Genomic convergence in terrestrial root plants through tandem duplication in response to soil microbial pressures

Cell Reports, Год журнала: 2024, Номер 43(10), С. 114786 - 114786

Опубликована: Сен. 26, 2024

Язык: Английский

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Speciation Genes

Elsevier eBooks, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Язык: Английский

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Genetic basis of an adaptive polymorphism controlling butterfly silver iridescence

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Дек. 17, 2024

Summary Identifying the genes and mutations that drive phenotypic variation which are subject to selection is crucial for understanding evolutionary processes. Mormon Fritillary butterflies ( Speyeria mormonia ) exhibit a striking wing color polymorphism throughout their range: typical morphs bear silver spots on ventral surfaces, can co-occur with unsilvered displaying dull coloration 1 . Through genome-wide association studies in two polymorphic populations, we fine-map this difference silvering 3’ region of transcription factor gene optix The expression confined regions surround spots, these patterns transformed identity upon RNAi knockdown, implicating as repressor scales butterfly. We show haplotype shows signatures recent selective sweeps, allele shared monomorphic, species hydaspe , suggesting introgressions facilitate exchange variants adaptive potential across species. Remarkably, findings parallel role cis -regulatory modulation shaping aposematic red Heliconius 2–7 lineage separated from 45 million years ago 8 genetic basis thus be more predictable than often presumed, even traits appear divergent large distances. Graphical Abstract

Язык: Английский

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Cis-regulatory modes of Ultrabithorax inactivation in butterfly forewings

Опубликована: Янв. 10, 2024

Hox gene clusters encode transcription factors that drive regional specialization during animal development: e.g. the factor Ubx is expressed in insect metathoracic (T3) wing appendages and differentiates them from T2 mesothoracic identities. transcriptional regulation requires silencing activities prevent spurious activation regulatory crosstalks wrong tissues, but this has seldom been studied insects other than Drosophila , which shows a derived dislocation into two genomic disjoined Antennapedia ( Antp ) Ultrabithorax ). Here we investigated how restricted to hindwing butterflies, amidst contiguous cluster. By analysing Hi-C ATAC-seq data butterfly Junonia coenia show Topologically Associated Domain (TAD) maintains hindwing-enriched profile of chromatin opening around . This TAD bordered by Boundary Element (BE) separates it region joined activity locus. CRISPR mutational perturbation BE releases ectopic expression forewings, inducing homeotic clones with Further interrogation non-coding RNA encoding regions one putative cis- module within cause rare transformations both directions, indicating presence activating repressing features. We also describe series spontaneous forewing phenotypes obtained Heliconius discuss their possible basis. leveraging extensive found our initial exploration demonstrates existence insulating sequences its forewings.

Язык: Английский

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eLife assessment: Cis-regulatory modes of Ultrabithorax inactivation in butterfly forewings

Опубликована: Янв. 10, 2024

Hox gene clusters encode transcription factors that drive regional specialization during animal development: e.g. the factor Ubx is expressed in insect metathoracic (T3) wing appendages and differentiates them from T2 mesothoracic identities. transcriptional regulation requires silencing activities prevent spurious activation regulatory crosstalks wrong tissues, but this has seldom been studied insects other than Drosophila, which shows a derived dislocation into two genomic disjoined Antennapedia (Antp) Ultrabithorax (Ubx). Here we investigated how restricted to hindwing butterflies, amidst contiguous cluster. By analysing Hi-C ATAC-seq data butterfly Junonia coenia, show Topologically Associated Domain (TAD) maintains hindwing-enriched profile of chromatin opening around Ubx. This TAD bordered by Boundary Element (BE) separates it region joined activity Antp locus. CRISPR mutational perturbation BE releases ectopic expression forewings, inducing homeotic clones with Further interrogation non-coding RNA encoding regions one putative cis-regulatory module within cause rare transformations both directions, indicating presence activating repressing features. We also describe series spontaneous forewing phenotypes obtained Heliconius discuss their possible basis. leveraging extensive found our initial exploration demonstrates existence insulating sequences its forewings.

Язык: Английский

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