Cells,
Год журнала:
2023,
Номер
12(8), С. 1148 - 1148
Опубликована: Апрель 13, 2023
Developmental
patterning
is
essential
for
regulating
cellular
events
such
as
axial
patterning,
segmentation,
tissue
formation,
and
organ
size
determination
during
embryogenesis.
Understanding
the
mechanisms
remains
a
central
challenge
fundamental
interest
in
developmental
biology.
Ion-channel-regulated
bioelectric
signals
have
emerged
player
of
mechanism,
which
may
interact
with
morphogens.
Evidence
from
multiple
model
organisms
reveals
roles
bioelectricity
embryonic
development,
regeneration,
cancers.
The
Zebrafish
second
most
used
vertebrate
model,
next
to
mouse
model.
zebrafish
has
great
potential
elucidating
functions
due
many
advantages
external
transparent
early
embryogenesis,
tractable
genetics.
Here,
we
review
genetic
evidence
mutants
fin-size
pigment
changes
related
ion
channels
bioelectricity.
In
addition,
cell
membrane
voltage
reporting
chemogenetic
tools
that
already
been
or
be
implemented
models.
Finally,
new
perspectives
opportunities
research
are
discussed.
Genetically
encoded
fluorescent
voltage
indicators
are
ideally
suited
to
reveal
the
millisecond-scale
interactions
among
and
between
targeted
cell
populations.
However,
current
lack
requisite
sensitivity
for
in
vivo
multipopulation
imaging.
We
describe
next-generation
green
red
sensors,
Ace-mNeon2
VARNAM2,
their
reverse
response-polarity
variants
pAce
pAceR.
Our
enable
0.4-
1-kilohertz
recordings
from
>50
spiking
neurons
per
field
of
view
awake
mice
~30-minute
continuous
imaging
flies.
Using
dual-polarity
multiplexed
imaging,
we
uncovered
brain
state-dependent
antagonism
neocortical
somatostatin-expressing
(SST+)
vasoactive
intestinal
peptide-expressing
(VIP+)
interneurons
contributions
hippocampal
potentials
ensembles
with
distinct
axonal
projections.
By
combining
three
mutually
compatible
indicators,
performed
simultaneous
triple-population
These
approaches
will
empower
investigations
dynamic
interplay
neuronal
subclasses
at
single-spike
resolution.
Science,
Год журнала:
2023,
Номер
380(6651), С. 1270 - 1275
Опубликована: Июнь 22, 2023
The
development
of
voltage-sensitive
fluorescent
probes
suggests
fluorescence
lifetime
as
a
promising
readout
for
electrical
activity
in
biological
systems.
Existing
approaches
fail
to
achieve
the
speed
and
sensitivity
required
voltage
imaging
neuroscience
applications.
Here
we
demonstrate
that
wide-field
electro-optic
(EO-FLIM)
allows
at
kHz
frame
acquisition
rates,
spatially
resolving
action
potential
propagation
sub-threshold
neural
live
adult
Drosophila.
Lifetime
resolutions
$<
5$
ps
1
were
achieved
single
cell
recordings.
is
limited
by
photon
shot
noise
method
provides
strong
rejection
motion
artifacts
technical
sources.
Recordings
revealed
local
transmembrane
depolarizations,
two
types
spikes
with
distinct
lifetimes,
phase
locking
an
external
mechanical
stimulus.
Nature Methods,
Год журнала:
2023,
Номер
20(5), С. 735 - 746
Опубликована: Апрель 6, 2023
Abstract
High-speed
three-dimensional
(3D)
intravital
imaging
in
animals
is
useful
for
studying
transient
subcellular
interactions
and
functions
health
disease.
Light-field
microscopy
(LFM)
provides
a
computational
solution
snapshot
3D
with
low
phototoxicity
but
restricted
by
resolution
reconstruction
artifacts
induced
optical
aberrations,
motion
noise.
Here,
we
propose
virtual-scanning
LFM
(VsLFM),
physics-based
deep
learning
framework
to
increase
the
of
up
diffraction
limit
within
snapshot.
By
constructing
40
GB
high-resolution
scanning
dataset
across
different
species,
exploit
physical
priors
between
phase-correlated
angular
views
address
frequency
aliasing
problem.
This
enables
us
bypass
hardware
associated
artifacts.
show
that
VsLFM
achieves
ultrafast
diverse
processes
such
as
beating
heart
embryonic
zebrafish,
voltage
activity
Drosophila
brains
neutrophil
migration
mouse
liver
at
500
volumes
per
second.
Abstract
Almost
all
physiological
processes
of
animals
are
controlled
by
the
brain,
including
language,
cognitive,
memory,
learning,
emotion
and
so
forth.
Minor
brain
dysfunction
usually
leads
to
diseases
disorders.
Therefore,
it'
is
greatly
meaningful
urgent
for
scientists
have
a
better
understanding
structure
function.
Optical
approaches
can
provide
powerful
tools
imaging
modulating
brain.
In
particular,
optical
in
near‐infrared
(NIR)
window
(700–1700
nm)
exhibit
excellent
prosperities
deep
tissue
penetration
low
scattering
absorption
compared
with
those
visible
windows
(400–700
nm),
which
provides
promising
approach
develop
desired
methods
neuroimaging
neuromodulation
tissues.
this
review,
variable
types
NIR
light
neural
ions,
membrane
potential,
neurotransmitters,
other
critical
molecules
functions
summarized.
latest
breakthrough
research
regulation
NIR‐II
(1000–1700
highlighted.
Finally,
we
conclude
challenges
prospects
light‐based
both
basic
further
clinical
translation.
Nature,
Год журнала:
2024,
Номер
634(8036), С. 1141 - 1149
Опубликована: Июль 22, 2024
In
dynamic
environments,
animals
make
behavioural
decisions
on
the
basis
of
innate
valences
sensory
cues
and
information
learnt
about
these
across
multiple
timescales
Microbial
rhodopsin–derived
genetically
encoded
voltage
indicators
(GEVIs)
are
powerful
tools
for
mapping
bioelectrical
dynamics
in
cell
culture
and
live
animals.
Förster
resonance
energy
transfer
(FRET)–opsin
GEVIs
use
voltage-dependent
quenching
of
an
attached
fluorophore,
achieving
high
brightness,
speed,
sensitivity.
However,
the
sensitivity
most
FRET-opsin
has
been
reported
to
decrease
or
vanish
under
two-photon
(2P)
excitation.
Here,
we
investigated
photophysics
Voltron1
Voltron2.
We
found
that
previously
negative-going
sensitivities
both
came
from
photocycle
intermediates,
not
opsin
ground
states.
The
were
nonlinear
functions
illumination
intensity;
Voltron1,
reversed
sign
low-intensity
illumination.
Using
photocycle-optimized
2P
protocols,
demonstrate
imaging
with
Voltron2
barrel
cortex
a
mouse.
These
results
open
door
high-speed
vivo.
ACS Photonics,
Год журнала:
2024,
Номер
11(3), С. 874 - 891
Опубликована: Март 5, 2024
The
development
of
cloud
computing
and
artificial
intelligence
technology
has
increased
data
storage
demands,
causing
an
urgency
to
progress
nanophotonics-enabled
optical
storage.
Inspiration
can
be
taken
from
the
working
principles
brain's
memory
that
high
capacity
parallelism,
integration
between
processing
with
self-learning
ability,
low-power
consumption.
correlation
emerging
neuroscience
concept
cell
engrams
as
basic
units
nanophotonics
techniques
materials
aid
neuromorphic
enabled
by
toward
higher
throughput
lower
energy
In
this
perspective,
we
explore
feasibility
a
counterpart
biology
emulating
based
on
We
overview
materials,
well
challenges
opportunities
such
implementation
for
future
nanophotonics.
