Modeling Arabidopsis root growth and development DOI Creative Commons
Marta Ibañes

PLANT PHYSIOLOGY, Год журнала: 2025, Номер 197(2)

Опубликована: Фев. 1, 2025

Modeling has been used to explore various aspects of primary root development and growth in Arabidopsis thaliana, thanks enormous advances the genetic biochemical bases cell division, differentiation, and, more recently, progress measuring these processes. facilitated characterization regulations involved processes system properties that they confer. Recently, mechanical-physical have started be determined with help modeling. Here we review recent modeling approaches examine growth, from transcriptional signaling regulation decisions mechanical basis morphogenesis, highlight common features future challenges.

Язык: Английский

Developmental timing in plants DOI Creative Commons
Enrico Coen, Przemysław Prusinkiewicz

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Март 27, 2024

Plants exhibit reproducible timing of developmental events at multiple scales, from switches in cell identity to maturation the whole plant. Control likely evolved for similar reasons that humans invented clocks: coordinate events. However, whereas clocks are designed run independently conditions, plant is strongly dependent on growth and environment. Using simplified models convey key concepts, we review how growth-dependent inherent mechanisms interact with environment control cyclical progressive transitions plants.

Язык: Английский

Процитировано

8
Top five unanswered questions in plant cell surface research DOI Creative Commons
Wout Boerjan, Vincent Burlat, Daniel J. Cosgrove

и другие.

The Cell Surface, Год журнала: 2024, Номер 11, С. 100121 - 100121

Опубликована: Фев. 13, 2024

Plant cell wall researchers were asked their view on what the major unanswered questions are in field. This article summarises feedback that was received from them five questions. In this issue you can find equivalent syntheses for working bacterial, unicellular parasite and fungal systems.

Язык: Английский

Процитировано

7
Growth and tension in explosive fruit DOI Creative Commons
Gabriella Mosca, Ryan C. Eng,

Milad Adibi

и другие.

Current Biology, Год журнала: 2024, Номер 34(5), С. 1010 - 1022.e4

Опубликована: Фев. 14, 2024

Exploding seed pods of the common weed Cardamine hirsuta have remarkable ability to launch seeds far from plant. The energy for this explosion comes tension that builds up in fruit valves. Above a critical threshold, fractures along its dehiscence zone and two valves coil explosively, ejecting seeds. A mechanism generate is drying, causing tissues shrink. However, does not happen C. fruit. Instead, produced by active contraction growing exocarp cells outer layer Exactly how growth causes tissue contract pulling force unknown. Here we show reorientation microtubules cell cortex changes orientation cellulose microfibrils wall consequent cellular pattern. We used mechanical modeling emerges through due highly anisotropic load-bearing their effect on shape. By explicitly defining as multi-layered our model, discovered cross-lamellate pattern further enhances developing cells. Therefore, interplay properties with turgor-driven enables sufficient power explosive dispersal.

Язык: Английский

Процитировано

7
Molecular-level carbon traits underlie the multidimensional fine root economics space DOI
Mengke Wang, Deliang Kong, Xiaohan Mo

и другие.

Nature Plants, Год журнала: 2024, Номер 10(6), С. 901 - 909

Опубликована: Май 13, 2024

Язык: Английский

Процитировано

6
Deformable microlaser force sensing DOI Creative Commons
Eleni Dalaka,

Joseph Hill,

Jonathan H. Booth

и другие.

Light Science & Applications, Год журнала: 2024, Номер 13(1)

Опубликована: Июнь 5, 2024

Abstract Mechanical forces are key regulators of cellular behavior and function, affecting many fundamental biological processes such as cell migration, embryogenesis, immunological responses, pathological states. Specialized force sensors imaging techniques have been developed to quantify these otherwise invisible in single cells vivo. However, current rely heavily on high-resolution microscopy do not allow interrogation optically dense tissue, reducing their application 2D cultures highly transparent tissue. Here, we introduce DEFORM , deformable microlaser sensing a spectroscopic technique that detects sub-nanonewton with unprecedented spatio-temporal resolution. is based the spectral analysis laser emission from dye-doped oil microdroplets uses force-induced lifting mode degeneracy droplets detect nanometer deformations. Following validation by atomic development model links changes spectrum applied force, used measure 3D at depths hundreds microns within tumor spheroids late-stage Drosophila larva. We furthermore show continuous single-cell spatial millisecond temporal resolution, thus paving way for non-invasive studies biomechanical advanced stages tissue remodeling, invasion.

Язык: Английский

Процитировано

6
Grasses exploit geometry to achieve improved guard cell dynamics DOI Creative Commons
Clinton H. Durney, Matthew J. Wilson,

Shauni McGregor

и другие.

Current Biology, Год журнала: 2023, Номер 33(13), С. 2814 - 2822.e4

Опубликована: Июнь 15, 2023

Stomata are controllable micropores formed between two adjacent guard cells (GCs) that regulate gas flow across the plant surface.1Hetherington A.M. Woodward F.I. The role of stomata in sensing and driving environmental change.Nature. 2003; 424: 901-908Crossref PubMed Scopus (1589) Google Scholar Grasses, among most successful organisms on planet main food crops for humanity, have GCs flanked by specialized lateral subsidiary (SCs).2Nunes T.D.G. Zhang D. Raissig M.T. Form, development function grass stomata.Plant J. 2020; 101: 780-799Crossref (42) Scholar,3Raissig Abrash E. Bettadapur A. Vogel J.P. Bergmann D.C. Grasses use an alternatively wired bHLH transcription factor network to establish stomatal identity.Proc. Natl. Acad. Sci. USA. 2016; 113: 8326-8331Crossref (96) Scholar,4McKown K.H. Grass stomata.Curr. Biol. 2018; 28: R814-R816Abstract Full Text PDF (0) SCs improve performance acting as a local pool ions metabolites drive changes turgor pressure within open/close pore.4McKown Scholar,5McAusland L. Vialet-Chabrand S. Davey P. Baker N.R. Brendel O. Lawson T. Effects kinetics light-induced responses photosynthesis water-use efficiency.New Phytol. 211: 1209-1220Crossref (223) Scholar,6Raschke K. Fellows M.P. Stomatal movement Zea mays: shuttle potassium chloride cells.Planta. 1971; 296-316Crossref (124) Scholar,7Schäfer N. Maierhofer Herrmann Jørgensen M.E. Lind C. von Meyer Lautner Fromm Felder M. Hetherington et al.A tandem amino acid residue motif cell SLAC1 anion channel grasses allows control aperture nitrate.Curr. 1370-1379.e5Abstract (33) Scholar,8Mumm Wolf Roelfsema M.R. Marten I. Cell type-specific regulation ion channels maize complex.Plant Physiol. 2011; 52: 1365-1375Crossref 4-celled complex also involves distinctive geometry, having dumbbell-shaped compared with typical kidney-shaped stomata.2Nunes Scholar,9Franks P.J. Farquhar G.D. mechanical diversity its significance gas-exchange control.Plant 2007; 143: 78-87Crossref (406) However, degree which this geometry contributes improved performance, underlying mechanism, remains unclear. To address question, we created finite element method (FEM) model successfully captures experimentally observed pore opening/closure. Exploration model, including silico experimental mutant analyses, supports importance reciprocal system effective function, functioning springs restrain GC movement. Our results show not essential but lead more responsive system. In addition, wall anisotropy is required (in contrast GCs10Woolfenden H.C. Bourdais G. Kopischke Miedes Molina Robatzek Morris R.J. A computational approach inferring properties govern dynamics.Plant 2017; 92: 5-18Crossref (32) Scholar) relatively thick rod region needed enhance opening. demonstrate specific cellular associated stomata.

Язык: Английский

Процитировано

14
Dynamic Structural Change of Plant Epidermal Cell Walls under Strain DOI Creative Commons
Jingyi Yu, Joshua T. Del Mundo, Guillaume Freychet

и другие.

Small, Год журнала: 2024, Номер 20(30)

Опубликована: Фев. 22, 2024

Abstract The molecular foundations of epidermal cell wall mechanics are critical for understanding structure–function relationships primary walls in plants and facilitating the design bioinspired materials. To uncover mechanisms regulating high extensibility strength wall, onion is stretched uniaxially to various strains structures from mesoscale atomic scale characterized. Upon longitudinal stretching strain, contract transverse direction, resulting a reduced area. Atomic force microscopy shows that cellulose microfibrils exhibit orientation‐dependent rearrangements at strains: straightened out become highly ordered, while curve kink. Small‐angle X‐ray scattering detects 7.4 nm spacing aligned along stretch direction which attributed distances between individual microfibrils. Furthermore, wide‐angle reveals widening (004) lattice contraction (200) longitudinally implies crystal. These findings provide insights into ability bear additional load after yielding: aggregation impedes sliding enables further increased loads.

Язык: Английский

Процитировано

5
Creating an explosion: Form and function in explosive fruit DOI Creative Commons

Erin Cullen,

Angela Hay

Current Opinion in Plant Biology, Год журнала: 2024, Номер 79, С. 102543 - 102543

Опубликована: Апрель 30, 2024

Adaptations for seed dispersal are found everywhere in nature. However, only a fraction of this diversity is accessible through the study model organisms. For example, Arabidopsis seeds released by dehiscent fruit; and although many genes required dehiscence have been identified, genetic basis vast majority strategies remains understudied. Explosive fruit generate mechanical forces to launch over wide area. Recent work indicates that key innovations explosive lie localised lignin deposition precise patterns microtubule-dependent growth valves, rather than zone structure. These insights come from comparative approaches, which extend reach developmental genetics developing experimental tools less well-studied species, such as relative, Cardamine hirsuta.

Язык: Английский

Процитировано

5
Cell wall dynamics: novel tools and research questions DOI Creative Commons
Luis Alonso Baez, Laura Bacete

Journal of Experimental Botany, Год журнала: 2023, Номер 74(21), С. 6448 - 6467

Опубликована: Авг. 2, 2023

Abstract Years ago, a classic textbook would define plant cell walls based on passive features. For instance, sort of exoskeleton invariable polysaccharide composition, and probably painted in green. However, currently, this view has been expanded to consider as active, heterogeneous, dynamic structures with high degree complexity. what do we mean when refer wall structure? How can investigate the different implications dynamism? While first question subject several recent publications, defining ideal strategies tools needed address second proven be challenging due myriad techniques available. In review, will describe capacities methodologies study structure, other aspects developed or optimized years. Keeping mind dynamism plasticity, advantages performing long-term non-invasive live-imaging methods emphasized. We specifically focus for Arabidopsis thaliana primary walls, but could applied both secondary species. believe toolset help researchers expanding knowledge these dynamic/evolving structures.

Язык: Английский

Процитировано

12
A CUC1/auxin genetic module links cell polarity to patterned tissue growth and leaf shape diversity in crucifer plants DOI Creative Commons
Ziliang Hu, David Wilson‐Sánchez, Neha Bhatia

и другие.

Proceedings of the National Academy of Sciences, Год журнала: 2024, Номер 121(26)

Опубликована: Июнь 21, 2024

How tissue-level information encoded by fields of regulatory gene activity is translated into the patterns cell polarity and growth that generate diverse shapes different species remains poorly understood. Here, we investigate this problem in case leaf shape differences between

Язык: Английский

Процитировано

4