Cell Reports Methods, Год журнала: 2025, Номер unknown, С. 100987 - 100987
Опубликована: Фев. 1, 2025
Язык: Английский
Nature Immunology, Год журнала: 2023, Номер 24(12), С. 1982 - 1993
Опубликована: Ноя. 27, 2023
Язык: Английский
Процитировано
68
Signal Transduction and Targeted Therapy, Год журнала: 2024, Номер 9(1)
Опубликована: Авг. 7, 2024
Multicellular organisms are composed of diverse cell types that must coordinate their behaviors through communication. Cell-cell communication (CCC) is essential for growth, development, differentiation, tissue and organ formation, maintenance, physiological regulation. Cells communicate direct contact or at a distance using ligand-receptor interactions. So cellular encompasses two processes: signal conduction generation intercellular transmission signals, transduction reception procession signals. Deciphering networks critical understanding metabolism. First, we comprehensively review the historical milestones in CCC studies, followed by detailed description mechanisms molecule importance main signaling pathways they mediate maintaining biological functions. Then systematically introduce series human diseases caused abnormalities progress clinical applications. Finally, summarize various methods monitoring interactions, including imaging, proximity-based chemical labeling, mechanical force analysis, downstream analysis strategies, single-cell technologies. These aim to illustrate how functions depend on these interactions complexity regulatory regulate crucial processes, homeostasis, immune responses diseases. In addition, this enhances our processes occur after cell-cell binding, highlighting its application discovering new therapeutic targets biomarkers related precision medicine. This collective provides foundation developing targeted drugs personalized treatments.
Язык: Английский
Процитировано
56
Cell, Год журнала: 2024, Номер 187(15), С. 3953 - 3972.e26
Опубликована: Июнь 24, 2024
Язык: Английский
Процитировано
42
Cell, Год журнала: 2024, Номер 187(17), С. 4488 - 4519
Опубликована: Авг. 1, 2024
Язык: Английский
Процитировано
35
Molecular Cancer, Год журнала: 2024, Номер 23(1)
Опубликована: Июль 27, 2024
Abstract Tumor-associated macrophages (TAMs) are pivotal in cancer progression, influencing tumor growth, angiogenesis, and immune evasion. This review explores the spatial temporal heterogeneity of TAMs within microenvironment (TME), highlighting their diverse subtypes, origins, functions. Advanced technologies such as single-cell sequencing multi-omics have elucidated intricate interactions between other TME components, revealing mechanisms behind recruitment, polarization, distribution. Key findings demonstrate that support vascularization, promote epithelial-mesenchymal transition (EMT), modulate extracellular matrix (ECM) remodeling, etc., thereby enhancing invasiveness metastasis. Understanding these complex dynamics offers new therapeutic targets for disrupting TAM-mediated pathways overcoming drug resistance. underscores potential targeting to develop innovative therapies, emphasizing need further research into characteristics functional roles TME.
Язык: Английский
Процитировано
28
Biomarker Research, Год журнала: 2024, Номер 12(1)
Опубликована: Сен. 27, 2024
Abstract Cells, as the fundamental units of life, contain multidimensional spatiotemporal information. Single-cell RNA sequencing (scRNA-seq) is revolutionizing biomedical science by analyzing cellular state and intercellular heterogeneity. Undoubtedly, single-cell transcriptomics has emerged one most vibrant research fields today. With optimization innovation technologies, intricate details concealed within cells are gradually unveiled. The combination scRNA-seq other multi-omics at forefront field. This involves simultaneously measuring various omics data individual cells, expanding our understanding across a broader spectrum dimensions. precisely captures aspects transcriptomes, immune repertoire, spatial information, temporal epitopes, in diverse contexts. In addition to depicting cell atlas normal or diseased tissues, it also provides cornerstone for studying differentiation development patterns, disease heterogeneity, drug resistance mechanisms, treatment strategies. Herein, we review traditional technologies outline latest advancements multi-omics. We summarize current status challenges applying biological clinical applications. Finally, discuss limitations potential strategies address them.
Язык: Английский
Процитировано
26
Cell, Год журнала: 2024, Номер 187(23), С. 6760 - 6779.e24
Опубликована: Сен. 30, 2024
The capability to spatially explore RNA biology in formalin-fixed paraffin-embedded (FFPE) tissues holds transformative potential for histopathology research. Here, we present pathology-compatible deterministic barcoding tissue (Patho-DBiT) by combining situ polyadenylation and computational innovation spatial whole transcriptome sequencing, tailored probe the diverse species clinically archived FFPE samples. It permits co-profiling of gene expression processing, unveiling region-specific splicing isoforms, high-sensitivity transcriptomic mapping clinical tumor stored 5 years. Furthermore, genome-wide single-nucleotide variants can be captured distinguish malignant subclones from non-malignant cells human lymphomas. Patho-DBiT also maps microRNA regulatory networks dynamics, decoding their roles tumorigenesis. Single-cell level dissects spatiotemporal cellular dynamics driving clonal architecture progression. stands poised as a valuable platform unravel rich aid pathology evaluation.
Язык: Английский
Процитировано
26
Frontiers in Immunology, Год журнала: 2024, Номер 15
Опубликована: Апрель 16, 2024
Tertiary lymphoid structure (TLS) is an ectopic lymphocyte aggregate formed in peripheral non-lymphoid tissues, including inflamed or cancerous tissue. Tumor-associated TLS serves as a prominent center of antigen presentation and adaptive immune activation within the periphery, which has exhibited positive prognostic value various cancers. In recent years, concept maturity regarding been proposed mature TLS, characterized by well-developed germinal centers, exhibits more potent tumor-suppressive capacity with stronger significance. Meanwhile, evidence showed that can be induced therapeutic interventions during cancer treatments. Thus, evaluation induce its formation are critical issues current research. this review, we aim to provide comprehensive summary existing classifications for strategies capable inducing tumors.
Язык: Английский
Процитировано
24
Nature Reviews Clinical Oncology, Год журнала: 2024, Номер 21(9), С. 660 - 674
Опубликована: Июль 23, 2024
Язык: Английский
Процитировано
24
npj Imaging, Год журнала: 2024, Номер 2(1)
Опубликована: Июль 17, 2024
Abstract The recent upswing in the integration of spatial multi-omics for conducting multidimensional information measurements is opening a new chapter biological research. Mapping landscape various biomolecules including metabolites, proteins, nucleic acids, etc., and even deciphering their functional interactions pathways believed to provide more holistic nuanced exploration molecular intricacies within living systems. Mass spectrometry imaging (MSI) stands as forefront technique spatially mapping metabolome, lipidome, proteome diverse tissue cell samples. In this review, we offer systematic survey delineating different MSI techniques resolved analysis, elucidating principles, capabilities, limitations. Particularly, focus on advancements methodologies aimed at augmenting sensitivity specificity MSI; depict burgeoning MSI-based metabolomics, lipidomics, proteomics, encompassing synergy with other modalities. Furthermore, speculative insights into potential trajectory technology future.
Язык: Английский
Процитировано
23