Frontiers in Neuroscience,
Год журнала:
2022,
Номер
16
Опубликована: Ноя. 16, 2022
Radial
glia
is
a
cell
type
traditionally
associated
with
the
developing
nervous
system,
particularly
formation
of
cortical
layers
in
mammalian
brain.
Nonetheless,
some
these
cells,
or
closely
related
types,
called
radial
glia-like
cells
are
found
adult
central
system
structures,
functioning
as
neurogenic
progenitors
normal
homeostatic
maintenance
and
response
to
injury.
The
heterogeneity
nowadays
being
probed
molecular
tools,
primarily
by
expression
specific
genes
that
define
types.
Similar
markers
have
identified
non-vertebrate
organisms.
In
this
review,
we
focus
on
processes
during
homeostasis
We
highlight
our
results
using
model
echinoderm
Abstract
Accurately
defining
cell
boundaries
for
spatial
transcriptomics
is
technically
challenging.
The
current
major
approaches
are
nuclear
staining
or
mathematical
inference,
which
either
exclude
the
cytoplasm
determine
a
hypothetical
boundary.
Here,
new
method
introduced
boundaries:
labeling
membranes
using
genetically
coded
fluorescent
proteins,
allows
precise
indexing
of
sequencing
spots
and
transcripts
within
cells
on
sections.
Use
this
membrane‐based
greatly
increases
number
genes
captured
in
compared
to
nucleus‐based
methods;
numbers
increased
by
67%
119%
mouse
axolotl
livers,
respectively.
obtained
expression
profiles
more
consistent
with
single‐cell
RNA‐seq
data,
demonstrating
rational
clustering
apparent
type‐specific
markers.
Furthermore,
improved
resolution
achieved
better
identify
rare
types
elaborate
domains
brain
intestine.
In
addition
regular
cells,
accurate
recognition
multinucleated
lacking
nuclei
liver
achieved,
its
ability
analyze
complex
tissues
organs,
not
achievable
previous
methods.
This
study
provides
powerful
tool
improving
that
has
broad
potential
applications
biological
medical
sciences.
Spatial
transcriptomics
is
a
powerful
tool
that
integrates
molecular
data
with
spatial
information,
thereby
facilitating
deeper
comprehension
of
tissue
morphology
and
cellular
interactions.
In
our
study,
we
utilized
cutting-edge
transcriptome
sequencing
technology
to
explore
the
development
mouse
heart
construct
comprehensive
spatiotemporal
cell
atlas
early
murine
cardiac
development.
Through
analysis
this
atlas,
elucidated
organization
lineages
their
interactions
during
developmental
process.
Notably,
observed
dynamic
changes
in
gene
expression
within
fibroblasts
cardiomyocytes.
Moreover,
identified
critical
genes,
such
as
Igf2,
H19,
Tcap,
well
transcription
factors
Tcf12
Plagl1,
which
may
be
associated
loss
myocardial
regeneration
ability
addition,
successfully
marker
like
Adamts8
Bmp10,
can
distinguish
between
left
right
atria.
Our
study
provides
novel
insights
into
offers
valuable
resource
for
future
investigations
field
research,
highlighting
significance
understanding
complex
processes
organ
Frontiers in Neuroscience,
Год журнала:
2022,
Номер
16
Опубликована: Ноя. 16, 2022
Radial
glia
is
a
cell
type
traditionally
associated
with
the
developing
nervous
system,
particularly
formation
of
cortical
layers
in
mammalian
brain.
Nonetheless,
some
these
cells,
or
closely
related
types,
called
radial
glia-like
cells
are
found
adult
central
system
structures,
functioning
as
neurogenic
progenitors
normal
homeostatic
maintenance
and
response
to
injury.
The
heterogeneity
nowadays
being
probed
molecular
tools,
primarily
by
expression
specific
genes
that
define
types.
Similar
markers
have
identified
non-vertebrate
organisms.
In
this
review,
we
focus
on
processes
during
homeostasis
We
highlight
our
results
using
model
echinoderm
