not understood well enough. Here, with single‐cell RNA sequencing (scRNA‐seq), total of 14 535 cells were identified cotton outer integument Xu142_LF line at four developmental stages (1.5, 1, 0.5 days before anthesis day anthesis). Three major cell types, fibre, non‐fibre pigment layer then verified by in situ hybridization. A comparative analysis on scRNA‐seq data between Xu142 its fibreless mutant fl further confirmed cluster definition. The trajectory was reconstructed, differentiated 1 anthesis. Gene regulatory networks revealed spatiotemporal pattern core transcription factors, MYB25‐like HOX3 demonstrated played key roles commanders differentiation tip‐biased diffuse growth respectively. model for early development single proposed here, which sheds light deciphering mechanism plant trichome improvement
Язык: Английский
Процитировано
80Plant Communications, Год журнала: 2024, Номер 5(4), С. 100822 - 100822
Опубликована: Янв. 21, 2024
Plant genetic transformation strategies serve as essential tools for the engineering and advanced molecular breeding of plants. However, complicated operational protocol low efficiency current restrict modification most plant species. This paper describes development a Regenerative Activity-dependent in Planta Injection Delivery (RAPID) method based on active regeneration capacity In this method, Agrobacterium tumefaciens was delivered to meristems via injection inducing transfected nascence tissues. Stable transgenic plants were obtained by subsequent vegetative propagation positive The successfully applied with strong capacity, including different genotypes sweet potato (Ipomoea batatas), (Solanum tuberosum), bayhops pes-caprae). Compared traditional methods, RAPID has markedly high efficiency, shorter duration, does not require tissue culture procedures. therefore overcomes limitations methods achieving rapid planta transformation, can be potentially wide range species that are capable regeneration.
Язык: Английский
Процитировано
34The Plant Cell, Год журнала: 2024, Номер 36(4), С. 812 - 828
Опубликована: Янв. 17, 2024
Abstract Single-cell and single-nucleus RNA-sequencing technologies capture the expression of plant genes at an unprecedented resolution. Therefore, these are gaining traction in molecular developmental biology for elucidating transcriptional changes across cell types a specific tissue or organ, upon treatments, response to biotic abiotic stresses, between genotypes. Despite rapidly accelerating use technologies, collective standardized experimental analytical procedures support acquisition high-quality data sets still missing. In this commentary, we discuss common challenges associated with single-cell transcriptomics plants propose general guidelines improve reproducibility, quality, comparability, interpretation make readily available community fast-developing field research.
Язык: Английский
Процитировано
26Nature Communications, Год журнала: 2024, Номер 15(1)
Опубликована: Фев. 14, 2024
Abstract Climate change is driving extreme changes to the environment, posing substantial threats global food security and bioenergy. Given direct role of plant roots in mediating plant-environment interactions, engineering form function root systems their associated microbiota may mitigate these effects. Synthetic genetic circuits have enabled sophisticated control gene expression microbial for years a surge advances has heralded extension this approach multicellular species. Targeting tools affect structure, exudation, microbe activity on surfaces provide multiple strategies advancement climate-ready crops.
Язык: Английский
Процитировано
20International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(9), С. 4497 - 4497
Опубликована: Апрель 19, 2022
In recent years, advances in single-cell RNA sequencing (scRNA-seq) technologies have continued to change our views on biological systems by increasing the spatiotemporal resolution of analysis resolution. Application scRNA-seq plants enables comprehensive characterization both common and rare cell types states, uncovering new revealing how relate each other spatially developmentally. This review provides an overview methodologies, highlights application plant science, justifies why is a master player sequencing, explains role transcriptomics environmental stress adaptation, alongside challenges prospects transcriptomics. Collectively, we put forward central research.
Язык: Английский
Процитировано
51The Plant Journal, Год журнала: 2022, Номер 110(6), С. 1551 - 1563
Опубликована: Апрель 15, 2022
SUMMARY Single‐cell sequencing approaches reveal the intracellular dynamics of individual cells and answer biological questions with high‐dimensional catalogs millions cells, including genomics, transcriptomics, chromatin accessibility, epigenomics, proteomics data across species. These emerging yet thriving technologies have been fully embraced by field plant biology, a constantly expanding portfolio applications. Here, we introduce current technical advances used for single‐cell omics, especially genome transcriptome sequencing. Firstly, overview methods protoplast nucleus isolation amplification. Subsequently, use well‐executed benchmarking studies to highlight made through application omics techniques. Looking forward, offer glimpse additional hurdles future opportunities that will broad adoption revolutionary perspectives in biology.
Язык: Английский
Процитировано
47Science Advances, Год журнала: 2023, Номер 9(27)
Опубликована: Июль 7, 2023
Plants can regenerate their bodies via de novo establishment of shoot apical meristems (SAMs) from pluripotent callus. Only a small fraction callus cells is eventually specified into SAMs but the molecular mechanisms underlying fate specification remain obscure. The expression WUSCHEL (WUS) an early hallmark SAM acquisition. Here, we show that WUS paralog, WUSCHEL-RELATED HOMEOBOX 13 (WOX13), negatively regulates formation in Arabidopsis thaliana . WOX13 promotes non-meristematic cell transcriptional repression and other regulators activation wall modifiers. Our Quartz-Seq2–based single transcriptome revealed plays key roles determining cellular identity population. We propose reciprocal inhibition between mediates critical determination population, which has major impact on regeneration efficiency.
Язык: Английский
Процитировано
42The Plant Cell, Год журнала: 2024, Номер 36(5), С. 1777 - 1790
Опубликована: Янв. 8, 2024
Abstract Crown roots are the main components of root systems in cereals. Elucidating mechanisms crown formation is instrumental for improving nutrient absorption, stress tolerance, and yield cereal crops. Several members WUSCHEL-related homeobox (WOX) lateral organ boundaries domain (LBD) transcription factor families play essential roles controlling development rice (Oryza sativa). However, functional relationships among these factors regulating genes involved remain unclear. Here, we identified LBD16 as an additional regulator development. We showed that a direct downstream target WOX11, key rice. Our results indicated WOX11 enhances by binding to its promoter recruiting interaction partner JMJ706, demethylase removes histone H3 lysine 9 dimethylation (H3K9me2) from locus. In addition, established interacts with thereby impairing JMJ706–WOX11 complex repressing own transcriptional activity. Together, our reveal feedback system orchestrate rice, which acts molecular rheostat.
Язык: Английский
Процитировано
17PLANT PHYSIOLOGY, Год журнала: 2024, Номер 194(4), С. 2472 - 2490
Опубликована: Янв. 13, 2024
Abstract LATERAL ORGAN BOUNDARIES DOMAIN/ASYMMETRIC LEAVES2-LIKEs (LBDs/ASLs) are plant-specific transcription factors that function downstream of auxin-regulated lateral root (LR) formation. Our previous research found PpLBD16 positively regulates peach (Prunus persica) LR However, the regulatory network and target genes still largely unknown. Here, we constructed a homologous overexpression line silenced line. We overexpressing promoted initiation, while silencing inhibited Through RNA sequencing (RNA-seq) analysis roots from lines, discovered regulated by were closely related to cell wall synthesis degradation, ion/substance transport, ion binding homeostasis. To further detect motifs potential PpLBD16, performed DNA-affinity purification (DAP-seq) in vitro. preferentially bound CCNGAAANNNNGG (MEME-1), [C/T]TTCT[C/T][T/C] (MEME-2), GCGGCGG (ABR1) motifs. By combined RNA-seq DAP-seq data, screened candidate for PpLBD16. demonstrated activated modification-related EXPANSIN-B2 (PpEXPB2) SUBTILISIN-LIKE PROTEASE 1.7 (PpSBT1.7), transport-related gene CYCLIC NUCLEOTIDE-GATED ION CHANNEL 1 (PpCNGC1) polyphenol oxidase (PPO)-encoding PpPPO, thereby controlling organogenesis promoting Moreover, our results displayed its involved primordia development. Overall, this work reveals providing insights into molecular LBD16-mediated
Язык: Английский
Процитировано
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