Frontiers in Bioengineering and Biotechnology,
Год журнала:
2025,
Номер
13
Опубликована: Март 6, 2025
In
the
field
of
wound
treatment,
chronic
wounds
pose
a
significant
burden
on
medical
system,
affecting
millions
patients
annually.
Current
treatment
methods
often
fall
short
in
promoting
effective
healing,
highlighting
need
for
innovative
approaches.
Electrospinning,
technique
that
has
garnered
increasing
attention
recent
years,
shows
promise
care
due
to
its
unique
characteristics
and
advantages.
Recent
studies
have
explored
use
electrospun
nanofibers
demonstrating
their
efficacy
cell
growth
tissue
regeneration.
Researchers
investigated
various
materials
electrospinning,
including
polymers,
ceramics,
carbon
nanotubes
(CNTs),
metals.
Hydrogel,
as
biomaterial
been
widely
studied
matrix.
When
combined
with
it
can
be
used
develop
dressings
multiple
functions.
This
article
is
review
application
electrospinning
technology
treatment.
It
introduces
current
research
status
areas
pathophysiology,
preparation
technology,
dressing
development,
hoping
provide
references
directions
future
research.
Signal Transduction and Targeted Therapy,
Год журнала:
2023,
Номер
8(1)
Опубликована: Фев. 17, 2023
Abstract
Having
a
hypoxic
microenvironment
is
common
and
salient
feature
of
most
solid
tumors.
Hypoxia
has
profound
effect
on
the
biological
behavior
malignant
phenotype
cancer
cells,
mediates
effects
chemotherapy,
radiotherapy,
immunotherapy
through
complex
mechanisms,
closely
associated
with
poor
prognosis
in
various
patients.
Accumulating
studies
have
demonstrated
that
normalization
tumor
vasculature,
nanoparticle
carriers
biocarriers
can
effectively
increase
oxygen
concentration
microenvironment,
improve
drug
delivery
efficacy
radiotherapy.
They
also
infiltration
innate
adaptive
anti-tumor
immune
cells
to
enhance
immunotherapy.
Furthermore,
drugs
targeting
key
genes
hypoxia,
including
hypoxia
tracers,
hypoxia-activated
prodrugs,
hypoxia-inducible
factors
downstream
targets,
be
used
for
visualization
quantitative
analysis
antitumor
activity.
However,
relationship
between
an
area
research
requires
further
exploration.
Here,
we
investigated
potential
development
cancer,
changes
signaling
pathways
occur
adapt
environments,
mechanisms
hypoxia-induced
tolerance,
chemotherapeutic
enhanced
radiation
as
well
insights
applications
therapy.
Chronic
hard-to-heal
wounds
draw
great
attention
worldwide,
as
their
treatments
are
limited
by
infections
and
hypoxia.
Inspired
the
natural
oxygen
production
capacity
of
algae
competitive
advantage
beneficial
bacteria
over
other
microbes,
we
presented
a
living
microecological
hydrogel
(LMH)
with
functionalized
Chlorella
Bacillus
subtilis
encapsulation
to
realize
continuous
delivery
anti-infections
for
promoting
chronic
wound
healing.
As
consisted
thermosensitive
Pluronic
F-127
wet-adhesive
polydopamine,
LMH
could
keep
liquid
at
low
temperature
while
quickly
solidifying
tightly
adhering
bed.
It
was
demonstrated
that
optimizing
proportion
encapsulated
microorganism,
continuously
produce
relieve
hypoxia
support
proliferation
B.
,
eliminate
colonized
pathogenic
bacteria.
Thus,
substantially
promoted
healing
infected
diabetic
wounds.
These
features
make
valuable
practical
clinical
applications.
Journal of genetics and genomics/Journal of Genetics and Genomics,
Год журнала:
2023,
Номер
50(9), С. 625 - 640
Опубликована: Март 27, 2023
The
ability
to
explore
life
kingdoms
is
largely
driven
by
innovations
and
breakthroughs
in
technology,
from
the
invention
of
microscope
350
years
ago
recent
emergence
single-cell
sequencing,
which
scientific
community
has
been
able
visualize
at
an
unprecedented
resolution.
Most
recently,
Spatially
Resolved
Transcriptomics
(SRT)
technologies
have
filled
gap
probing
spatial
or
even
three-dimensional
organization
molecular
foundation
behind
mysteries
life,
including
origin
different
cellular
populations
developed
totipotent
cells
human
diseases.
In
this
review,
we
introduce
progress
challenges
on
SRT
perspectives
bioinformatic
tools,
as
well
representative
applications.
With
currently
fast-moving
promising
results
early
adopted
research
projects,
can
foresee
bright
future
such
new
tools
understanding
most
profound
analytical
level.
Cell,
Год журнала:
2023,
Номер
186(10), С. 2127 - 2143.e22
Опубликована: Апрель 24, 2023
Pathogen
infection
and
tissue
injury
are
universal
insults
that
disrupt
homeostasis.
Innate
immunity
senses
microbial
infections
induces
cytokines/chemokines
to
activate
resistance
mechanisms.
Here,
we
show
that,
in
contrast
most
pathogen-induced
cytokines,
interleukin-24
(IL-24)
is
predominately
induced
by
barrier
epithelial
progenitors
after
independent
of
microbiome
or
adaptive
immunity.
Moreover,
Il24
ablation
mice
impedes
not
only
epidermal
proliferation
re-epithelialization
but
also
capillary
fibroblast
regeneration
within
the
dermal
wound
bed.
Conversely,
ectopic
IL-24
induction
homeostatic
epidermis
triggers
global
epithelial-mesenchymal
repair
responses.
Mechanistically,
expression
depends
upon
both
IL24-receptor/STAT3
signaling
hypoxia-stabilized
HIF1α,
which
converge
following
trigger
autocrine
paracrine
involving
IL-24-mediated
receptor
metabolic
regulation.
Thus,
parallel
innate
immune
sensing
pathogens
resolve
infections,
stem
cells
sense
signals
orchestrate
repair.
Immunity,
Год журнала:
2023,
Номер
56(1), С. 78 - 92.e6
Опубликована: Янв. 1, 2023
Tissue
repair
processes
maintain
proper
organ
function
following
mechanical
or
infection-related
damage.
In
addition
to
antibacterial
properties,
mucosal
associated
invariant
T
(MAIT)
cells
express
a
tissue
transcriptomic
program
and
promote
skin
wound
healing
when
expanded.
Herein,
we
use
human-like
mouse
model
of
full-thickness
excision
assess
the
underlying
mechanisms
MAIT
cell
function.
Single-cell
RNA
sequencing
analysis
suggested
that
already
at
steady
state.
Following
excision,
promoted
keratinocyte
proliferation,
thereby
accelerating
healing.
Using
grafts,
parabiosis,
adoptive
transfer
experiments,
show
migrated
into
in
receptor
(TCR)-independent
but
CXCR6
chemokine
receptor-dependent
manner.
Amphiregulin
secreted
by
Expression
was
probably
independent
sustained
TCR
stimulation.
Overall,
our
study
provides
mechanistic
insights
skin.
Science Immunology,
Год журнала:
2023,
Номер
8(84)
Опубликована: Июнь 2, 2023
Whereas
the
cellular
and
molecular
features
of
human
inflammatory
skin
diseases
are
well
characterized,
their
tissue
context
systemic
impact
remain
poorly
understood.
We
thus
profiled
psoriasis
(PsO)
as
a
prototypic
immune-mediated
condition
with
high
predilection
for
extracutaneous
involvement.
Spatial
transcriptomics
(ST)
analyses
25
healthy,
active
lesion,
clinically
uninvolved
biopsies
integration
public
single-cell
data
revealed
marked
differences
in
immune
microniches
between
healthy
inflamed
skin.
Tissue-scale
cartography
further
identified
core
disease
across
all
lesions,
including
emergence
an
suprabasal
epidermal
state
presence
B
lymphocytes
lesional
Both
distal
nonlesional
samples
were
stratified
by
severity
not
disease.
This
segregation
was
driven
macrophage-,
fibroblast-,
lymphatic-enriched
spatial
regions
gene
signatures
associated
metabolic
dysfunction.
Together,
these
findings
suggest
that
mild
severe
forms
PsO
have
distinct
may
profoundly
alter
composition
unaffected
sites.
In
addition,
our
study
provides
valuable
resource
research
community
to
organization
Science Immunology,
Год журнала:
2024,
Номер
9(96)
Опубликована: Июнь 7, 2024
During
ontogeny,
γδ
T
cells
emerge
from
the
thymus
and
directly
seed
peripheral
tissues
for
in
situ
immunity.
However,
their
functional
role
humans
has
largely
been
defined
blood.
Here,
we
analyzed
phenotype,
transcriptome,
function,
repertoire
of
human
blood
mucosal
lymphoid
176
donors
across
life
span,
revealing
distinct
profiles
children
compared
with
adults.
In
early
life,
clonally
diverse
Vδ1
subsets
predominate
tissues,
comprising
naïve
differentiated
effector
tissue
repair
functions,
whereas
cytolytic
Vδ2
populate
blood,
spleen,
lungs.
With
age,
exhibit
clonal
expansions
elevated
signatures,
which
are
disseminated
sites.
adults,
enriched
express
residency
profiles.
Thus,
antigenic
exposures
over
childhood
drive
evolution
compartmentalization
cells,
leading
to
age-dependent
roles
