Trends in Pharmacological Sciences,
Год журнала:
2024,
Номер
45(4), С. 304 - 318
Опубликована: Март 6, 2024
Breast
cancer's
tendency
to
metastasize
poses
a
critical
barrier
effective
treatment,
making
it
leading
cause
of
mortality
among
women
worldwide.
A
growing
body
evidence
is
showing
that
translational
adaptation
emerging
as
key
mechanism
enabling
cancer
cells
thrive
in
the
dynamic
tumor
microenvironment
(TME).
Here,
we
systematically
summarize
how
breast
utilize
drive
metastasis,
highlighting
intricate
regulation
by
specific
translation
machinery
and
mRNA
attributes
such
sequences
structures,
along
with
involvement
tRNAs
other
trans-acting
RNAs.
We
provide
an
overview
latest
findings
concepts
this
area,
discussing
their
potential
implications
for
therapeutic
strategies
cancer.
Cell,
Год журнала:
2024,
Номер
187(6), С. 1490 - 1507.e21
Опубликована: Март 1, 2024
Cell
cycle
progression
relies
on
coordinated
changes
in
the
composition
and
subcellular
localization
of
proteome.
By
applying
two
distinct
convolutional
neural
networks
images
millions
live
yeast
cells,
we
resolved
proteome-level
dynamics
both
concentration
during
cell
cycle,
with
resolution
∼20
classes.
We
show
that
a
quarter
proteome
displays
periodicity,
proteins
tending
to
be
controlled
either
at
level
or
concentration,
but
not
both.
Distinct
levels
protein
regulation
are
preferentially
utilized
for
different
aspects
being
mostly
involved
control
biophysical
implementation
program.
present
resource
exploring
global
which
will
aid
understanding
fundamental
biological
process
systems
level.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 7, 2024
Abstract
Characterizing
the
transcriptional
and
translational
gene
expression
patterns
at
single-cell
level
within
their
three-dimensional
(3D)
tissue
context
is
essential
for
revealing
how
genes
shape
structure
function
in
health
disease.
However,
most
existing
spatial
profiling
techniques
are
limited
to
5-20
µm
thin
sections.
Here,
we
developed
Deep-STARmap
Deep-RIBOmap,
which
enable
3D
situ
quantification
of
thousands
transcripts
corresponding
translation
activities,
respectively,
200-µm
thick
blocks.
This
achieved
through
scalable
probe
synthesis,
hydrogel
embedding
with
efficient
anchoring,
robust
cDNA
crosslinking.
We
first
utilized
combination
multicolor
fluorescent
protein
imaging
simultaneous
molecular
cell
typing
neuron
morphology
tracing
mouse
brain.
also
demonstrate
that
facilitates
comprehensive
quantitative
analysis
tumor-immune
interactions
human
skin
cancer.
Advanced Functional Materials,
Год журнала:
2024,
Номер
34(26)
Опубликована: Фев. 22, 2024
Abstract
Synthetic
biochemical
circuits
(e.g.,
DNA
circuits)
remain
at
the
forefront
of
intracellular
biosensing
tasks
yet
are
hindered
by
undesired
off‐site
activation
and
accompanying
signal
leakage.
Herein,
study
attempts
to
overcome
this
limitation
developing
a
simple‐yet‐powerful
endogenous
glutathione
(GSH)‐regulating
tactic
that
permits
robust
distinguishable
on‐site
microRNA
(miRNA)
imaging
under
disturbed
redox
homeostasis.
Specifically,
hierarchically
activated
catalytic
(HAD)
circuit
is
fabricated
grafting
disulfide
linkage
within
entropy‐driven
circuitry
(EDC)
reactants.
It
exemplified
HAD
system
promises
spatiotemporally
selective
microRNA‐21
(miR‐21)
in
living
cells
differentiation
tumor
from
normal
cells.
The
correlationship
between
GSH
miRNA
extensively
explored
live
cells,
can
substantially
expand
toolbox
for
profiling
processes.
Journal of genetics and genomics/Journal of Genetics and Genomics,
Год журнала:
2023,
Номер
51(4), С. 367 - 378
Опубликована: Авг. 1, 2023
Messenger
RNA
(mRNA)
translation
consists
of
initiation,
elongation,
termination,
and
ribosome
recycling,
carried
out
by
the
machinery,
primarily
including
tRNAs,
ribosomes,
factors
(TrFs).
Translational
regulators
transduce
signals
growth
development,
as
well
biotic
abiotic
stresses,
to
where
global
or
selective
translational
control
occurs
modulate
mRNA
efficiency
(TrE).
As
basis
control,
machinery
directly
determines
quality
quantity
newly
synthesized
peptides
and,
ultimately,
cellular
adaption.
Thus,
regulating
availability
diverse
components
is
reviewed
central
strategy
control.
We
provide
classical
signaling
pathways
(e.g.,
integrated
stress
responses)
behaviors
liquid-liquid
phase
separation)
exemplify
this
within
different
physiological
contexts,
particularly
during
host-microbe
interactions.
With
new
technologies
developed,
further
understanding
will
speed
up
medicine
agriculture.
Single
cell
RNA
sequencing
(scRNA-seq)
provides
a
great
convenience
for
studying
tumor
occurrence
and
development
its
ability
to
study
gene
expression
at
the
individual
level.
However,
patient-derived
tissues
are
composed
of
multiple
types
cells
including
adjacent
non-malignant
such
as
stromal
immune
cells.
The
spatial
locations
various
in
situ
plays
pivotal
role
tumors,
which
cannot
be
elucidated
by
scRNA-seq
alone.
Spatially
resolved
transcriptomics
(SRT)
technology
emerges
timely
explore
unrecognized
relationship
between
background
particular
functions,
is
increasingly
used
cancer
research.
This
review
systematic
overview
SRT
technologies
that
developed,
more
widely
cutting-edge
based
on
next-generation
(NGS).
In
addition,
main
achievements
precisely
unveiling
underappreciated
function
with
unprecedented
high-resolution
research
emphasized,
aim
developing
effective
clinical
therapeutics
oriented
deeper
understanding
interaction
surrounding
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Фев. 8, 2024
Spatial
transcriptomics
has
emerged
as
a
powerful
tool
for
dissecting
spatial
cellular
heterogeneity
but
of
today
is
largely
limited
to
gene
expression
analysis.
Yet,
the
life
RNA
molecules
multifaceted
and
dynamic,
requiring
profiling
different
species
throughout
cycle
delve
into
intricate
biology
in
complex
tissues.
Human
disease-relevant
tissues
are
commonly
preserved
formalin-fixed
paraffin-embedded
(FFPE)
blocks,
representing
an
important
resource
human
tissue
specimens.
The
capability
spatially
explore
FFPE
holds
transformative
potential
research
clinical
histopathology.
Here,
we
present
Patho-DBiT
combining
situ
polyadenylation
deterministic
barcoding
full
coverage
transcriptome
sequencing,
tailored
probing
diverse
landscape
even
clinically
archived
samples.
It
permits
co-profiling
processing,
unveiling
region-specific
splicing
isoforms,
high-sensitivity
transcriptomic
mapping
tumor
stored
five
years.
Furthermore,
genome-wide
single
nucleotide
variants
can
be
captured
distinguish
malignant
clones
from
non-malignant
cells
lymphomas.
also
maps
microRNA-mRNA
regulatory
networks
dynamics,
decoding
their
roles
tumorigenesis
trajectory.
High
resolution
at
level
reveals
neighborhood
traces
spatiotemporal
kinetics
driving
progression.
stands
poised
valuable
platform
unravel
rich
study
aid
pathology
evaluation.
Biology,
Год журнала:
2024,
Номер
13(6), С. 451 - 451
Опубликована: Июнь 19, 2024
Single-cell
RNA
sequencing
technology
(scRNA-seq)
has
been
steadily
developing
since
its
inception
in
2009.
Unlike
bulk
RNA-seq,
scRNA-seq
identifies
the
heterogeneity
of
tissue
cells
and
reveals
gene
expression
changes
individual
at
microscopic
level.
Here,
we
review
development
scRNA-seq,
which
gone
through
iterations
reverse
transcription,
vitro
smart-seq,
drop-seq,
10
×
Genomics,
spatial
single-cell
transcriptome
technologies.
The
Genomics
widely
applied
medicine
biology,
producing
rich
research
results.
Furthermore,
this
presents
a
summary
analytical
process
for
data
integration
with
other
omics
analyses,
including
genomes,
epigenomes,
proteomes,
metabolomics.
wide
range
applications
biology
medicine.
This
analyzes
cancer,
stem
cell
research,
developmental
microbiology,
fields.
In
essence,
provides
means
elucidating
patterns
single
cells,
thereby
offering
valuable
tool
scientific
research.
Nevertheless,
current
is
still
imperfect,
shortcomings
anticipates
future
developments.
objective
to
facilitate
deeper
comprehension
biological
medical
as
well
identify
avenues
alignment
practical
needs.
